[12] Isolation and characterization of colicin fragments of bacterial 16S ribosomal RNA

Typical symptoms of phytoplasma infection were observed on 11 important crops in Oman that included alfalfa, sesame, chickpea, eggplant, tomato, spinach, rocket, carrot, squash, field pea, and faba bean. To identify the phytoplasmas in these crops, samples from infected and asymptomatic plants were collected, followed by amplifying and sequencing of the 16S ribosomal RNA, secA, tuf, imp, and SAP11 genes. We found that these sequences share >99% similarity with the peanut witches’ broom subgroup (16SrII-D). Whereas some sequence variation was found in the five genes among 11 phytoplasma isolates of different crops, all sequences grouped into one clade along with those of other phytoplasmas belonging to the 16SrII-D group. Thus, 16SrII-D phytoplasmas infect a diverse range of crops in Oman. Phytoplasmas in this group have not been reported to occur in carrot, spinach, rocket, and field pea previously. Within Oman, this is the first report of the presence of 16SrII-D phytoplasmas in tomato, spinach, rocket, carrot, squash, field pea, and faba bean. Sequences of the five genes enabled for better distinction of the 16SrII-D phytoplasmas that occur in Oman.

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Characterization of the 16S ribosomal RNA genes and phylogenetic relationships of sulfur-dependent thermoacidophilic archaebacteria.

The Journal of General and Applied Microbiology ◽

10.2323/jgam.41.75 ◽

1995 ◽

Vol 41 (1) ◽

pp. 75-81 ◽

Cited By ~ 7

Author(s):

NORIO KUROSAWA ◽

KIYOTAKA OHKURA ◽

TADAO HORIUCHI ◽

YUKO H. ITOH

Keyword(s):

Ribosomal Rna ◽

Phylogenetic Relationships ◽

Ribosomal Rna Genes ◽

16S Ribosomal Rna ◽

Rna Genes

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Isolation and Characterization of Ribosomal RNA from the Phycomycete Allomyces arbuscula / Isolation and Characterization of Ribosomal RNA from the Phycomycete Allomyces arbuscula

Zeitschrift für Naturforschung C ◽

10.1515/znc-1975-3-430 ◽

1975 ◽

Vol 30 (3-4) ◽

pp. 302-303 ◽

Cited By ~ 1

Author(s):

P. Fähnrich ◽

H.H. Rothe ◽

M. Trapp

Keyword(s):

Ribosomal Rna ◽

Base Composition ◽

18S Rrna ◽

Isolation And Characterization ◽

Allomyces Arbuscula

Abstract The ribosomal RNA of the gametophyte of Allomyces arbuscula contains two components with sedimentation coefficients of approximately 25S and 18S. The percent base composition of 25S rRNA is 31/28/24/17 (U/G/A/C), that of 18S rRNA 34/28/22/16

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Synthesis and characterization of modified nucleotides in the 970 hairpin loop of Escherichia coli 16S ribosomal RNA

Bioorganic & Medicinal Chemistry ◽

10.1016/j.bmc.2009.07.008 ◽

2009 ◽

Vol 17 (16) ◽

pp. 5887-5893 ◽

Cited By ~ 7

Author(s):

N. Dinuka Abeydeera ◽

Christine S. Chow

Keyword(s):

Escherichia Coli ◽

Ribosomal Rna ◽

16S Ribosomal Rna ◽

Synthesis And Characterization ◽

Hairpin Loop ◽

Modified Nucleotides

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Cultural and molecular characterization of photobionts of Peltigera Membranacea

The Lichenologist ◽

10.1006/lich.1997.0114 ◽

1997 ◽

Vol 29 (6) ◽

pp. 571-586 ◽

Cited By ~ 24

Author(s):

V.P.W. Mioa ◽

A. Rabenau ◽

A. Lee

Keyword(s):

16S Rdna ◽

Ribosomal Rna ◽

Molecular Study ◽

16S Ribosomal Rna ◽

Chain Reaction ◽

Ribosomal Rna Gene ◽

Pcr Product ◽

16S Ribosomal Rna Gene ◽

Polymerase Chain

AbstractA molecular study was undertaken to clarify the identity of the photobiont in colourmorphs of the lichen, Peltigera membranacea. Two strains of cyanobacteria, identified as Nosroc sp. by morphology, were cultivated from each of two lichen specimens. Prokaryotic (16S) ribosomal RNA gene fragments were amplified by the polymerase chain reaction (PCR) from DNA extracted from the isolated strains and the lichens, and sequenced directly. Sequences were 98 1% identical between lichen specimens, TDI#AR94 and TDI#AR95, and highly similar to sequences published, or generated in this study from a type culture, for Nostoc. The 16S ribosomal RNA gene sequences (‘ 16S rDNA’) of all four lichen-derived cyanobacteria appeared the same, even though the lichen specimens from which they originated had different sequences. The 16S rDXA from strains 9A and 9B were different from that of specimen TDI#AR94, the thallus from which they were isolated, and instead were the same as that of strains 10A and 10B, and their source, specimen TDI#AR95. When primers selective for the strain 9A sequence were used, however, a small amount of PCR product corresponding to the 16S rDNA of strain 9A was obtained from lichen TDI#AR94. The results confirm that the photobionts of P. membranacea belong to Nostoc, and suggest that genetic differences in the photobiont may be a factor in the occurrence of colourmorphs among cyanolichens.

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FRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs

ACS Chemical Biology ◽

10.1021/acschembio.5b00952 ◽

2016 ◽

Vol 11 (5) ◽

pp. 1347-1353 ◽

Cited By ~ 27

Author(s):

Thomas Lavergne ◽

Rajan Lamichhane ◽

Denis A. Malyshev ◽

Zhengtao Li ◽

Lingjun Li ◽

...

Keyword(s):

Ribosomal Rna ◽

Conformational Changes ◽

16S Ribosomal Rna ◽

Base Pairs

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Isolation and characterization of Lactobacillus microflora from homemade white sheep’s milk cheeses during ripening

Agricultural Science and Technology ◽

10.15547/ast.2021.01.015 ◽

2021 ◽

pp. 79-82

Author(s):

S. Stojanovski ◽

G. Cilev ◽

B. Trajanoska

Keyword(s):

Lactobacillus Plantarum ◽

Ribosomal Rna ◽

Lactobacillus Curvatus ◽

Specific Primers ◽

Maturation Period ◽

Cheese Ripening ◽

Isolation And Characterization ◽

White Cheese ◽

Lactobacillus Spp

Abstract. The Lactobacillus microflora of white cheese made from sheep’s milk was studied during the maturation period of 10 to 100 days in the basement at a temperature of 10-12°C. Eight samples from different stages of natural white cheese ripening were taken for testing. A total of 28 strains were isolated and identified as Lactobacillus spp. based on their growth, gram-stain activity, catalase and oxidase. Their affiliation to this genus was confirmed by PCR with genus specific primers, 16S ribosomal RNA. The results show that in the early stages of cheese ripening (5th-6th, 10-39 day) the most commonly found species are: Lactobacillus plantarum and Lactobacillus curvatus, while in later stages of the cheese ripening (7th-10th, 40-80 day), the following species are found: Lactobacillus plantarum and Lactobacillus paracasei subsp. Paracasei.

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