Quantitative assay of Salmonella adherence to intestinal epithelial cells: A new method for assessing novel intervention products

1998 ◽  
Vol 33 (2) ◽  
pp. 163-170 ◽  
Author(s):  
A Alwan ◽  
T Deignan ◽  
M O'Sullivan ◽  
J Kelly ◽  
C O'Farrelly
1966 ◽  
Vol 44 (6) ◽  
pp. 687-693 ◽  
Author(s):  
A. D. Perris

A new method for the preparation of isolated intestinal epithelial cells from the rat is described. Suspensions of these cells respire actively, taking up oxygen in a linear relationship with time for about 40 minutes. When actively transported sugars are present in the incubation medium, the cells utilize more oxygen. It is suggested that such preparations may be useful in the study of intestinal function at the cellular level.


Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.


2001 ◽  
Vol 120 (5) ◽  
pp. A504-A504
Author(s):  
A NEUMANN ◽  
M DEPKAPRONDZINSKI ◽  
C WILHELM ◽  
K FELGENHAUER ◽  
T CASPRITZ ◽  
...  

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