Detection of measles specific IgG in oral fluid using an FITC/anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA)

1999 ◽  
Vol 83 (1-2) ◽  
pp. 135-144 ◽  
Author(s):  
W Nigatu ◽  
D.J Nokes ◽  
F Enquselassie ◽  
D.W.G Brown ◽  
B.J Cohen ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Oral Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Igg

scholarly journals Rubella-specific IgG subclass concentrations in sera using an enzyme-linked immunosorbent assay (ELISA): the effect of different sources of rubella antigen

1988 ◽  
Vol 101 (3) ◽  
pp. 599-604 ◽  
Author(s):  
H. I. J Thomas ◽  
P. Morgan-Capner
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Subclass ◽  
Human Igg ◽  
Discrepant Result ◽  
Specific Igg ◽  
Antigen A ◽  
Different Sources ◽  
Positive Results

SUMMARYFive rubella antigens were evaluated in an antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG subclass antibody. One monoclonal anti-human IgG subclass antibody was used for each of IgG1, IgG2and IgG4, but two were compared for IgG3. A total of 101 sera were tested from cases of rubella in the distant past and from cases of primary rubella, reinfection and following immunization. Only one serum gave a discrepant result for specific IgG1, being positive with only one rubella antigen, a commercially prepared antigen coated on to microtitre wells (Enzygnost; Behringwerke). No sera contained detectable specific IgG2. Only four sera contained specific IgG4, and this was detectable only with Enzygnost antigen. For specific IgG3little difference was observed between the two monoclonal anti-human IgG3subclass antibodies; only two very weakly positive sera gave discrepant results. However, varying results were obtained for specific IgG3with the different antigens. Enzygnost gave more positive results for specific IgG3with most categories of sera.It is concluded that the differences between various reports of the rubella-specific IgG subclass profile cannot be explained entirely by the use of different rubella antigens.

scholarly journals Enzyme-Linked Immunosorbent Assay for Salmonella Serology using Lipopolysaccharide Antigen

1995 ◽  
Vol 7 (4) ◽  
pp. 481-487 ◽  
Author(s):  
Bradford P. Smith ◽  
George W. Dilling ◽  
John K. House ◽  
Hans Konrad ◽  
Nadia Moore
Keyword(s):  
Immunosorbent Assay ◽  
Epidemiologic Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Reactions ◽  
Vaccine Responses ◽  
O Antigen ◽  
Specific Igg ◽  
O Antigens ◽  
Somatic Antigens ◽  
Factor Antigen

Enzyme-linked immunosorbent assay (ELISA) using Salmonella lipopolysaccharide (LPS) to measure specific IgG titers in cattle has proven useful. Serology can be used to assess vaccine responses and infection rates, to detect carriers, and to aid in epidemiologic studies. The objective of this study was to assess cross-reactions using sera from cattle vaccinated with different Salmonella serogroups. ELISA plates using lipopolysaccharide from serogroup B, C1, C3, D1 or E1 as the plate antigens were tested. LPS was extracted from Salmonella typhimurium (Serogroup B; somatic antigens 01, 4, 12), S. montevideo (C1; 06, 7), S. kentucky (C3; 08, 20), S. dublin (D1; 01, 9, 12) and S. anatum (E1; 03, 10) using the Westphal method. Fifteen cows were found to be seronegative for all 5 of these serogroup antigens. Each cow was then vaccinated 3 times at 2-week intervals with a killed Salmonella bacterin. The 15 different serotypes used for vaccination were chosen to represent a wide array of Salmonella serogroups with a wide array of somatic “O” antigens expressed, including somatic antigens 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 18, 19, 20, 22, 23, 25, and 27. With each antigen tested, the highest ELISA titers were seen with sera from cattle vaccinated with homologous O antigens, indicating that reactions were highly O antigen-specific. Some cross reactions between serogroups sharing one O factor antigen were found; these titers were lower than those found with homologous serogroups sharing 2 or more antigens. Only serum from the cow vaccinated with S. anatum (group E; antigens 03, 10) cross-reacted at a low titer with group C1 (O somatic antigens 6, 7) and D1 (O somatic antigens 1, 9, 12) plate antigens, with which no somatic antigens were shared. We conclude from these results that Salmonella serology using LPS antigens is highly O antigen-specific and predictable.

scholarly journals Detection of the acute phase of abdominal angiostrongyliasis with a parasite-specific IgG enzyme linked immunosorbent assay

2001 ◽  
Vol 96 (4) ◽  
pp. 515-518 ◽  
Author(s):  
Stefan Michael Geiger ◽  
Antônio Carlo Laitano ◽  
Charlotte Sievers-Tostes ◽  
Aventino Alfredo Agostini ◽  
Hartwig Schulz-Key ◽  
...  
Keyword(s):  
Acute Phase ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Igg

scholarly journals Specific IgE response in patients with brucellosis

1990 ◽  
Vol 105 (3) ◽  
pp. 571-577 ◽  
Author(s):  
G. F. Araj ◽  
A. R. Lulu ◽  
M. I. Khateeb ◽  
M. Haj
Keyword(s):  
Immunosorbent Assay ◽  
Specific Ige ◽  
Blood Cultures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serological Markers ◽  
Microagglutination Test ◽  
Specific Igg ◽  
Ige Response

SUMMARYIn the search to find discriminative serological markers to differentiate between patients with acute brucellosis and those with chronic brucellosis, an enzyme-linked immunosorbent assay (ELISA) was used to determine and compare the brucella-specific IgE response in 80 sera from patients with acute brucellosis, 37 sera from patients with chronic brucellosis, 26 sera from patients with positive blood cultures for bacteria other than brucella and 51 sera from healthy controls. The IgE findings were compared to brucella-specific IgG, IgM, IgA and IgG1–4demonstrated by ELISA, and to microagglutination test (MAT) results. Elevated (positive) antibrucella IgE titres were detected in 89 and 81 % of sera from patients with acute and chronic brucellosis respectively. The predominant antibodies found in patients with acute brucellosis were of the IgG, IgM, IgA, IgE, IgG1and IgG3types while in chronic brucellosis IgG, IgA, IgE and IgG4were found. Although IgE can be detected in patients with brucellosis, it does not discriminate between the acute and chronic stages of the disease.

scholarly journals Comparison of Capture Immunoglobulin M (IgM) and IgG Enzyme-Linked Immunosorbent Assay (ELISA) and Nonstructural Protein NS1 Serotype-Specific IgG ELISA for Differentiation of Primary and Secondary Dengue Virus Infections

2003 ◽  
Vol 10 (4) ◽  
pp. 622-630 ◽  
Author(s):  
Pei-Yun Shu ◽  
Li-Kuang Chen ◽  
Shu-Fen Chang ◽  
Yi-Yun Yueh ◽  
Ling Chow ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Immunosorbent Assay ◽  
Primary Infection ◽  
Nonstructural Protein ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Igg Antibody ◽  
Previous Infection ◽  
Specific Igg

ABSTRACT We have found that NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) can be used to differentiate primary and secondary dengue virus infections. This is due to the fact that the NS1-specific IgG antibody cannot be detected before day 9 of illness for primary infection, so the NS1-specific IgG antibodies measured in acute-phase sera must come from previous infection. Comparison of NS1 serotype-specific IgG ELISA with envelope- and membrane-specific capture IgM and IgG ELISA in the differentiation of primary and secondary dengue virus infections showed good correlation (95.90% agreement). Most important, we have found that the serotype of the dengue virus from the majority of patients with primary infection could be correctly identified when convalescent-phase or postinfection sera were analyzed by NS1 serotype-specific IgG ELISA. These findings suggested that NS1 serotype-specific IgG ELISA could be reliably applied for serodiagnosis and seroepidemiological study of dengue virus infection.

scholarly journals Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

1988 ◽  
Vol 101 (3) ◽  
pp. 591-598 ◽  
Author(s):  
H. I. J. Thomas ◽  
P. Morgan-Capner
Keyword(s):  
Optical Density ◽  
Immunosorbent Assay ◽  
Primary Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Subclass ◽  
Antibody Avidity ◽  
Specific Antibodies ◽  
Specific Igg ◽  
Protein Denaturant

SUMMARYAn antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG1and IgG3was adapted to measure antibody avidity by incorporating a mild protein denaturant, diethylamine (DEA), into the serum diluent. Sera were tested at varying dilutions, both with and without DEA, if they contained sufficient specific IgG1or IgG3. The optical density (OD) was measured and curves were plotted. The highest OD (V) was noted and halved (V/2). The distance between the OD curves at V/2 was measured as the DEA shift value.Sera were examined from people whose sera contained rubella-specific antibodies as a consequence of infection or vaccination in the distant past (24 sera), recent primary rubella (66 sera), symptomatic reinfection (11 sera) or asymptomatic reinfection (64 sera). For specific IgG1the DEA shift value was <O·6 for cases of rubella in the distant past, compared with >0·8 for the first month after primary infection. The maximum DEA shift value for the sera from cases of reinfection was 0·65.No serum from cases of rubella in the distant past contained sufficient specific IgG3to estimate avidity. The sera collected within 1 month of onset of primary rubella gave DEA shift values >O·7 compared with sera from reinfections, which gave DEA shift values <O·6, except for two sera from a case of symptomatic reinfection.Thus the assessment of specific IgG subclass avidity is of value in differentiating serologically primary rubella from reinfection.

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