Ationship between ultrasonic characteristics of the corpus luteum, plasma progesterone concentration and early pregnancy diagnosis in friesian mares

1999 ◽  
Vol 52 (4) ◽  
pp. 585-592 ◽  
Author(s):  
M Sevinga ◽  
Y.H. Schukken ◽  
J.W. Hesselink ◽  
F.H Jonker
2009 ◽  
Vol 21 (1) ◽  
pp. 254
Author(s):  
A. Gaja ◽  
C. Kubota ◽  
T. Kojima

The present study aims to establish a novel practical protocol for early pregnancy diagnosis in cows by using transrectal ultrasonography. The protocol is based on measurements of corpus luteum (CL) cross-sectional area (CL c-s area) change performed at 2 separate days before the coming estrus after AI. Fourteen cows were inseminated artificially, and transrectal ultrasonographical observation of the ovaries and blood collection for measurement of peripheral plasma progesterone (P4) concentration were carried out daily from Days 12 to 23 (Day 0 = the day of onset of estrus). Thereafter, cows were routinely diagnosed for pregnancy at Day 30 by transrectal ultrasonography. The largest CL c-s area was obtained at Day 14 in both pregnant and non-pregnant cows. Seven out of 8 non-pregnant cows showed significant CL c-s area regression between Days 14 and 20 (422 ± 112 v. 249 ± 63 mm2), whereas no regression was observed between Days 14 and 20 in pregnant cows (416 ± 65 v. 402 ± 78 mm2). The regression in the CL c-s area between pregnant and non-pregnant cows was significantly different during Day 18 (424 ± 65 v. 288 ± 88 mm2) to Day 23 (402 ± 71 v. 139 ± 64 mm2). P4 concentration was significantly low (less than 1 ng mL–1) at Day 20 in 3 out of 8 non-pregnant cows, whereas the pregnant cows showed significant increase of P4 between Days 14 and 20 (2.6 ± 0.2 v. 3.4 ± 0.5 ng mL–1). The pregnant cows showed significantly higher P4 concentration starting from Day 18 than non-pregnant cows. However, in non-pregnant cows, 4 cows returned to estrus on Day 20 or after, 3 cows showed no signs of estrus, and 1 cow came in estrus as early as Day 18 after AI. In conclusion, the results of the present study suggest that measuring the change in the CL c-s area at Days 14 and 20 makes it possible to detect the non-pregnant cows at Day 20 after AI. However, it was also indicated that measuring the change of P4 concentrations on the same days did not always successfully detect non-pregnant cows. The new protocol based on CL c-s area regression rate can detect almost certainly non-pregnant cows at Day 20 after AI. It is suggested that this method is advantageous in research and industrial breeding.


1980 ◽  
Vol 106 (5) ◽  
pp. 104-106 ◽  
Author(s):  
B. Perera ◽  
N. Pathiraja ◽  
S. Abeywardena ◽  
M. Motha ◽  
H. Abeygunawardena

1999 ◽  
Vol 42 (2) ◽  
pp. 183-190
Author(s):  
F. Rehbock ◽  
F. Schneider ◽  
S. Zupp ◽  
S. Grumbach

Abstract. Title of the paper: Seasonal influences on blood plasma progesterone concentration in ewes of Black head Mutton sheep breed (short communication) In 71 Black head mutton sheeps on pasturing and housing were determined from the year 1995 to 1997 and there in the period from March to October the concentration of blood progesterone for determination of onset of estrus. The level of blood progesterone reported, on literature also, no cycle on this period. The investigation of 6 of this sheeps with ultrasonography had the result of ovarian activity with follicles from 2 mm to 7 mm in diameter, but no cycle was determined. The progesterone level of minimum and maximum on all years differed from March to beginning of September between 0,33 ng/ml and 2,15 ng/ml blood progesterone, after this to October between 0,82 ng/ml and 4,25 ng/ml blood progesterone. The pregnant sheeps detected blood progesterone concentrations from 5,50 ng/ml to 13,55 ng/ml blood progesterone (twins). The corpus luteum activity may be is prevalent on the blood progesterone level up 2 ng/ml and pregnancy up 5 ng/ml blood progesterone. The detectability of blood progesterone and supplemented ultrasonography of ovary possess the possibility recording of onset cycle activity and early pregnancy diagnosis.


1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.


1972 ◽  
Vol 52 (4) ◽  
pp. 645-658 ◽  
Author(s):  
H. A. ROBERTSON

The progesterone concentration in peripheral plasma has been measured sequentially in individual cows during the estrous cycle, gestation, at parturition, and post-partum. During the estrous cycle the concentration was lowest just before, during, and just after estrus when the level (0.1–0.4 ng/ml) was similar to that found in three ovariectomized cows. The concentration commenced to rise on the 4th–6th day (day of estrus = 1st day), reached a peak of 3–6 ng/ml on the 11th–13th day, and dropped rapidly over a 24–48-hr period to a basal value 24–72 hr before the next estrus. In early pregnancy, the plasma progesterone concentration was similar to the maximum levels found during the luteal phase of the estrous cycle. Between 90 and 150 days there was an indication of a decline to a relatively low plasma progesterone concentration followed by a variable rise. Prior to parturition, there was a gradual decline over a period of 35–70 days reaching a level of <2.0 ng/ml the day before parturition. Following parturition, the level remained at <0.5 ng/ml until the first sign of the resumption of cyclic activity. The time for this to occur was very variable (20–>60 days). Although not conclusive, the evidence favors the view that for estrous behavior to occur, estrus must be preceded by luteal activity. The discharge of mucus can occur without previous luteal activity.


2003 ◽  
Vol 2003 ◽  
pp. 1-1
Author(s):  
B.V.E. Segwagwe ◽  
K.L. Macmillan ◽  
P.D. Mansell

Oestrous synchronisation involves synchronisation of ovarian follicular turnover, new wave emergence, and finally induction of ovulation which can be achieved with an injection of either GnRH (Pursley et al. 1997)or oestradiol benzoate (ODB) (Day et al. 2000). A comparative study investigating corpus luteum (CL) and follicular emergence after the administration of either GnRH or ODB at pro-oestrus has not been reported. It was hypothesised that the injection of ODB at pro-oestrus would delay emergence of the first post-ovulatory follicular wave, but that CL development and plasma progesterone concentrations would be similar in cows induced to ovulate with either GnRH or ODB.


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