scholarly journals Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA

1989 ◽  
Vol 17 (19) ◽  
pp. 7843-7853 ◽  
Author(s):  
Ulrike Edwards ◽  
Till Rogall ◽  
Helmut Blöcker ◽  
Monica Emde ◽  
Erik C. Böttger
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Gene Coding

scholarly journals Detection, Identification, and Molecular Characterization of the 16SrII-D Phytoplasmas Infecting Vegetable and Field Crops in Oman

Plant Disease ◽  
2018 ◽  
Vol 102 (3) ◽  
pp. 576-588 ◽  
Author(s):  
Ali M. Al-Subhi ◽  
Saskia A. Hogenhout ◽  
Rashid A. Al-Yahyai ◽  
Abdullah M. Al-Sadi
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Rna ◽  
Faba Bean ◽  
Sequence Variation ◽  
Field Pea ◽  
16S Ribosomal Rna ◽  
Diverse Range ◽  
Field Crops ◽  
Phytoplasma Infection

Typical symptoms of phytoplasma infection were observed on 11 important crops in Oman that included alfalfa, sesame, chickpea, eggplant, tomato, spinach, rocket, carrot, squash, field pea, and faba bean. To identify the phytoplasmas in these crops, samples from infected and asymptomatic plants were collected, followed by amplifying and sequencing of the 16S ribosomal RNA, secA, tuf, imp, and SAP11 genes. We found that these sequences share >99% similarity with the peanut witches’ broom subgroup (16SrII-D). Whereas some sequence variation was found in the five genes among 11 phytoplasma isolates of different crops, all sequences grouped into one clade along with those of other phytoplasmas belonging to the 16SrII-D group. Thus, 16SrII-D phytoplasmas infect a diverse range of crops in Oman. Phytoplasmas in this group have not been reported to occur in carrot, spinach, rocket, and field pea previously. Within Oman, this is the first report of the presence of 16SrII-D phytoplasmas in tomato, spinach, rocket, carrot, squash, field pea, and faba bean. Sequences of the five genes enabled for better distinction of the 16SrII-D phytoplasmas that occur in Oman.

scholarly journals 16S ribosomal RNA sequence analysis for determination of phylogenetic relationship among methylotrophs

1990 ◽  
Vol 136 (1) ◽  
pp. 1-10 ◽  
Author(s):  
K. Tsuji ◽  
H. C. Tsien ◽  
R. S. Hanson ◽  
S. R. DePalma ◽  
R. Scholtz ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Relationship ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Rna Sequence

Cultural and molecular characterization of photobionts of Peltigera Membranacea

1997 ◽  
Vol 29 (6) ◽  
pp. 571-586 ◽  
Author(s):  
V.P.W. Mioa ◽  
A. Rabenau ◽  
A. Lee
Keyword(s):  
16S Rdna ◽  
Ribosomal Rna ◽  
Molecular Study ◽  
16S Ribosomal Rna ◽  
Chain Reaction ◽  
Ribosomal Rna Gene ◽  
Pcr Product ◽  
16S Ribosomal Rna Gene ◽  
Polymerase Chain

AbstractA molecular study was undertaken to clarify the identity of the photobiont in colourmorphs of the lichen, Peltigera membranacea. Two strains of cyanobacteria, identified as Nosroc sp. by morphology, were cultivated from each of two lichen specimens. Prokaryotic (16S) ribosomal RNA gene fragments were amplified by the polymerase chain reaction (PCR) from DNA extracted from the isolated strains and the lichens, and sequenced directly. Sequences were 98 1% identical between lichen specimens, TDI#AR94 and TDI#AR95, and highly similar to sequences published, or generated in this study from a type culture, for Nostoc. The 16S ribosomal RNA gene sequences (‘ 16S rDNA’) of all four lichen-derived cyanobacteria appeared the same, even though the lichen specimens from which they originated had different sequences. The 16S rDXA from strains 9A and 9B were different from that of specimen TDI#AR94, the thallus from which they were isolated, and instead were the same as that of strains 10A and 10B, and their source, specimen TDI#AR95. When primers selective for the strain 9A sequence were used, however, a small amount of PCR product corresponding to the 16S rDNA of strain 9A was obtained from lichen TDI#AR94. The results confirm that the photobionts of P. membranacea belong to Nostoc, and suggest that genetic differences in the photobiont may be a factor in the occurrence of colourmorphs among cyanolichens.

scholarly journals FRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs

2016 ◽  
Vol 11 (5) ◽  
pp. 1347-1353 ◽  
Author(s):  
Thomas Lavergne ◽  
Rajan Lamichhane ◽  
Denis A. Malyshev ◽  
Zhengtao Li ◽  
Lingjun Li ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Conformational Changes ◽  
16S Ribosomal Rna ◽  
Base Pairs
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