Fos expression in serotonergic midbrain neurons projecting to the paraventricular nucleus of hypothalamus after noxious stimulation of the stomach: a triple labeling study in the rat

1997 ◽  
Vol 27 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Yuan-Xiang Dong ◽  
Zhi-An Han ◽  
Kang-Hui Xiong ◽  
Zhi-Ren Rao
Keyword(s):  
Paraventricular Nucleus ◽  
Noxious Stimulation ◽  
Midbrain Neurons ◽  
Fos Expression ◽  
Paraventricular Nucleus Of Hypothalamus ◽  
Stimulation Of

ELECTROACUPUNCTURE DECREASES C-FOS EXPRESSION IN THE SPINAL CORD INDUCED BY NOXIOUS STIMULATION OF THE RAT BLADDER

1998 ◽  
pp. 2274-2279 ◽  
Author(s):  
CARL J. CHANG ◽  
SHIH-TSUNG HUANG ◽  
KENNETH HSU ◽  
AUSTIN LIN ◽  
MARSHALL L. STOLLER ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Noxious Stimulation ◽  
Rat Bladder ◽  
Fos Expression ◽  
Stimulation Of

Bombesin, but not amylin, blocks the orexigenic effect of peripheral ghrelin

2006 ◽  
Vol 291 (4) ◽  
pp. R903-R913 ◽  
Author(s):  
Peter Kobelt ◽  
Miriam Goebel ◽  
Andreas Stengel ◽  
Marco Schmidtmann ◽  
Ivo R. van der Voort ◽  
...  
Keyword(s):  
Food Intake ◽  
Neuronal Activity ◽  
Paraventricular Nucleus ◽  
Arcuate Nucleus ◽  
Vehicle Group ◽  
Fos Expression ◽  
Crf Neurons ◽  
Stimulation Of

The interaction between ghrelin and bombesin or amylin administered intraperitoneally on food intake and brain neuronal activity was assessed by Fos-like immunoreactivity (FLI) in nonfasted rats. Ghrelin (13 μg/kg ip) increased food intake compared with the vehicle group when measured at 30 min (g/kg: 3.66 ± 0.80 vs. 1.68 ± 0.42, P < 0.0087). Bombesin (8 μg/kg) injected intraperitoneally with ghrelin (13 μg/kg) blocked the orexigenic effect of ghrelin (1.18 ± 0.41 g/kg, P < 0.0002). Bombesin alone (4 and 8 μg/kg ip) exerted a dose-related nonsignificant reduction of food intake (g/kg: 1.08 ± 0.44, P > 0.45 and 0.55 ± 0.34, P > 0.16, respectively). By contrast, ghrelin-induced stimulation of food intake (g/kg: 3.96 ± 0.56 g/kg vs. vehicle 0.82 ± 0.59, P < 0.004) was not altered by amylin (1 and 5 μg/kg ip) (g/kg: 4.37 ± 1.12, P > 0.69, and 3.01 ± 0.78, respectively, P > 0.37). Ghrelin increased the number of FLI-positive neurons/section in the arcuate nucleus (ARC) compared with vehicle (median: 42 vs. 19, P < 0.008). Bombesin alone (4 and 8 μg/kg ip) did not induce FLI neurons in the paraventricular nucleus of the hypothalamus (PVN) and coadministered with ghrelin did not alter ghrelin-induced FLI in the ARC. However, bombesin (8 μg/kg) with ghrelin significantly increased neuronal activity in the PVN approximately threefold compared with vehicle and ∼1.5-fold compared with the ghrelin group. Bombesin (8 μg/kg) with ghrelin injected intraperitoneally induced Fos expression in 22.4 ± 0.8% of CRF-immunoreactive neurons in the PVN. These results suggest that peripheral bombesin, unlike amylin, inhibits peripheral ghrelin induced food intake and enhances activation of CRF neurons in the PVN.

scholarly journals Contribution of dorsal horn CGRP-expressing interneurons to mechanical sensitivity

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Line S Löken ◽  
Joao M Braz ◽  
Alexander Etlin ◽  
Mahsa Sadeghi ◽  
Mollie Bernstein ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Nerve Injury ◽  
Spinal Cord Dorsal Horn ◽  
Noxious Stimulation ◽  
Mechanical Sensitivity ◽  
Distinct Population ◽  
Calcitonin Gene ◽  
Spinal Cord Dorsal ◽  
Fos Expression ◽  
Stimulation Of

Primary sensory neurons are generally considered the only source of dorsal horn calcitonin gene-related peptide (CGRP), a neuropeptide critical to the transmission of pain messages. Using a tamoxifen-inducible CalcaCreER transgenic mouse, here we identified a distinct population of CGRP-expressing excitatory interneurons in lamina III of the spinal cord dorsal horn and trigeminal nucleus caudalis. These interneurons have spine-laden, dorsally-directed, dendrites and ventrally-directed axons. As under resting conditions, CGRP interneurons are under tonic inhibitory control, neither innocuous nor noxious stimulation provoked significant Fos expression in these neurons. However, synchronous, electrical non-nociceptive Aβ primary afferent stimulation of dorsal roots depolarized the CGRP interneurons, consistent with their receipt of a VGLUT1 innervation. On the other hand, chemogenetic activation of the neurons produced a mechanical hypersensitivity in response to von Frey stimulation whereas their caspase-mediated ablation led to mechanical hyposensitivity. Finally, after partial peripheral nerve injury, innocuous stimulation (brush) induced significant Fos expression in the CGRP interneurons. These findings suggest that CGRP interneurons become hyperexcitable and contribute either to ascending circuits originating in deep dorsal horn or to the reflex circuits in baseline conditions, but not in the setting of nerve injury.

ELECTROACUPUNCTURE DECREASES C-FOS EXPRESSION IN THE SPINAL CORD INDUCED BY NOXIOUS STIMULATION OF THE RAT BLADDER

1998 ◽  
Vol 160 (6 Part 1) ◽  
pp. 2274-2279 ◽  
Author(s):  
CARL J. CHANG ◽  
SHIH-TSUNG HUANG ◽  
KENNETH HSU ◽  
AUSTIN LIN ◽  
MARSHALL L. STOLLER ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Noxious Stimulation ◽  
Rat Bladder ◽  
Fos Expression ◽  
Stimulation Of

scholarly journals Submaximal exercise‐induced increase of c‐Fos expression in the paraventricular nucleus of hypothalamus is related to the body heating rate

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Juliana Bohnen Guimaraes ◽  
Gisele Vieira Rodovalho ◽  
Samuel Penna Wanner ◽  
Kênia Fiaux Nascimento ◽  
Cândido Celso Coimbra
Keyword(s):  
Heating Rate ◽  
Paraventricular Nucleus ◽  
Submaximal Exercise ◽  
The Body ◽  
Fos Expression ◽  
Exercise Induced ◽  
Paraventricular Nucleus Of Hypothalamus

Drastic Decrease in Isoflurane Minimum Alveolar Concentration and Limb Movement Forces after Thoracic Spinal Cooling and Chronic Spinal Transection in Rats

2005 ◽  
Vol 102 (3) ◽  
pp. 624-632 ◽  
Author(s):  
Steven L. Jinks ◽  
Carmen L. Dominguez ◽  
Joseph F. Antognini
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Minimum Alveolar Concentration ◽  
Tail Flick ◽  
Noxious Stimulation ◽  
Partial Recovery ◽  
Spinal Transection ◽  
Thoracic Spinal ◽  
Cord Injury ◽  
Stimulation Of

Background Individuals with spinal cord injury may undergo multiple surgical procedures; however, it is not clear how spinal cord injury affects anesthetic requirements and movement force under anesthesia during both acute and chronic stages of the injury. Methods The authors determined the isoflurane minimum alveolar concentration (MAC) necessary to block movement in response to supramaximal noxious stimulation, as well as tail-flick and hind paw withdrawal latencies, before and up to 28 days after thoracic spinal transection. Tail-flick and hind paw withdrawal latencies were measured in the awake state to test for the presence of spinal shock or hyperreflexia. The authors measured limb forces elicited by noxious mechanical stimulation of a paw or the tail at 28 days after transection. Limb force experiments were also conducted in other animals that received a reversible spinal conduction block by cooling the spinal cord at the level of the eighth thoracic vertebra. Results A large decrease in MAC (to &lt;/= 40% of pretransection values) occurred after spinal transection, with partial recovery (to approximately 60% of control) at 14-28 days after transection. Awake tail-flick and hind paw withdrawal latencies were facilitated or unchanged, whereas reflex latencies under isoflurane were depressed or absent. However, at 80-90% of MAC, noxious stimulation of the hind paw elicited ipsilateral limb withdrawals in all animals. Hind limb forces were reduced (by &gt;/= 90%) in both chronic and acute cold-block spinal animals. Conclusions The immobilizing potency of isoflurane increases substantially after spinal transection, despite the absence of a baseline motor depression, or "spinal shock." Therefore, isoflurane MAC is determined by a spinal depressant action, possibly counteracted by a supraspinal facilitatory action. The partial recovery in MAC at later time points suggests that neuronal plasticity after spinal cord injury influences anesthetic requirements.

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