Endothelin-1 in the rat bile duct ligation model of hepatopulmonary syndrome: correlation with pulmonary dysfunction

Hepatopulmonary syndrome (HPS) causes impaired oxygenation due to intrapulmonary vasodilatation in patients with cirrhosis. Chronic common bile duct ligation (CBDL) in the rat results in gas-exchange abnormalities similar to HPS, but intrapulmonary vasodilatation has not been evaluated. We assess intrapulmonary vasodilatation, measured in vivo, after CBDL. Sham, 2- and 5-wk CBDL, and 3-wk partial portal vein ligated (PVL) rats had hepatic and lung injury, portal pressure, and arterial blood gases assessed. The pulmonary microcirculation was evaluated by injecting microspheres (size range 5.5-10 microm) intravenously and measuring the size and number of microspheres bypassing the lungs in arterial blood. CBDL animals developed progressive hepatic injury and portal hypertension accompanied by gas-exchange abnormalities and intrapulmonary vasodilatation. PVL animals, with a similar degree of portal hypertension, did not develop intrapulmonary vasodilatation or abnormal gas exchange. No lung injury was observed. CBDL, but not PVL, causes progressive intrapulmonary vasodilatation, which accompanies worsening arterial gas exchange. These findings validate CBDL as a model to study HPS.

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Effects of melatonin on liver and lung tissues of animals with bile duct ligation-induced hepatopulmonary syndrome

Journal of Pulmonology and Respiratory Research ◽

10.29328/journal.jprr.1001033 ◽

2021 ◽

Vol 5 (1) ◽

pp. 097-105

Author(s):

Dal Bosco Adriane ◽

Colares Josieli Raskopf ◽

Bona Sílvia ◽

De Andrade Lívia Barboza ◽

Forgiarini Jr. Luiz Alberto ◽

...

Keyword(s):

Bile Duct ◽

Wistar Rats ◽

Histological Analysis ◽

Bile Duct Ligation ◽

Hepatopulmonary Syndrome ◽

Lung Parenchyma ◽

Antioxidant Effect ◽

Biliary Cirrhosis ◽

Duct Ligation ◽

Male Wistar Rats

The objective was to assess the antioxidant effect of melatonin (MLT) on liver and lung tissues of animals with bile duct ligation (BDL)-induced hepato-pulmonary syndrome (HPS). A model of BDL-induced biliary cirrhosis was used in male Wistar rats. Results suggest that MLT has an antioxidant effect on liver and lung tissues in animals with BDL-induced HPS by higher activity of antioxidant enzymes in the group HPS treated with MLT and the histological analysis of lung parenchyma showing decreased damage in this same group, including other analysis described below.

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Serum Alkaline Phosphodiesterase I in Experimental Biliary Obstruction in the Rat

Clinical Science ◽

10.1042/cs0670647 ◽

1984 ◽

Vol 67 (6) ◽

pp. 647-652 ◽

Cited By ~ 1

Author(s):

Stuart R. Simpson ◽

K. Rahman ◽

D. Billington

Keyword(s):

Alkaline Phosphatase ◽

Bile Duct ◽

Biliary Obstruction ◽

Bile Duct Ligation ◽

Serum Alkaline Phosphatase ◽

Gel Filtration ◽

Rat Serum ◽

Duct Ligation ◽

Rat Bile ◽

Molecular Weight Form

1. Alkaline phosphodiesterase I was present in rat liver at approx. 100-fold greater activity than alkaline phosphatase, and in rat bile at approx. 25-fold greater activity. 2. Rat serum alkaline phosphodiesterase I was increased 6-fold whilst serum alkaline phosphatase was increased only 2-fold 96 h after bile duct ligation. 3. In contrast to alkaline phosphatase, hepatic alkaline phosphodiesterase I was not affected by bile duct ligation, suggesting its raised serum activity was due to bile regurgitation rather than overspill of the enzyme from liver into blood. 4. Gel filtration showed that 8 and 96 h after bile duct ligation the serum contained a high molecular weight form of alkaline phosphodiesterase I. 5. It is suggested that alkaline phosphodiesterase I offers a potentially useful indicator of biliary obstruction in the rat.

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