The acute phase response increases the ability of urea synthesis but decreases hepatic gene expression of the rate-limiting urea cycle enzyme

2002 ◽  
Vol 36 ◽  
pp. 257
Author(s):  
Susanne Schouw Nielsen ◽  
Thorbjarn Grofte ◽  
Niels Tygstrup ◽  
Hendrik Vilstrup
Keyword(s):  
Gene Expression ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Urea Cycle ◽  
Phase Response ◽  
Hepatic Gene Expression ◽  
Urea Synthesis ◽  
Cycle Enzyme ◽  
Rate Limiting ◽  
Urea Cycle Enzyme

Regulation of urea synthesis during the acute-phase response in rats

2013 ◽  
Vol 304 (7) ◽  
pp. G680-G686 ◽  
Author(s):  
Karen Louise Thomsen ◽  
Niels Jessen ◽  
Andreas Buch Møller ◽  
Niels Kristian Aagaard ◽  
Henning Grønbæk ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Urea Cycle ◽  
Acute Phase Proteins ◽  
Phase Response ◽  
Mrna Levels ◽  
Urea Synthesis ◽  
Cycle Enzyme ◽  
Tnf Α ◽  
Urea Cycle Enzyme

The acute-phase response is a catabolic event involving increased waste of amino-nitrogen (N) via hepatic urea synthesis, despite an increased need for amino-N incorporation into acute-phase proteins. This study aimed to clarify the regulation of N elimination via urea during different phases of the tumor necrosis factor-α (TNF-α)-induced acute-phase response in rats. We used four methods to study the regulation of urea synthesis: We examined urea cycle enzyme mRNA levels in liver tissue, the hepatocyte urea cycle enzyme proteins, the in vivo capacity of urea-N synthesis (CUNS), and known humoral regulators of CUNS at 1, 3, 24, and 72 h after TNF-α injection (25 μg/kg iv rrTNF-α) in rats. Serum acute-phase proteins and their liver mRNA levels were also measured. The urea cycle enzyme mRNA levels acutely decreased and then gradually normalized, whereas the urea cycle enzyme proteins remained essentially unchanged over time. The CUNS rose after 3 h and then normalized. The acute-phase response was fully activated at 24 h with markedly increased serum levels of the acute-phase proteins. TNF-α acutely upregulated the CUNS. Later, despite the fully established 24-h acute-phase response and the decreased activity of the urea cycle enzyme genes, there was no change in the urea cycle enzyme proteins or the CUNS. Thus in no phase after the initiation of the acute-phase response was in vivo urea synthesis orchestrated in combination with acute-phase protein synthesis so as to limit N waste.

Assessment of the ovine acute phase response and hepatic gene expression in response to Escherichia coli endotoxin

2006 ◽  
Vol 113 (1-2) ◽  
pp. 113-124 ◽  
Author(s):  
Leah C. Kabaroff ◽  
Alexander Rodriguez ◽  
Margaret Quinton ◽  
Herman Boermans ◽  
Niel A. Karrow
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Hepatic Gene Expression

scholarly journals STAT3/NFκB Interplay in the Regulation of α2-Macroglobulin Gene Expression During Rat Liver Development and the Acute Phase Response

IUBMB Life ◽  
2007 ◽  
Vol 59 (3) ◽  
pp. 170-178 ◽  
Author(s):  
Aleksandra Uskokovic ◽  
Svetlana Dinic ◽  
Mirjana Mihailovic ◽  
Ilijana Grigorov ◽  
Svetlana Ivanovic-Matic ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Liver Development ◽  
Α2 Macroglobulin

Unchanged capacity of urea synthesis during acute phase response in rats

2010 ◽  
Vol 41 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Karen L. Thomsen ◽  
Niels K. Aagaard ◽  
Susanne S. Nielsen ◽  
Henning Grønbaek ◽  
Niels Jessen ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Urea Synthesis

scholarly journals Ah receptor represses acute-phase response gene expression without binding to its cognate response element

2009 ◽  
Vol 89 (6) ◽  
pp. 695-707 ◽  
Author(s):  
Rushang D Patel ◽  
Iain A Murray ◽  
Colin A Flaveny ◽  
Ann Kusnadi ◽  
Gary H Perdew
Keyword(s):  
Gene Expression ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Ah Receptor ◽  
Response Gene ◽  
Response Element
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