Characterization of total membrane proteins of Porphyromonas endodontalis

1992 ◽  
Vol 18 (12) ◽  
pp. 620-624 ◽  
Author(s):  
Jantien A. Herweijer ◽  
Bruno G. Loos ◽  
Mirdza E. Neiders
Keyword(s):  
Membrane Proteins ◽  
Total Membrane

Membranes of chromaffin granules. Isolation and partial characterization of two proteins

1971 ◽  
Vol 122 (3) ◽  
pp. 298.1-304 ◽  
Author(s):  
Heide Hörtnagl ◽  
H. Winkler ◽  
J. A. L. Schöpf ◽  
W. Hohenwallner
Keyword(s):  
Membrane Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Major Protein ◽  
Chromaffin Granules ◽  
Protein Components ◽  
Acid Gel ◽  
Total Membrane

Membranes of chromaffin granules were isolated from the adrenal glands of four different species. The solubilized membrane proteins could be resolved into several bands by polyacrylamide-gel electrophoresis (alkaline and acid gel systems). Two major protein components appeared to be common to the chromaffin granule membranes of ox, horse, pig and man. The various membrane proteins of bovine chromaffin granules were separated by filtration on Sephadex G-200 in the presence of sodium dodecyl sulphate. Two major membrane proteins (A and B) were obtained in purified form. Treatment of protein A with 2-mercaptoethanol before electrophoresis resulted in two more rapidly migrating subunits, whereas protein B was unaffected by mercaptoethanol treatment. The amino acid compositions of the two purified proteins were determined. They are very similar to that of the total membrane proteins but significantly different from that of the chromogranins, the soluble proteins of chromaffin granules.

Characterization of PrP binding proteins

1994 ◽  
Vol 343 (1306) ◽  
pp. 443-445 ◽  
Keyword(s):  
Membrane Proteins ◽  
Prion Protein ◽  
Binding Proteins ◽  
Mammalian Species ◽  
Integral Membrane Proteins ◽  
Infectious Particle ◽  
Spongiform Degeneration ◽  
The Brain ◽  
Total Membrane

Prions cause spongiform degeneration in various mammalian species. The scrapie prion protein (PrP Sc ) is part of the infectious particle and may mediate infection and spreading of the disease in the brain. It was therefore of interest to purify and analyse PrP ligands (Plis). Plis were identified on ligand blots using either intact PrP or peptides corresponding to the central portion of PrP. Here, characterization of a 110 and a 125 kDa Pli is reported. Both Plis were found in total membrane fractions and could be extracted with carbonate indicating that they are not integral membrane proteins. On sucrose gradients both PrP ligands sedimented with high density particles.

Characterization of microvillar membrane proteins of dog small intestine by two-dimensional electrophoresis

1995 ◽  
Vol 110 (3) ◽  
pp. 483-492 ◽  
Author(s):  
Philip W. Pemberton ◽  
Robert W. Lobley ◽  
Raymond Holmes ◽  
Susanne H. Sørensen ◽  
Kenneth W. Simpson ◽  
...  
Keyword(s):  
Small Intestine ◽  
Membrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Characterization of two integral membrane proteins located in the protein bodies of pumpkin seeds

1995 ◽  
Vol 28 (6) ◽  
pp. 1089-1101 ◽  
Author(s):  
Kaori Inoue ◽  
Yuka Takeuchi ◽  
Mikio Nishimura ◽  
Ikuko Hara-Nishimura
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Protein Bodies ◽  
Pumpkin Seeds

scholarly journals Characterization of Plasma Membrane Proteins in Mammalian Kidney

1969 ◽  
Vol 244 (13) ◽  
pp. 3561-3569
Author(s):  
D F Fitzpatrick ◽  
G R Davenport ◽  
L Forte ◽  
E J Landon
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins ◽  
Mammalian Kidney
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