Overexpression of a serine alkaline protease gene in Bacillus licheniformis and its impact on the metabolic reaction network

2003 ◽  
Vol 32 (6) ◽  
pp. 706-720 ◽  
Author(s):  
Pınar Çalik ◽  
Gregory C. Tomlin ◽  
Stephen G. Oliver ◽  
Tunçer H. Özdamar
Keyword(s):  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Reaction Network ◽  
Protease Gene ◽  
Metabolic Reaction ◽  
Serine Alkaline Protease ◽  
Alkaline Protease Gene

scholarly journals Characterization and Optimization of Alkaline Protease Production from Bacillus licheniformis HSW-16 Isolated from Sambhar Salt Lake

2015 ◽  
Vol 3 (2) ◽  
pp. 347-351
Author(s):  
Rajnish Prakash Singh ◽  
Prabhat Nath Jha
Keyword(s):  
Bacillus Licheniformis ◽  
Alkaline Protease ◽  
Salt Lake ◽  
Industrial Applications ◽  
Protease Production ◽  
Protease Gene ◽  
Potential Source ◽  
Alkaline Protease Gene ◽  
Bacterium Bacillus ◽  
Ph Range

Halophilic microorganisms are recognized as potential source of secondary metabolites including enzymes and drugs with wide agricultural and industrial applications. In the present study protease producing halotolerant bacterium Bacillus licheniformis HSW-16 was isolated from hypersaline Sambhar lake, Rajasthan India. Protease production was performed by using azocasein as substrate. Confirmation of protease production was also done by amplification of alkaline protease gene and sequencing. The various nutritional factors such as carbon and nitrogen source and other physiological parameters like pH, temperature, incubation time and agitation speed were optimized for optimum protease production. The enzyme was active in pH range 7-10, temperature 25 °C-40 °C and salt concentration of 1.5M. The characteristics demonstrated by this isolate showed that it could be used as a potential source of enzyme.Int J Appl Sci Biotechnol, Vol 3(2): 347-351 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12757 

Using 16S rDNA as Target Site for Homologous Recombination to Improve the Alkaline Protease Production of Bacillus alcalophilus

2014 ◽  
Vol 886 ◽  
pp. 349-354
Author(s):  
Qing Shan Mo ◽  
Yao Tian ◽  
Hui Tu Zhang ◽  
Ling Jun Bu ◽  
Fu Ping Lu
Keyword(s):  
16S Rdna ◽  
Alkaline Protease ◽  
Genetic Manipulation ◽  
Protease Production ◽  
Protease Gene ◽  
Wild Type ◽  
Recombinant Strains ◽  
Manipulation System ◽  
Rdna Sites ◽  
Alkaline Protease Gene

Bacillus alcalophilusisolated was used for the production of alkaline protease. The enzyme encoded by alkaline protease gene (apr4) gene. To further improve the production of the strain for industrial requirement, a genetic manipulation system forBacillus alcalophiluswas developed. Additional copies of theapr4 gene were transferred into the strainBacillus alcalophilusand integrated into the 16S rDNA sites, yielding a series of recombinant strains. One of these recombinant strains, designated K23, exhibited superior properties for production of alkaline protease. the protease activity of K23 achieved by (6.19 ± 0.34) × 104U/ml, which is approximately 111.3% higher than that of the wild-type ones for 50-h fermentation. In addition, the new strain was genetically stable for more than 100 generations. These superior characteristics make it to be more suitable than the wild-type strain for alkaline protease production.

scholarly journals Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene.

1991 ◽  
Vol 57 (4) ◽  
pp. 901-909 ◽  
Author(s):  
J C van der Laan ◽  
G Gerritse ◽  
L J Mulleners ◽  
R A van der Hoek ◽  
W J Quax
Keyword(s):  
Alkaline Protease ◽  
Protease Gene ◽  
Chromosomal Integration ◽  
Alkaline Protease Gene
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