PATHOHISTOLOGICAL PECULIARITIES OF THE FORMATION OF IRON OVERLOAD SYNDROME IN PATIENTS WITH STEATOHEPATITIS DEPENDING ON ITS ETIOLOGY

The aim – to study some histopathological features of the iron overload syndromeformation in patients suffering from steatohepatitis depending on its etiology.Material and methods. Liver biopsies were analyzed in 30 patients with nonalcoholicsteatohepatitis (NASH) and 20 patients with alcoholic steatohepatitis (ASH). Themorphological examination of the liver was carried out according to the standardmethods. The histopathological features of the liver were established on the basis ofthe method of staining with hematoxylin and eosin, with the confirmation, in case ofnecessity, by the histochemical methods - staining for fat (the method with Sudan-III) andcollagen fibers (the method with chromotropic-water blue according to N.Z Slinchenko).Microspectrophotometric studies were conducted using a cytological analyzer withsoftware "VideoTest - Size 5.0" (2000).Results. The same regularities for both trivalent iron and twovalent iron were revealed asa whole in the liver cells at histological investigations, that is in ASH the optical densityis at the average 1.3 (p<0.05) times higher than in NASH. The staining on trivalent ironis less intensive, than on the twovalent iron. There is probable iron overload syndrome inthe alcoholic steatohepatitis patients with increased uptake of it both 2+- and 3+- valentforms in the liver cells – reticuloendotheliocytes and hepatocytesConclusions. Iron overload syndrome with increased uptake of it for both 2+- and3+-valent takes place in the liver cells – endotheliocytes and hepatocytes – in patientswith alcoholic steatohepatitis. At alcoholic steatohepatitis the optical density withhistochemical staining on iron 2+ in reticuloendotheliocytes of the liver is 1.2 times andin hepatocytes – 1.3 times higher than at non-alcoholic steatohepatitis irrespective ofthe presence or absence of anemia. At alcoholic steatohepatitis the optical density ofhistochemical iron staining 3+ in reticuloendotheliocytes of the liver and hepatocytesis 1.3 times higher than in non-alcoholic steatohepatitis, and is irrespective of anemiapresence as well.

Productive preparation of N-doped carbon dots from sodium lignosulfonate/melamine formaldehyde foam and its fluorescence detection of trivalent iron ions

Taking advantage of the rich carbon source of waste foam, N-doped carbon dots were prepared by calcining sodium lignosulfonate/melamine formaldehyde foam in one step, which shows preferential fluorescence detection for iron ions.

Sustainable Oxygen Evolution Catalysis - Electrochemical Generation of Mössbauerite via Corrosion Engineering of Steel

A versatile electrochemical corrosion process was used to generate green rust (GR) on a steel substrate and to transform it into the trivalent iron-only oxygen evolution catalyst mössbauerite (GR*) upon...

Rusty-colored representatives of genus Circinaria Link. (lichenized ascomycetes) in arid habitats of Bogdinsko-Baskunchakskiy nature reserve

Diversity of Aspicilia desertorum s. l. was studied in Bogdinsko-Baskunchakskiy reserve (Astrakhanregion, Russia). The group comprises eight species in the region. Six of them include specimens with rusty-colored thalli.We found that trivalent iron in the cortex of the studied species is not a taxonomic character but has a connection withchemistry of the substrate and may act as a sun-screen, decreasing levels of UV-B reaching the photobiont.