Analysis of habituated embryogenic lines in Asparagus officinalis L.: growth characteristics, hormone content and ploidy level of calli and regenerated plants

Plant Science ◽  
2000 ◽  
Vol 160 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Anne Limanton-Grevet ◽  
Bruno Sotta ◽  
Spencer Brown ◽  
Marc Jullien
Keyword(s):  
Ploidy Level ◽  
Growth Characteristics ◽  
Asparagus Officinalis ◽  
Hormone Content ◽  
Regenerated Plants ◽  
Embryogenic Lines

Optimization of biotechnological process clonal micropropagation in vitro of Asparagus officinalis L.

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Y Kolomiiets ◽  
◽  
A Skuba ◽  
Keyword(s):  
Culture Medium ◽  
Asparagus Officinalis ◽  
Skoog Medium ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Macro And Micronutrients ◽  
Indole 3 Acetic Acid

The study presents the results of obtaining regenerated plants of asparagus from seeds. Surface sterilizing the seeds by 0,75% sodium hypochlorite for 30 min is effective, during this obtained 83% viable sterile plants. The Murashige and Skoog medium supplemented with 6‑benzylaminopurine (2 mg/L), inositol (100 mg/L) and thiamine (0,4 mg/L) was found to be the best for seed germination. The expediency of using kinetin (1 mg/L) as a growth regulator to obtain a homogeneous plant material was established. The reproduction coefficient was 6,0. Only 11% of the explants formed callus. For the selection needs and production of somaclonal variants, the use of the culture medium with indole-3-acetic acid (0,2 mg/L) and 6‑benzylaminopurine (1 mg/L) is justified. In this condition reproduction coefficient was 3,7, and the level of different intensity callusogenesis was 59%. The rooting of obtained plants was performed in Murashige and Skoog medium supplemented with a half dose of macro- and micronutrients and growth regulators. Rooting frequency was up to 63%. The knowledge of hormonal requirements helps to promote isolated tissue and cells technologies of asparagus with purpose of rapid propagation and obtaining healthy, high-quality planting material.

scholarly journals Chromosome Number Variation of Callus Cells and Regenerated Plants in Asparagus officinalis L.

1992 ◽  
Vol 9 (3) ◽  
pp. 169-175 ◽  
Author(s):  
Hajime ARAKI ◽  
Hiroko SHIMAZAKI ◽  
Yukimasa HIRATA ◽  
Toshiro ORIDATE ◽  
Takashi HARADA ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Asparagus Officinalis ◽  
Regenerated Plants ◽  
Number Variation ◽  
Chromosome Number Variation

Isolation and Characterization of Long-term Embryogenic Lines in Asparagus officinalis L.

1994 ◽  
Vol 144 (2) ◽  
pp. 194-200 ◽  
Author(s):  
B. Delbreil ◽  
I. Goebel-Tourand ◽  
C. Lefrançois ◽  
M. Jullien
Keyword(s):  
Asparagus Officinalis ◽  
Isolation And Characterization ◽  
Embryogenic Lines

scholarly journals Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.)

1998 ◽  
Vol 21 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Leonardo Galli ◽  
Judith Viégas ◽  
Eliane Augustin ◽  
Marcia Ines Eckert ◽  
João Baptista da Silva
Keyword(s):  
Anther Culture ◽  
Genomic Instability ◽  
Chromosomal Abnormalities ◽  
Asparagus Officinalis ◽  
Pollen Mother Cells ◽  
Regenerated Plants ◽  
Mother Cells

Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed.

scholarly journals Identification of Ploidy Level of the Regenerated Plants by Anther Culture in Rice.

1994 ◽  
Vol 44 (1) ◽  
pp. 19-22
Author(s):  
Kazuhiro Nakamura ◽  
Hiroaki Suzuki ◽  
Kazumi Hattori ◽  
Yuzo Futsuhara
Keyword(s):  
Anther Culture ◽  
Ploidy Level ◽  
Regenerated Plants
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