Enterotoxin-Specific Immunoglobulin E Responses in Humans after Infection or Vaccination with Diarrhea-Causing Enteropathogens

ABSTRACT Cholera toxin (CT)-specific antibody responses of the immunoglobulin E (IgE) isotype in the sera of adult patients suffering from infection with either Vibrio cholerae O1, V. cholerae O139, or enterotoxigenic Escherichia coli(ETEC) were analyzed and compared with those in the sera of volunteers immunized with a bivalent B subunit O1/O139 whole-cell cholera vaccine. A significant IgE response to CT was observed in 90% of the patients with V. cholerae O1 infection (18 of 20; P = <0.001) and 95% of the patients with V. cholerae O139 infection (19 of 20; P = <0.001). Similarly, the majority of the patients with ETEC diarrhea (83%; 13 of 15) showed a positive IgE response to CT. Eight of 10 North American volunteers (80%) orally challenged with V. cholerae O1 showed CT-specific IgE responses (P = 0.004). In contrast, Swedish volunteers immunized with the oral cholera vaccine showed no IgE responses to CT (P value not significant). During the study period, total IgE levels in the sera of the diarrheal patients, the North American volunteers, and the Swedish cholera vaccinees alike remained unchanged. However, the total IgE levels in the sera of patients and healthy Bangladeshi controls were on average 89-fold higher than those in the sera of the healthy Swedish volunteers and 34-fold higher than those in the sera of the North American volunteers.

Download Full-text

Prevalence of specific immunoglobulin E and G against Aspergillus fumigatus in patients with asthma

Current Medical Mycology ◽

10.18502/cmm.4.4.380 ◽

2019 ◽

Author(s):

Newsha Hedayati ◽

Vida Mortezaee ◽

Seyed Alireza Mahdaviani ◽

Maryam Sadat Mirenayat ◽

Maryam Hassanzad ◽

...

Keyword(s):

Aspergillus Fumigatus ◽

Severe Asthma ◽

Immunoglobulin E ◽

Clinical Symptoms ◽

Specific Ige ◽

Total Ige ◽

Asthmatic Patients ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin ◽

The Mean

Background and Purpose: Aspergillus fumigatus as a ubiquitous fungus can be found in the respiratory tract of the asthmatic and healthy people. The inhalation of Aspergillus spores leads to an immune response in individuals with asthma and results in the aggravation of the clinical symptoms. The present study aimed to investigate the prevalence of specific immunoglobulin E and G (IgE and IgG) against A.fumigatus in asthmatic patients.Materials and Methods: This study was conducted on 200 consecutive patients with moderate to severe asthma referring to Masih Daneshvari hospital Tehran, Iran, from January 2016 to February 2018. Skin prick test (SPT) was performed in all subjects with Aspergillus allergens. Moreover, all patients underwent specific IgE testing for Aspergillus using Hycor method. Enzyme immune assay was applied to measure total IgE and Aspergillus-specific IgG.Results: According to the results, the mean age of the patients was 45.8 years (age range: 18-78 years). The mean levels of total IgE and Aspergillus specific IgE in asthmatic patients were obtained as 316.3 (range: 6-1300 IU/ml) and 1.5 (range: 0.1-61.3 IU/ml), respectively. Out of 200 patients, 27 (13.5%), 65 (32.5%), 22 (11.0%), and 86 (43.0%) cases had positive Aspergillus SPT, total IgE of > 417 IU/ml, Aspergillus-specific IgE, and IgG, respectively. The level of these variables in patients with severe asthma were 16 (16.5%), 36 (37.1%), 15 (15.5%), and 46 (47.4%), respectively.Conclusion: As the findings indicated, reactivity to Aspergillus is a remarkable phenomenon in asthmatic patients. It is also emphasised that the climatic condition may affect the positive rate of hypersensitivity to Aspergillus.

Download Full-text

Evaluation of a fully automated and completely quantitative allergen-specific IgE and total IgE detection assay

Analytical Methods ◽

10.1039/d1ay01172c ◽

2021 ◽

Author(s):

Huimin Huang ◽

Zhifeng Huang ◽

Peiyan Zheng ◽

Nili Wei ◽

Xueqing Liang ◽

...

Keyword(s):

Immunoglobulin E ◽

Detection System ◽

Specific Ige ◽

Quantitative Detection ◽

Total Ige ◽

Detection Assay ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

Based on the current urgent need for an in vitro quantitative detection system for allergens in most hospitals in China, we introduced a novel allergen-specific immunoglobulin E detection system that...

Download Full-text

Dysregulated specific IgE production to bystander foods in children with peanut allergy but not egg allergy

Journal of Food Allergy ◽

10.2500/jfa.2021.3.210005 ◽

2021 ◽

Vol 3 (1) ◽

pp. 24-31 ◽

Cited By ~ 1

Author(s):

Nurcicek Padem ◽

Kristin Erickson ◽

Meagan Yong ◽

Melanie Makhija ◽

Kathryn E. Hulse ◽

...

Keyword(s):

Food Allergy ◽

Peanut Allergy ◽

Immunoglobulin E ◽

Specific Ige ◽

Egg White ◽

Total Ige ◽

Control Subjects ◽

Specific Immunoglobulin E ◽

Natural Tolerance ◽

And Control

Background: Food specific immunoglobulin E (sIgE) levels are associated with the development of allergic responses and are used in the clinical evaluation of food allergy. Food sIgG4 levels have been associated with tolerance or clinical nonresponsiveness, particularly in interventional studies. Objective: We aimed to characterize food-specific antibody responses and compare responses with different foods in food allergy. Methods: Serum sIgA, sIgG4, and sIgE to whole peanut, egg white, and wheat, along with total IgE were measured in 57 children. Children with food allergy, children with natural tolerance, and controls were studied. The Mann-Whitney test or Kruskall Wallis test with the Dunn correction were used for statistical analysis. Results: As expected, total IgE levels were highest in the subjects with food allergy compared with the subjects who were nonallergic (p < 0.001) or the subjects who were naturally tolerant (p < 0.001). Peanut sIgE levels were higher in subjects with peanut allergy compared with the subjects who were naturally tolerant (p < 0.0001) and the control subjects (p < 0.03). Interestingly, peanut sIgG4 levels were also highest in children with peanut allergy compared with subjects who were naturally tolerant and control subjects (p = 0.28 and p < 0.001, respectively). Subjects with peanut allergy alone had comparable egg white sIgE levels to children with egg white allergy. In addition, the subjects with peanut allergy alone also had higher levels of egg white and wheat sIgE compared with the control subjects (p < 0.02 and p = 0.001, respectively). In contrast, the subjects with egg white allergy did not demonstrate elevated peanut or wheat sIgE levels. Conclusion: These novel findings suggested that IgE production is dysregulated in patients with peanut allergy, who are much less likely to outgrow their allergy, and suggest that the mechanisms that drive more persistent forms of food allergy may be distinct from more transient forms of food allergy.

Download Full-text

Serum and Intestinal Antitoxin Antibody Responses after Immunization with the Whole-Cell/Recombinant B Subunit (WC/rBS) Oral Cholera Vaccine in North American and Mexican Volunteers

Journal of Travel Medicine ◽

10.1111/j.1708-8305.1996.tb00728.x ◽

1996 ◽

Vol 3 (3) ◽

pp. 143-147 ◽

Cited By ~ 4

Author(s):

Ernesto G. Scerpella ◽

John J. Mathewson ◽

Herbert L. DuPont ◽

Francisco G. Martinez-Sandoval ◽

David N. Taylor ◽

...

Keyword(s):

North American ◽

Antibody Responses ◽

Cholera Vaccine ◽

Whole Cell ◽

B Subunit ◽

Oral Cholera Vaccine

Download Full-text

Total and Specific Immunoglobulin E and Their Relationship to Respiratory Symptoms in Quebec Children and Adolescents

Canadian Respiratory Journal ◽

10.1155/2005/248714 ◽

2005 ◽

Vol 12 (8) ◽

pp. 426-432 ◽

Cited By ~ 6

Author(s):

Benoît Levesque ◽

Jean-François Duchesne ◽

Suzanne Gingras ◽

Pierre Allard ◽

Edgar Delvin ◽

...

Keyword(s):

Children And Adolescents ◽

Respiratory Symptoms ◽

Immunoglobulin E ◽

Geometric Mean ◽

Allergic Sensitization ◽

Population Sample ◽

Ethnic Origin ◽

Total Ige ◽

Specific Immunoglobulin E ◽

Specific Immunoglobulin

BACKGROUND: Respiratory disease is a major cause of morbidity in young people. It is now recognized that atopy plays an important role in the development of chronic respiratory symptoms in children.OBJECTIVE: To examine the determinants and consequences of serum total and specific immunoglobulin E (IgE) in a general population sample of Québec children and adolescents.METHODS: In 1999, 2349 children and adolescents (nine, 13 or 16 years of age) who had participated in a respiratory symptom and disease questionnaire had their total IgE measured. Of these participants, a subsample of 451 children and adolescents was analyzed to detect antibodies to eight specific allergens (ie, allergens of dust mites [Dermatophagoides farinae and Dermatophagoides pteronyssinus], cat, dog, ragweed, Timothy grass, mould [alternaria] and cockroach).RESULTS: The geometric mean of the total IgE was 44.4 U/mL among all participants. Concentrations were higher in boys and increased with age. More than 41% of the participants were sensitized to at least one specific allergen. Such sensitization was strongly associated with the occurrence of respiratory conditions and symptoms, namely asthma, wheezing and rhinitis. Family history, school location and ethnic origin had an impact on the prevalence of atopy and total IgE levels.CONCLUSIONS: Allergic sensitization is a major determinant in the development of asthma, wheezing and rhinitis in children and adolescents in the province of Québec.

Download Full-text

Lack of evidence of IgE allergic sensitisation from working with lactic acid bacteria in the dairy foods industry

Journal of Dairy Research ◽

10.1017/s0022029918000419 ◽

2018 ◽

Vol 85 (3) ◽

pp. 355-357

Author(s):

Coralie Barrera ◽

Gabriel Reboux ◽

Audrey Laboissière ◽

Laurence Millon ◽

Anne Oppliger

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Preventive Measures ◽

Immunoglobulin E ◽

Milk Powder ◽

Medical Practitioner ◽

Specific Ige ◽

Adverse Health Effects ◽

High Concentrations ◽

Ige Response

This research communication aimed to evaluate the level of immunoglobulin E from lactic acid bacteria (LAB) that are used in dairy industries. Previous studies have demonstrated that workers report symptoms of irritation and are frequently IgG-sensitised to LAB. Workers (n = 44) from a probiotic production unity and the control lab were seen by a medical practitioner and responded to an occupational questionnaire. Specific IgE by the DELFIA® technique against 6 strains of LAB were measured on 44 exposed workers and 31 controls sera. Levels of specific IgE were low and no difference was observed between the two groups. This lack of IgE response could be explained by a healthy worker effect, an efficient implementation of personal protective equipment or by an absence of allergic mechanisms to account for the self-reported irritative symptoms. Despite the high concentrations of LAB, preventive measures are effective enough to guarantee no allergic effect and to prevent other adverse health effects. The implementation of preventive measures to avoid or reduce exposure to dust of LAB, and more generally to milk powder, is recommended in all dairy industry.

Download Full-text

Proficiency Survey-Based Evaluation of Clinical Total and Allergen-Specific IgE Assay Performance

Archives of Pathology & Laboratory Medicine ◽

10.5858/2009-0518-oa.1 ◽

2010 ◽

Vol 134 (7) ◽

pp. 975-982 ◽

Cited By ~ 9

Author(s):

Robert G. Hamilton

Keyword(s):

Allergic Disease ◽

Immunoglobulin E ◽

Diagnostic Algorithm ◽

Specific Ige ◽

Total Serum ◽

Design Data ◽

Ige Antibody ◽

Marked Variability ◽

Specific Immunoglobulin E ◽

History Of

Abstract Context.—The diagnostic algorithm for human allergic disease involves confirmation of sensitization by detection of allergen-specific immunoglobulin E (IgE) antibody in individuals suspected of having allergic disease because of a history of allergic symptoms after known allergen exposure. Previous studies showed wide disparity among clinically reported allergen-specific IgE levels from different serologic assays. Objective.—To validate the relative analytic performance (sensitivity, interassay reproducibility, linearity/parallelism, intermethod agreement) of clinically used total and allergen-specific IgE assays by using College of American Pathologists' Diagnostic Allergy “SE” Proficiency Survey data. Design.—Data from 2 SE survey cycles were used to assess relative analytic performance of the ImmunoCAP (Phadia), Immulite (Siemens Healthcare-Diagnostics), and HYTEC 288 (HYCOR-Agilent Technologies) total and allergen-specific IgE assays. In each cycle, 2 recalcified plasma pools from atopic donors were diluted twice with IgE-negative serum and evaluated in approximately 200 federally certified clinical laboratories for total IgE and IgE antibody to 5 allergen specificities. Statistical analysis evaluated analytic sensitivity, linearity, reproducibility, and intermethod agreement. Results.—Interlaboratory intramethod, intermethod, and interdilution agreement of all 6 clinically used total serum IgE assays were excellent, with coefficients of variation (CVs) below 15%. Interlaboratory intramethod, and interdilution agreement of 3 clinically used allergen-specific IgE assays were also excellent with CVs below 15%. However, intermethod CVs identified between-assay disagreement greater than 20% in 80% of allergen-specific IgE measurements. Allergen reagents and patients' immune response heterogeneity are suggested probable causes. Conclusions.—Clinical total and allergen-specific IgE assays display excellent analytic sensitivity, precision, reproducibility, and linearity. Marked variability in quantitative estimates of allergen-specific IgE from clinically used automated immunoassays is a concern that may be ameliorated with component allergen use.

Download Full-text