scholarly journals Effects of melatonin on liver and lung tissues of animals with bile duct ligation-induced hepatopulmonary syndrome

2021 ◽  
Vol 5 (1) ◽  
pp. 097-105
Author(s):  
Dal Bosco Adriane ◽  
Colares Josieli Raskopf ◽  
Bona Sílvia ◽  
De Andrade Lívia Barboza ◽  
Forgiarini Jr. Luiz Alberto ◽  
...  
Keyword(s):  
Bile Duct ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Bile Duct Ligation ◽  
Hepatopulmonary Syndrome ◽  
Lung Parenchyma ◽  
Antioxidant Effect ◽  
Biliary Cirrhosis ◽  
Duct Ligation ◽  
Male Wistar Rats

The objective was to assess the antioxidant effect of melatonin (MLT) on liver and lung tissues of animals with bile duct ligation (BDL)-induced hepato-pulmonary syndrome (HPS). A model of BDL-induced biliary cirrhosis was used in male Wistar rats. Results suggest that MLT has an antioxidant effect on liver and lung tissues in animals with BDL-induced HPS by higher activity of antioxidant enzymes in the group HPS treated with MLT and the histological analysis of lung parenchyma showing decreased damage in this same group, including other analysis described below.

Magnesium protects against bile duct ligation-induced liver injury in male Wistar rats

2015 ◽  
Vol 28 (1) ◽  
pp. 32-45
Author(s):  
Tahereh Eshraghi ◽  
Akram Eidi ◽  
Pejman Mortazavi ◽  
Ahmad Asghari ◽  
Seyyed Mohammad Tavangar
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Wistar Rats ◽  
Bile Duct Ligation ◽  
Duct Ligation ◽  
Male Wistar Rats

scholarly journals The effects of bile duct ligation on motor cortex region morphology and aquaporin 4 protein concentration in male Wistar rats

2019 ◽  
Vol 6 (1) ◽  
pp. 1-9
Author(s):  
Delaram Eslimi Esfahani ◽  
◽  
Shahrbanoo Oryan ◽  
Mohammad Nabiuni ◽  
Talieh Sadat Hosseinynia ◽  
...  
Keyword(s):  
Bile Duct ◽  
Motor Cortex ◽  
Protein Concentration ◽  
Wistar Rats ◽  
Bile Duct Ligation ◽  
Aquaporin 4 ◽  
Duct Ligation ◽  
Male Wistar Rats

scholarly journals Study of the relation between biliary cirrhosis and hepatopulmonary syndrome and its reversibility among young rats submitted to common bile duct ligation

2018 ◽  
Vol 97 (3) ◽  
pp. 376-377
Author(s):  
Leonardo Ervolino Corbi ◽  
Maria Julia De Aro Braz ◽  
Ana Cristina Aoun Tannuri ◽  
Maria Cecília De Mendonça Coelho ◽  
Suellen Serafini ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Duct Ligation ◽  
Hepatopulmonary Syndrome ◽  
Biliary Cirrhosis ◽  
Common Bile Duct Ligation ◽  
Young Rats ◽  
Duct Ligation

A new method for the experimental induction of secundary biliary cirrhosis in wistar rats

2001 ◽  
Vol 16 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Gracinda De Lourdes Jorge ◽  
Luiz Sergio Leonardi ◽  
Ilka de Fatima Santana Ferreira Boin ◽  
Orlando de Castro e Silva Jr ◽  
Cecilia Amelia Fazzio Escanhoela
Keyword(s):  
Bile Duct ◽  
Morphological Study ◽  
Wistar Rats ◽  
Hepatic Duct ◽  
Control Group ◽  
Sham Operation ◽  
Biliary Cirrhosis ◽  
Technical Failure ◽  
Duct Obstruction ◽  
Duct Ligation

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.

scholarly journals Liver histological, portal flow and plasmatic nitric oxide alterations caused by biliary obstruction and drainage in rats

2008 ◽  
Vol 23 (suppl 1) ◽  
pp. 2-7 ◽  
Author(s):  
Miguel Angel Dias ◽  
Reginaldo Ceneviva ◽  
Jorge Elias Jr. ◽  
Sergio Zucoloto ◽  
Caroline Floreoto Baldo ◽  
...  
Keyword(s):  
Bile Duct ◽  
Biliary Obstruction ◽  
Bile Duct Ligation ◽  
Sham Operation ◽  
Portal Flow ◽  
Histological Alterations ◽  
Duct Occlusion ◽  
Duct Ligation ◽  
Male Wistar Rats ◽  
Periodic Evaluation

PURPOSE: To evaluate liver alterations caused by biliary obstruction and drainage. METHODS: Thirty-nine male Wistar rats were randomly distributed in 4 groups: BO (n=18) bile duct ligation for 20 days, with a periodic evaluation of liver histological alterations, Doppler echography portal flow and measurements of NO and malondialdehyde (MDA); BO/DB (n=13) bile duct occlusion for 20 days followed by biliary drainage by choledochoduodenal anastomosis, 5 days follow-up, same BO group parameters evaluations; group CED (n=4) sham operation and portal flow evaluation trough 20 days; CHB (n=4) sham operation, with hepatic biopsy on 25th day and followed-up trough 25 days, by the same parameters of group BO, with exception of portal flow. Direct bilirubin (DB) and alkaline phosphatase (AP) were evaluated in the group BO, BO/DB and CHB. RESULTS: The bile duct ligation led to an increase of DB and AP, development of liver histological alterations, reduction of portal flow and increase of plasmatic NO and of MDA levels. The bile duct clearing resulted in a reduction of DB, AP, NO, MDA histological alterations and increase of portal flow. CONCLUSION: The biliary occlusion resulted in cholestasis and portal flow reduction, besides the increase of plasmatic NO and of hepatic MDA levels, and histological liver alterations, with a tendency of normalization after the bile duct clearing.

scholarly journals Serum and Hepatic Autofluorescence as a Real-Time Diagnostic Tool for Early Cholestasis Assessment

2018 ◽  
Vol 19 (9) ◽  
pp. 2634 ◽  
Author(s):  
Anna Croce ◽  
Giovanni Bottiroli ◽  
Laura Di Pasqua ◽  
Clarissa Berardo ◽  
Veronica Siciliano ◽  
...  
Keyword(s):  
Bile Duct ◽  
Real Time ◽  
Diagnostic Tool ◽  
Bile Duct Ligation ◽  
Fiber Optic Probe ◽  
Duct Ligation ◽  
Male Wistar Rats ◽  
Optic Probe ◽  
Oxidative Products ◽  
Liver Changes

While it is well established that various factors can impair the production and flow of bile and lead to cholestatic disease in hepatic and extrahepatic sites, an enhanced assessment of the biomarkers of the underlying pathophysiological mechanisms is still needed to improve early diagnosis and therapeutic strategies. Hence, we investigated fluorescing endogenous biomolecules as possible intrinsic biomarkers of molecular and cellular changes in cholestasis. Spectroscopic autofluorescence (AF) analysis was performed using a fiber optic probe (366 nm excitation), under living conditions and in serum, on the livers of male Wistar rats submitted to bile duct ligation (BDL, 24, 48, and 72 h). Biomarkers of liver injury were assayed biochemically. In the serum, AF analysis distinctly detected increased bilirubin at 24 h BDL. A continuous, significant increase in red-fluorescing porphyrin derivatives indicated the subversion of heme metabolism, consistent with an almost twofold increase in the serum iron at 72 h BDL. In the liver, changes in the AF of NAD(P)H and flavins, as well as lipopigments, indicated the impairment of mitochondrial functionality, oxidative stress, and the accumulation of oxidative products. A serum/hepatic AF profile can be thus proposed as a supportive diagnostic tool for the in situ, real-time study of bio-metabolic alterations in bile duct ligation (BDL) in experimental hepatology, with the potential to eventually translate to clinical diagnosis.

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