Reparative dentin formation in rat molars after direct pulp capping with growth factors

1998 ◽  
Vol 24 (11) ◽  
pp. 744-751 ◽  
Author(s):  
C.-C. Hu ◽  
Chuhua Zhang ◽  
Qiubing Qian ◽  
Nanni B. Tatum
Keyword(s):  
Growth Factors ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Rat Molars ◽  
Reparative Dentin Formation

scholarly journals Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

2019 ◽  
Vol 12 (4) ◽  
pp. 182-186
Author(s):  
Mozammal Hossain ◽  
Mahmood Sajedeen ◽  
Yukio Nakamura
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Normal Function ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Exposure Site ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

Dentonin, a MEPE Fragment, Initiates Pulp-healing Response to Injury

2007 ◽  
Vol 86 (8) ◽  
pp. 780-785 ◽  
Author(s):  
N. Six ◽  
D. Septier ◽  
C. Chaussain-Miller ◽  
R. Blacher ◽  
P. DenBesten ◽  
...  
Keyword(s):  
Synthetic Peptide ◽  
Matrix Proteins ◽  
Dental Tissues ◽  
Agarose Beads ◽  
Reparative Dentin ◽  
Pulp Cells ◽  
Dentin Sialoprotein ◽  
Dentin Formation ◽  
Rat Molars ◽  
Reparative Dentin Formation

Phosphorylated extracellular matrix proteins, including matrix extracellular phosphoprotein (MEPE), are involved in the formation and mineralization of dental tissues. In this study, we evaluated the potential of Dentonin, a synthetic peptide derived from MEPE, to promote the formation of reparative dentin. Agarose beads, either soaked with Dentonin or unloaded, were implanted into the pulps of rat molars, and examined 8, 15, and 30 days after treatment. At day 8, Dentonin promoted the proliferation of pulp cells, as visualized by PCNA-labeling. RP59-positive osteoblast progenitors were located around the Dentonin-soaked beads. PCNA- and RP59-labeling were decreased at day 15, while osteopontin, weakly labeled at day 8, was increased at 15 days, but dentin sialoprotein was undetectable at any time. At 8 days, precocious reparative dentin formation occurred in pulps containing Dentonin-soaked beads, with formation slowing after 15 days. These results suggest that Dentonin affects primarily the initial cascade of events leading to pulp healing.

scholarly journals Reactionary and reparative dentin formation after pulp capping: Hydrogel vs. Dycal

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
A Njeh ◽  
E Uzunoğlu ◽  
H Ardila-Osorio ◽  
S Simon ◽  
A Berdal ◽  
...  
Keyword(s):  
Pulp Capping ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Dentin Regeneration in Vital Pulp Therapy: Design Principales

2001 ◽  
Vol 15 (1) ◽  
pp. 96-100 ◽  
Author(s):  
D. Tziafas ◽  
G. Belibasakis ◽  
A. Veis ◽  
S. Papadimitriou
Keyword(s):  
Artificial Substrates ◽  
Tissue Architecture ◽  
Pulp Capping ◽  
Pulp Therapy ◽  
Vital Pulp Therapy ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Direct Induction ◽  
Reparative Dentin Formation ◽  
Dentin Regeneration

The nature and specificity of the mechanisms by which the amputated dentin-pulp interface is therapeutically healed determine the properties of the barrier at this site and play a critical role in the outcome of vital pulp therapy. Healing of the dentin-pulp complex proceeds either by natural repair-which results in defensive hard-tissue formation, or therapeutically regulated dentin regeneration, which aims to reconstitute the normal tissue architecture at the pulp periphery. Progress in biomedical research opens new directions for the design of biologically effective pulp therapies. Application of biocompatible and biodegradable carrier vehicles for local delivery of signaling molecules in pulp-capping situations showed induction of fibrodentin/reparative dentin formation, but often at the expense of underlying pulp tissue. An alternative pre-clinical model aiming to reconstitute normal tissue architecture directly at the dentin-pulp interface should be designed on the basis of the direct induction of odontoblast-like cell differentiation and reparative dentin formation at the pulp-capping material interface. Experimental data clearly showed that pulpal cells can differentiate directly into odontoblast-like cells in association with specific extracellular matrices (dentinal or fibrodentinal matrix) or TGFβ1-containing artificial substrates. Dentin-induced dentinogenesis can be used as a master plan for the achievement of new therapeutic opportunities. In the present study, several short-term experimental studies on dog teeth for potential direct induction of odontoblast-like cell differentiation at the surface of rhTGFβ1-containing artificial substrates (Millipore filters, hydroxyapatite granules, calcium hydroxide, pure titanium) failed to induce any specific reparative dentinogenic effects.

scholarly journals Knowledge, attitude and practise of usage of MTA and bio dentine as direct pulp capping agents - A questionnaire study

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 744-756
Author(s):  
Sudarsan R ◽  
Balaji Ganesh S ◽  
Anjaneyulu K
Keyword(s):  
Calcium Hydroxide ◽  
Undergraduate Students ◽  
Direct Pulp Capping ◽  
Online Questionnaire ◽  
Capping Agents ◽  
Pulp Capping ◽  
Reparative Dentin ◽  
Study Population ◽  
Spss Software ◽  
Dentin Formation

Direct pulp-capping is a procedure in which exposed vital pulp is treated by placing a dental material over dental pulp to maintain vitality and to facilitate reparative dentin formation. The most commonly used pulp-capping material for decades is calcium hydroxide. But due to a number of disadvantages, there is a need for an alternate ideal pulp capping material. Recently MTA and Biodentine have been suggested as promising candidates for use in direct pulp capping procedures. The aim of the present study is to evaluate the knowledge, attitude and practise of usage of MTA and biodentine as direct pulp capping agents. An online questionnaire consisting of 15 questions which included demographic details, and questions regarding their knowledge and awareness of direct pulp therapy and the properties of MTA and biodentine. The study was conducted among undergraduate students of a private dental institute. Data was collected from filled questionnaires and analysed using SPSS software. Majority of the respondents (40.1%) chose calcium hydroxide as the material of choice for direct pulp capping and only 30% of the study population chose both MTA and biodentine as a direct pulp capping agent, which was statistically significant (p=0.000). 52% of the respondents were not aware of the major drawbacks of MTA. Knowledge regarding properties of biodentine in comparison to MTA was higher. Within the limits of the study, it shows that the knowledge in regard to the use of MTA and biodentine as direct pulp capping was poor despite recent studies showing a growing number of advantages over calcium hydroxide.

scholarly journals Effect of hydroxyapatite and growth factors on reparative dentin formation in the therapy of injured pulp

2019 ◽  
pp. 32-32
Author(s):  
Zorana Velickovic ◽  
Dusan Zivkovic ◽  
Marija Bubalo ◽  
Milan Zivkovic ◽  
Aleksandar Mitic ◽  
...  
Keyword(s):  
Growth Factors ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

scholarly journals Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2491
Author(s):  
Keita Ipposhi ◽  
Atsushi Tomokiyo ◽  
Taiga Ono ◽  
Kozue Yamashita ◽  
Muhammad Anas Alhasan ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Nodule Formation ◽  
Dental Pulp Cells ◽  
Related Protein ◽  
Direct Pulp Capping ◽  
Odontoblastic Differentiation ◽  
Reparative Dentin ◽  
Pulp Cells ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Direct pulp capping is an effective treatment for preserving dental pulp against carious or traumatic pulp exposure via the formation of protective reparative dentin by odontoblast-like cells. Reparative dentin formation can be stimulated by several signaling molecules; therefore, we investigated the effects of secreted frizzled-related protein (SFRP) 1 that was reported to be strongly expressed in odontoblasts of newborn molar tooth germs on odontoblastic differentiation and reparative dentin formation. In developing rat incisors, cells in the dental pulp, cervical loop, and inner enamel epithelium, as well as ameloblasts and preodontoblasts, weakly expressed Sfrp1; however, Sfrp1 was strongly expressed in mature odontoblasts. Human dental pulp cells (hDPCs) showed stronger expression of SFRP1 compared with periodontal ligament cells and gingival cells. SFRP1 knockdown in hDPCs abolished calcium chloride-induced mineralized nodule formation and odontoblast-related gene expression and decreased BMP-2 gene expression. Conversely, SFRP1 stimulation enhanced nodule formation and expression of BMP-2. Direct pulp capping treatment with SFRP1 induced the formation of a considerable amount of reparative dentin that has a structure similar to primary dentin. Our results indicate that SFRP1 is crucial for dentinogenesis and is important in promoting reparative dentin formation in response to injury.

Sign in / Sign up

Export Citation Format

Share Document