Microgradients in bacterial colonies: use of fluorescence ratio imaging, a non-invasive technique.

2000 ◽  
Vol 56 (1) ◽  
pp. 71-80 ◽  
Author(s):  
P.K Malakar ◽  
T.F Brocklehurst ◽  
A.R Mackie ◽  
P.D.G Wilson ◽  
M.H Zwietering ◽  
...  
Keyword(s):  
Invasive Technique ◽  
Fluorescence Ratio ◽  
Non Invasive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging

scholarly journals Osmotic Stress Leads to Decreased Intracellular pH of Listeria monocytogenes as Determined by Fluorescence Ratio-Imaging Microscopy

2004 ◽  
Vol 70 (5) ◽  
pp. 3176-3179 ◽  
Author(s):  
Weihuan Fang ◽  
Henrik Siegumfeldt ◽  
Birgitte Bjørn Budde ◽  
Mogens Jakobsen
Keyword(s):  
Listeria Monocytogenes ◽  
Osmotic Stress ◽  
Liquid Medium ◽  
Intracellular Ph ◽  
Solid Medium ◽  
Fluorescence Ratio ◽  
Solid Media ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging

ABSTRACT Intracellular pH (pHi) of Listeria monocytogenes was determined after exposure to NaCl or sorbitol in liquid and solid media (agar). Both compounds decreased pHi, and recovery on solid medium was impaired compared to that in liquid medium. N,N′-dicyclohexylcarbodiimide abolished pHi recovery, and lowering aw with glycerol showed no effect on pHi.

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