Fibrinogen inclusions in liver cells: A new type of ground-glass hepatocyte. Incidence and significance in liver biopsies

The aim – to study some histopathological features of the iron overload syndromeformation in patients suffering from steatohepatitis depending on its etiology.Material and methods. Liver biopsies were analyzed in 30 patients with nonalcoholicsteatohepatitis (NASH) and 20 patients with alcoholic steatohepatitis (ASH). Themorphological examination of the liver was carried out according to the standardmethods. The histopathological features of the liver were established on the basis ofthe method of staining with hematoxylin and eosin, with the confirmation, in case ofnecessity, by the histochemical methods - staining for fat (the method with Sudan-III) andcollagen fibers (the method with chromotropic-water blue according to N.Z Slinchenko).Microspectrophotometric studies were conducted using a cytological analyzer withsoftware "VideoTest - Size 5.0" (2000).Results. The same regularities for both trivalent iron and twovalent iron were revealed asa whole in the liver cells at histological investigations, that is in ASH the optical densityis at the average 1.3 (p<0.05) times higher than in NASH. The staining on trivalent ironis less intensive, than on the twovalent iron. There is probable iron overload syndrome inthe alcoholic steatohepatitis patients with increased uptake of it both 2+- and 3+- valentforms in the liver cells – reticuloendotheliocytes and hepatocytesConclusions. Iron overload syndrome with increased uptake of it for both 2+- and3+-valent takes place in the liver cells – endotheliocytes and hepatocytes – in patientswith alcoholic steatohepatitis. At alcoholic steatohepatitis the optical density withhistochemical staining on iron 2+ in reticuloendotheliocytes of the liver is 1.2 times andin hepatocytes – 1.3 times higher than at non-alcoholic steatohepatitis irrespective ofthe presence or absence of anemia. At alcoholic steatohepatitis the optical density ofhistochemical iron staining 3+ in reticuloendotheliocytes of the liver and hepatocytesis 1.3 times higher than in non-alcoholic steatohepatitis, and is irrespective of anemiapresence as well.

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Detection of HBsAg containing cells in liver biopsies by different stains and classification of positively reacting ground-glass hepatocytes

Virchows Archiv A Pathological Anatomy and Histology ◽

10.1007/bf00428227 ◽

1979 ◽

Vol 384 (3) ◽

pp. 245-261 ◽

Cited By ~ 12

Author(s):

F. Borchard ◽

V. Gussmann

Keyword(s):

Ground Glass ◽

Liver Biopsies

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Different Types of Ground Glass Hepatocytes in Human Liver Biopsies: Morphology, Occurrence and Diagnostic Significance

Scandinavian Journal of Gastroenterology ◽

10.1080/00365521.1976.12097081 ◽

1976 ◽

Vol 11 (2) ◽

pp. 113-119

Author(s):

Per Thomsen ◽

Hemming Poulsen ◽

Palle Petersen

Keyword(s):

Human Liver ◽

Ground Glass ◽

Diagnostic Significance ◽

Human Liver Biopsies ◽

Different Types ◽

Liver Biopsies

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Transient Ground Glass Hepatocellular Change following a Period of Glue Sniffing in a Patient with Underlying Benign Recurrent Cholestatic Jaundice

Medicine Science and the Law ◽

10.1177/002580248402400207 ◽

1984 ◽

Vol 24 (2) ◽

pp. 102-106 ◽

Cited By ~ 2

Author(s):

B. Conroy ◽

C. R. Pennington

Keyword(s):

Liver Biopsy ◽

Temporal Relationship ◽

Morphological Features ◽

Cholestatic Jaundice ◽

Ground Glass ◽

Adolescent Male ◽

Supportive Evidence ◽

Obese Adolescent ◽

Liver Biopsies ◽

Rare Opportunity

This report describes the liver biopsy findings in an obese adolescent male suffering from benign recurrent intrahepatic cholestatic jaundice who developed transient focal feathery swelling and ground glass hepatocellular change within several weeks of indulging in glue sniffing. Clinically the situation provided a rare opportunity to observe the nature and progress of morphological features by means of sequential liver biopsies taken for the investigation of the patient's underlying jaundice. The temporal relationship between the confessed period of glue sniffing and appearance of the hepatocellular abnormality provides, in our opinion, strong supportive evidence of a definite link between the two.

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Crystalloid Inclusions in Hepatocyte Mitochondria and Their Similarity to Cytoplasmic Crystalloids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051001 ◽

1974 ◽

Vol 32 ◽

pp. 198-199

Author(s):

Odell T. Minick ◽

Hidejiro Yokoo

Keyword(s):

Liver Disease ◽

Alcoholic Liver Disease ◽

Special Interest ◽

Structural Variations ◽

Mitochondrial Alterations ◽

The Matrix ◽

Crystalloid Inclusions ◽

Liver Biopsies

Mitochondrial alterations were studied in 25 liver biopsies from patients with alcoholic liver disease. Of special interest were the morphologic resemblance of certain fine structural variations in mitochondria and crystalloid inclusions. Four types of alterations within mitochondria were found that seemed to relate to cytoplasmic crystalloids.Type 1 alteration consisted of localized groups of cristae, usually oriented in the long direction of the organelle (Fig. 1A). In this plane they appeared serrated at the periphery with blind endings in the matrix. Other sections revealed a system of equally-spaced diagonal lines lengthwise in the mitochondrion with cristae protruding from both ends (Fig. 1B). Profiles of this inclusion were not unlike tangential cuts of a crystalloid structure frequently seen in enlarged mitochondria described below.

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The Microstructure of Shock-Synthesized Diamond

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006163x ◽

1967 ◽

Vol 25 ◽

pp. 324-325

Author(s):

Lucien F. Trueb

Keyword(s):

Single Crystals ◽

Preferred Orientation ◽

High Magnification ◽

Texture Pattern ◽

Shock Process ◽

New Type ◽

Diffraction Diagram

A new type of synthetic industrial diamond formed by an explosive shock process has been recently developed by the Du Pont Company. This material consists of a mixture of two basically different forms, as shown in Figure 1: relatively flat and compact aggregates of acicular crystallites, and single crystals in the form of irregular polyhedra with straight edges.Figure 2 is a high magnification micrograph typical for the fibrous aggregates; it shows that they are composed of bundles of crystallites 0.05-0.3 μ long and 0.02 μ. wide. The selected area diffraction diagram (insert in Figure 2) consists of a weak polycrystalline ring pattern and a strong texture pattern with arc reflections. The latter results from crystals having preferred orientation, which shows that in a given particle most fibrils have a similar orientation.

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