1070 In utero induction of oral tolerarnce in rats: Antigen feeding during pregnancy downregulates specific IgE response in offspring

ABSTRACT Cholera toxin (CT)-specific antibody responses of the immunoglobulin E (IgE) isotype in the sera of adult patients suffering from infection with either Vibrio cholerae O1, V. cholerae O139, or enterotoxigenic Escherichia coli(ETEC) were analyzed and compared with those in the sera of volunteers immunized with a bivalent B subunit O1/O139 whole-cell cholera vaccine. A significant IgE response to CT was observed in 90% of the patients with V. cholerae O1 infection (18 of 20; P = <0.001) and 95% of the patients with V. cholerae O139 infection (19 of 20; P = <0.001). Similarly, the majority of the patients with ETEC diarrhea (83%; 13 of 15) showed a positive IgE response to CT. Eight of 10 North American volunteers (80%) orally challenged with V. cholerae O1 showed CT-specific IgE responses (P = 0.004). In contrast, Swedish volunteers immunized with the oral cholera vaccine showed no IgE responses to CT (P value not significant). During the study period, total IgE levels in the sera of the diarrheal patients, the North American volunteers, and the Swedish cholera vaccinees alike remained unchanged. However, the total IgE levels in the sera of patients and healthy Bangladeshi controls were on average 89-fold higher than those in the sera of the healthy Swedish volunteers and 34-fold higher than those in the sera of the North American volunteers.

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Lack of evidence of IgE allergic sensitisation from working with lactic acid bacteria in the dairy foods industry

Journal of Dairy Research ◽

10.1017/s0022029918000419 ◽

2018 ◽

Vol 85 (3) ◽

pp. 355-357

Author(s):

Coralie Barrera ◽

Gabriel Reboux ◽

Audrey Laboissière ◽

Laurence Millon ◽

Anne Oppliger

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Preventive Measures ◽

Immunoglobulin E ◽

Milk Powder ◽

Medical Practitioner ◽

Specific Ige ◽

Adverse Health Effects ◽

High Concentrations ◽

Ige Response

This research communication aimed to evaluate the level of immunoglobulin E from lactic acid bacteria (LAB) that are used in dairy industries. Previous studies have demonstrated that workers report symptoms of irritation and are frequently IgG-sensitised to LAB. Workers (n = 44) from a probiotic production unity and the control lab were seen by a medical practitioner and responded to an occupational questionnaire. Specific IgE by the DELFIA® technique against 6 strains of LAB were measured on 44 exposed workers and 31 controls sera. Levels of specific IgE were low and no difference was observed between the two groups. This lack of IgE response could be explained by a healthy worker effect, an efficient implementation of personal protective equipment or by an absence of allergic mechanisms to account for the self-reported irritative symptoms. Despite the high concentrations of LAB, preventive measures are effective enough to guarantee no allergic effect and to prevent other adverse health effects. The implementation of preventive measures to avoid or reduce exposure to dust of LAB, and more generally to milk powder, is recommended in all dairy industry.

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Induction of antigen-specific IgE response in murine lymphocytes by IL-10

Immunology Letters ◽

10.1016/0165-2478(95)00084-i ◽

1995 ◽

Vol 47 (1-2) ◽

pp. 127-132 ◽

Cited By ~ 20

Author(s):

Hitoshi Ohmori ◽

Tsutomu Kanda ◽

Toshiyuki Takai ◽

Masaki Hikida

Keyword(s):

Specific Ige ◽

Ige Response

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Carbon Nanofibers Have IgE Adjuvant Capacity but Are Less Potent Than Nanotubes in Promoting Allergic Airway Responses

BioMed Research International ◽

10.1155/2013/476010 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 10

Author(s):

Unni Cecilie Nygaard ◽

Mari Samuelsen ◽

Calin Daniel Marioara ◽

Martinus Løvik

Keyword(s):

Lung Inflammation ◽

Carbon Nanofiber ◽

Inflammatory Cells ◽

Health Impact ◽

Specific Ige ◽

Positive Control ◽

Bronchoalveolar Fluid ◽

Ige Response ◽

Airway Responses ◽

Airway Model

There is a growing concern for the possible health impact of nanoparticles. The main objective of this study was to investigate the allergy-promoting capacity of four different carbon nanofiber (CNF) samples in an injection and an airway mouse model of allergy. Secondly, the potency of the CNF was compared to the previously reported allergy-promoting capacity of carbon nanotubes (CNT) in the airway model. Ultrafine carbon black particles (ufCBP) were used as a positive control. Particles were given together with the allergen ovalbumin (OVA) either by subcutaneous injection into the footpad or intranasally to BALB/cA mice. After allergen booster, OVA-specific IgE, IgG1, and IgG2a in serum were measured. In the airway model, inflammation was determined as influx of inflammatory cells (eosinophils, neutrophils, lymphocytes, and macrophages) and by mediators (MCP-1 and TNF-αpresent in bronchoalveolar fluid (BALF)). CNF and CNT both increased OVA-specific IgE levels in the two models, but in the airway model, the CNT gave a significantly stronger IgE response than the CNF. Furthermore, the CNT and not the CNF promoted eosinophil lung inflammation. Our data therefore suggest that nanotube-associated properties are particularly potent in promoting allergic responses.

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