[1] Analysis of ascorbic acid, dehydroascorbic acid, and transformation products by ion-pairing high-performance liquid chromatography with multiwavelength ultraviolet and electrochemical detection

1997 ◽  
pp. 3-12 ◽  
Author(s):  
Eiji Kimoto ◽  
Shigeyuki Terada ◽  
Takeo Yamaguchi
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Dehydroascorbic Acid ◽  
Ion Pairing ◽  
Transformation Products

Ascorbate in plasma as measured by liquid chromatography and by dichlorophenolindophenol colorimetry.

1986 ◽  
Vol 32 (6) ◽  
pp. 1004-1006 ◽  
Author(s):  
D J VanderJagt ◽  
P J Garry ◽  
W C Hunt
Keyword(s):  
Ascorbic Acid ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Colorimetric Method ◽  
Dehydroascorbic Acid ◽  
High Performance Liquid Chromatographic ◽  
Plasma Samples ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract Ascorbic acid was measured in 125 plasma samples by an automated colorimetric method involving dichlorophenolindophenol and by a "high-performance" liquid-chromatographic procedure with electrochemical detection. The two methods gave comparable results for samples with ascorbate concentrations of 1 to 20 mg/L (r = 0.97). We also measured the amount of total ascorbate (ascorbic acid + dehydroascorbic acid) in the same samples by a liquid-chromatographic procedure with precolumn derivitization of ascorbic acid. We confirmed that plasma contains little dehydroascorbic acid.

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