Allelic family specific humoral responses to merozoite surface protein 2 (MSP2) in Gabonese residents with Plasmodium falciparum infections

Abstract Background: The characterization of parasite populations circulating in malaria endemic areas is necessary to evaluate the success of ongoing interventions and malaria control strategies. This study was designed to investigate the genetic diversity of Plasmodium falciparum isolates from the semi-arid area in North East Ethiopia, using the highly polymorphic merozoite surface protein-2 (msp2) gene as a molecular marker. Methods: Dried blood spot isolates were collected from patients with Plasmodium falciparum infection between September 2014 and January 2015 from Melka-Werer, North East Ethiopia. Parasite DNA was extracted and genotyped using allele-specific nested polymerase chain reactions for msp2. Results: 52 isolates were collected with msp2 identified in 41 (78.8%) isolates. Allele typing of the msp2 gene detected the 3D7/IC allelic family in 54% and FC27 allelic family in 46%. A total of 14 different msp2 genotypes were detected including 6 belonging to the 3D7/IC family and 8 to the FC27 family. Forty percent of isolates had multiple genotypes and the overall mean multiplicity of infections (MOI) was 1.2 (95%CI 0.96-1.42). The heterozygosity index was 0.50 for the msp2 locus. There was no difference in MOI between age groups. A negative correlation between parasite density and multiplicity of infection was found (p = 0.02).Conclusion: P. falciparum isolates from the semi-arid area of North East Ethiopia are mainly monoclonal with low MOI and limited genetic diversity in the study population.

Download Full-text

Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker

10.21203/rs.3.rs-116121/v2 ◽

2021 ◽

Author(s):

Hussein Mohammed Ali ◽

Ashenafi Assefa ◽

Melkie Chernet ◽

Yonas Wulataw ◽

Robert J Commons

Keyword(s):

Genetic Diversity ◽

Plasmodium Falciparum ◽

Molecular Marker ◽

Surface Protein ◽

Merozoite Surface Protein ◽

Arid Area ◽

Chain Reactions ◽

Allelic Family ◽

North East ◽

Semi Arid

Abstract Background The characterization of parasite populations circulating in malaria endemic areas is necessary to evaluate the success of ongoing interventions and malaria control strategies. This study was designed to investigate the genetic diversity of Plasmodium falciparum isolates from the semi-arid area in North East Ethiopia, using the highly polymorphic merozoite surface protein-2 (msp2) gene as a molecular marker. Methods Dried blood spot isolates were collected from patients with P. falciparum infection between September 2014 and January 2015 from Melka-Werer, North East Ethiopia. Parasite DNA was extracted and genotyped using allele-specific nested polymerase chain reactions for msp2. Results 52 isolates were collected with msp2 identified in 41 (78.8%) isolates. Allele typing of the msp2 gene detected the 3D7/IC allelic family in 54% and FC27 allelic family in 46%. A total of 14 different msp2 genotypes were detected including 6 belonging to the 3D7/IC family and 8 to the FC27 family. Forty percent of isolates had multiple genotypes and the overall mean multiplicity of infections (MOI) was 1.2 (95%CI 0.96-1.42). The heterozygosity index was 0.50 for the msp2 locus. There was no difference in MOI between age groups. A negative correlation between parasite density and multiplicity of infection was found (p = 0.02).Conclusion Plasmodium falciparum isolates from the semi-arid area of North East Ethiopia are mainly monoclonal with low MOI and limited genetic diversity in the study population.

Download Full-text

Recombinant chimeric proteins generated from conserved regions of Plasmodium falciparum merozoite surface protein 2 generate antiparasite humoral responses in mice

Parasite Immunology ◽

10.1046/j.1365-3024.2000.00293.x ◽

2000 ◽

Vol 22 (5) ◽

pp. 211-221 ◽

Cited By ~ 10

Author(s):

Nicole Lawrence ◽

Anthony Stowers ◽

Victoria Mann ◽

Darrin Taylor ◽

Allan Saul

Keyword(s):

Plasmodium Falciparum ◽

Surface Protein ◽

Merozoite Surface Protein ◽

Chimeric Proteins ◽

Conserved Regions ◽

Falciparum Merozoite ◽

Humoral Responses

Download Full-text

Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker

Malaria Journal ◽

10.1186/s12936-021-03625-1 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Hussein Mohammed ◽

Ashenafi Assefa ◽

Melkie Chernet ◽

Yonas Wuletaw ◽

Robert J. Commons

Keyword(s):

Genetic Diversity ◽

Plasmodium Falciparum ◽

Molecular Marker ◽

Surface Protein ◽

Merozoite Surface Protein ◽

Arid Area ◽

Chain Reactions ◽

Allelic Family ◽

North East ◽

Semi Arid

AbstractBackgroundThe characterization of parasite populations circulating in malaria endemic areas is necessary to evaluate the success of ongoing interventions and malaria control strategies. This study was designed to investigate the genetic diversity ofPlasmodium falciparumisolates from the semi-arid area in North East Ethiopia, using the highly polymorphic merozoite surface protein-2 (msp2) gene as a molecular marker.MethodsDried blood spot isolates were collected from patients withP. falciparuminfection between September 2014 and January 2015 from Melka-Werer, North East Ethiopia. Parasite DNA was extracted and genotyped using allele-specific nested polymerase chain reactions formsp2.Results52 isolates were collected withmsp2identified in 41 (78.8%) isolates. Allele typing of themsp2gene detected the 3D7/IC allelic family in 54% and FC27 allelic family in 46%. A total of 14 differentmsp2genotypes were detected including 6 belonging to the 3D7/IC family and 8 to the FC27 family. Forty percent of isolates had multiple genotypes and the overall mean multiplicity of infections (MOI) was 1.2 (95%CI 0.96–1.42). The heterozygosity index was 0.50 for themsp2locus. There was no difference in MOI between age groups. A negative correlation between parasite density and multiplicity of infection was found (p = 0.02).ConclusionPlasmodium falciparumisolates from the semi-arid area of North East Ethiopia are mainly monoclonal with low MOI and limited genetic diversity in the study population.

Download Full-text

Plasmodium falciparum merozoite surface protein 1 (MSP1): genotyping and humoral responses to allele-specific variants

Acta Tropica ◽

10.1016/s0001-706x(01)00188-7 ◽

2002 ◽

Vol 81 (1) ◽

pp. 33-46 ◽

Cited By ~ 25

Author(s):

Marie-Thérèse Ekala ◽

Hélène Jouin ◽

Faustin Lekoulou ◽

Saadou Issifou ◽

Odile Mercereau-Puijalon ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Surface Protein ◽

Merozoite Surface Protein ◽

Merozoite Surface Protein 1 ◽

Falciparum Merozoite ◽

Allele Specific ◽

Humoral Responses

Download Full-text

Genetic polymorphism of Merozoite Surface Protein 1 (msp1) and 2 (msp2) genes and multiplicity of Plasmodium falciparum infection across various endemic areas in Senegal

African Health Sciences ◽

10.4314/ahs.v19i3.19 ◽

2019 ◽

Vol 19 (3) ◽

pp. 2446-2456

Author(s):

Tolla Ndiaye ◽

Mouhamad Sy ◽

Amy Gaye ◽

Daouda Ndiaye

Keyword(s):

Genetic Diversity ◽

Plasmodium Falciparum ◽

Surface Protein ◽

Merozoite Surface Protein ◽

Falciparum Infection ◽

Allelic Family ◽

Merozoite Surface Protein 1 ◽

Plasmodium Falciparum Infection ◽

Msp2 Gene ◽

Falciparum Population

Introduction: Despite a significant decline in Senegal, malaria remains a burden in various parts of the country. Assessment of multiplicity of Plasmodium falciparum infection and genetic diversity of parasites population could help in monitoring of malaria control.Objective: To assess genetic diversity and multiplicity of infection in P. falciparum isolates from three areas in Senegal with different malaria transmissions. Methods: 136 blood samples were collected from patients with uncomplicated P. falciparum malaria in Pikine, Kedougou and Thies. Polymorphic loci of msp1 and 2 (Merozoite surface protein-1 and 2) genes were amplified by nested PCR.Results: For msp1gene, K1 allelic family was predominant with frequency of 71%. Concerning msp2 gene, IC3D7 allelic family was the most represented with frequency of 83%. Multiclonal isolates found were 36% and 31% for msp1et msp2 genes respectively. The MOI found in all areas was 2.56 and was statistically different between areas (P=0.024). Low to intermediate genetic diversity were found with heterozygosity range (He=0,394-0,637) and low genetic differentiation (Fst msp1= 0.011; Fst msp2= 0.017) were observed between P. falciparum population within the country.Conclusion: Low to moderate genetic diversity of P.falciparum strains and MOI disparities were found in Senegal.Keywords: Senegal, MOI, Genetic diversity, msp1, msp2.

Download Full-text

Allelic diversity of merozoite surface protein genes (msp1 and msp2) and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia

Malaria Journal ◽

10.1186/s12936-021-03719-w ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Kurnia Fitri Jamil ◽

Nandha Rizki Pratama ◽

Sylvia Sance Marantina ◽

Harapan Harapan ◽

Muhammad Riza Kurniawan ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Liver Function ◽

Severe Malaria ◽

Clinical Manifestation ◽

Allelic Diversity ◽

Surface Protein ◽

Merozoite Surface Protein ◽

Malaria Control Programme ◽

Co Morbidity ◽

Aceh Province

Abstract Background The malaria control programme in Indonesia has successfully brought down malaria incidence in many parts in Indonesia, including Aceh Province. Clinical manifestation of reported malaria cases in Aceh varied widely from asymptomatic, mild uncomplicated to severe and fatal complications. The present study aims to explore the allelic diversity of merozoite surface protein 1 gene (msp1) and msp2 among the Plasmodium falciparum isolates in Aceh Province and to determine their potential correlation with the severity of malaria clinical manifestation. Methods Screening of over 500 malaria cases admitted to the hospitals in 11 districts hospital within Aceh Province during 2013–2015, identified 90 cases of P. falciparum mono-infection without any co-morbidity. The subjects were clinically phenotyped and parasite DNA was extracted and polymerase chain reaction (PCR) amplified for the msp1 and msp2 allelic subfamilies. Results Analysis of clinical manifestation revealed that fever-chill is the most frequent symptom. Based on WHO criteria showed 19 cases were classified as severe and 71 as mild malaria. Analysis of msp1 gene revealed the presence of K1 allele subfamily in 34 subjects, MAD20 in 42 subjects, RO33 in 1 subject, and mixed allelic of K1 + MAD20 in 5 subjects, K1 + RO33 in 4 subjects, and MAD20 + RO33 in 4 subjects. Analysis of msp2 gene revealed 34 subjects carried the FC27 allelic subfamily, 37 subjects carried the 3D7 and 19 subjects carried the mixed FC27 + 3D7. Analysis of multiplicity of infection revealed that msp1 alleles is slightly higher than msp2 with the mean of MOI were 2.69 and 2.27, respectively. Statistical analysis to determine the association between each clinical manifestation and msp1 and msp2 alleles revealed that liver function abnormal value was associated with the msp2 mixed alleles (odds ratio (OR):0.13; 95%CI: 0.03–0.53). Mixed msp1 of K1 + RO33 was associated with severe malaria (OR: 28.50; 95%CI: 1.59–1532.30). Conclusion This study found a strong association between severe malaria in Aceh with subjects carrying the msp1 mixed alleles of K1 and RO33. The liver function abnormal value associated with the msp2 mixed allelic subfamilies. Further study in different geographic areas is recommended.

Download Full-text