Genomic organization of the rat Smad7 gene: Identification and functional characterization of the Smad binding element responsible for negative feedback regulation of TGFβ signaling

2000 ◽  
Vol 32 ◽  
pp. 148
Author(s):  
S. Dooley ◽  
M. Stopa ◽  
D. Anhuf ◽  
L. Terstegen ◽  
P. Gatsios ◽  
...  
Keyword(s):  
Negative Feedback ◽  
Genomic Organization ◽  
Functional Characterization ◽  
Feedback Regulation ◽  
Gene Identification ◽  
Tgfβ Signaling ◽  
Negative Feedback Regulation ◽  
Smad Binding Element

Functional and molecular characterization of luminal and basolateral Cl−/ HCO 3 − exchangers of rat thick limbs

1998 ◽  
Vol 275 (3) ◽  
pp. F334-F342 ◽  
Author(s):  
Dominique Eladari ◽  
Anne Blanchard ◽  
Françoise Leviel ◽  
Michel Paillard ◽  
Alan K. Stuart-Tilley ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Negative Feedback ◽  
Feedback Regulation ◽  
Membrane Vesicles ◽  
Thick Ascending Limb ◽  
Negative Feedback Regulation ◽  
Medullary Thick Ascending Limb ◽  
Internal Ph ◽  
External Ph

Cl−/[Formula: see text]exchange was measured in luminal (LMV) and basolateral (BLMV) membrane vesicles purified from rat medullary thick ascending limb (MTAL). Cl−/[Formula: see text]exchange in BLMV and LMV was inhibited by DIDS, with respective IC50 values of 3.2 ± 0.9 and 15.2 ± 5.2 μM, whereas Cl− conductances were DIDS insensitive. At constant external pH, BLMV36Cl−/[Formula: see text]and36Cl−/Cl−exchanges exhibited a sigmoidal pattern of activation as internal pH (pHi) increased from 6.1 to 8.0, whereas LMV36Cl−/Cl−exchange was unchanged between pHi6.7 and 7.8. The 165-kDa AE2 polypeptide and ∼115-kDa AE1-related polypeptide were present only in BLMV. In contrast, AE1-related polypeptides of ∼90 and 95 kDa were present not only in BLMV but also (in variable abundance) in LMV. We conclude that rat MTAL BLMV and LMV express distinct anion exchange activities and distinct sets of AE polypeptides. AE2 (and perhaps AE1) in BLMV likely contribute to[Formula: see text] absorption. In contrast, LMV exchangers may contribute to NaCl absorption via parallel coupling with the luminal Na+/H+antiporters and/or may provide negative feedback regulation of[Formula: see text] absorption.

scholarly journals The Structure of the PanD/PanZ Protein Complex Reveals Negative Feedback Regulation of Pantothenate Biosynthesis by Coenzyme A

2015 ◽  
Vol 22 (4) ◽  
pp. 492-503 ◽  
Author(s):  
Diana C.F. Monteiro ◽  
Vijay Patel ◽  
Christopher P. Bartlett ◽  
Shingo Nozaki ◽  
Thomas D. Grant ◽  
...  
Keyword(s):  
Negative Feedback ◽  
Protein Complex ◽  
Coenzyme A ◽  
Feedback Regulation ◽  
Negative Feedback Regulation ◽  
Pantothenate Biosynthesis

scholarly journals Negative-feedback regulation of ATP release: ATP release from cardiomyocytes is strictly regulated during ischemia

2011 ◽  
Vol 416 (3-4) ◽  
pp. 409-415 ◽  
Author(s):  
Satohiko Kunugi ◽  
Sadahiro Iwabuchi ◽  
Daisuke Matsuyama ◽  
Takaharu Okajima ◽  
Koichi Kawahara
Keyword(s):  
Negative Feedback ◽  
Atp Release ◽  
Feedback Regulation ◽  
Negative Feedback Regulation
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