Optimization of biotechnological process clonal micropropagation in vitro of Asparagus officinalis L.

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Y Kolomiiets ◽  
◽  
A Skuba ◽  
Keyword(s):  
Culture Medium ◽  
Asparagus Officinalis ◽  
Skoog Medium ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Macro And Micronutrients ◽  
Indole 3 Acetic Acid

The study presents the results of obtaining regenerated plants of asparagus from seeds. Surface sterilizing the seeds by 0,75% sodium hypochlorite for 30 min is effective, during this obtained 83% viable sterile plants. The Murashige and Skoog medium supplemented with 6‑benzylaminopurine (2 mg/L), inositol (100 mg/L) and thiamine (0,4 mg/L) was found to be the best for seed germination. The expediency of using kinetin (1 mg/L) as a growth regulator to obtain a homogeneous plant material was established. The reproduction coefficient was 6,0. Only 11% of the explants formed callus. For the selection needs and production of somaclonal variants, the use of the culture medium with indole-3-acetic acid (0,2 mg/L) and 6‑benzylaminopurine (1 mg/L) is justified. In this condition reproduction coefficient was 3,7, and the level of different intensity callusogenesis was 59%. The rooting of obtained plants was performed in Murashige and Skoog medium supplemented with a half dose of macro- and micronutrients and growth regulators. Rooting frequency was up to 63%. The knowledge of hormonal requirements helps to promote isolated tissue and cells technologies of asparagus with purpose of rapid propagation and obtaining healthy, high-quality planting material.

scholarly journals OBTAINING HIGH-QUALITY PLANTING MATERIAL OF FOREST BERRY PLANTS BY CLONAL MICROPROPAGATION FOR RESTORATION OF CUTOVER PEATLANDS

2021 ◽  
pp. 21-29
Author(s):  
Sergey Makarov ◽  
◽  
Irina Kuznetsova ◽  
Anton Chudetsky ◽  
Sergey Rodin ◽  
...  
Keyword(s):  
High Efficiency ◽  
Sharp Decrease ◽  
Environmental Damage ◽  
Agent Based ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Mercuric Chloride Solution ◽  
Regenerated Plants ◽  
Berry Crops

The results of studies on the improvement of technology for producing highquality planting material of half-high blueberry and Arctic bramble by the method of clonal micropropagation are presented in the current paper. Creation of forest berry plantations in peat extraction areas allows reducing environmental damage and significantly increasing the efficiency of the timber industry. In recent decades, there has been an increasing interest in the creation of forest berry plantations on drained and cutover peatlands in Russia and other countries. It is necessary to use varietal planting material for the successful cultivation of forest berry plants on an industrial scale. Clonal micropropagation is the most effective of the vegetative methods for obtaining planting material, which allows receiving a huge amount of healthy planting material all year round in the conditions of a small laboratory area. Chloride-free ecosterilizer and bleaching agent based on sodium hypochlorite “Belizna” with an exposure of 15 and 20 min showed high efficiency in sterilization of explants of half-high blueberry and Arctic bramble. The highest viability of explants of the studied forest berry crops was observed when sterilized with a 0.1 % mercuric chloride solution and 15 min exposure, and its sharp decrease at 20 min exposure. At the stage of micropropagation, with an increase in the concentration of cytokinin 6-BAP from 0.5 to 1.0 mg/L on the nutrient Woody Plant Medium the number of shoots in regenerated plants of half-high blueberry (Northcountry and Northblue cultivars) and Arctic bramble (Anna and Sofia cultivars) increased. The effect of the concentration of IBA-derived auxin on the number and length of roots of regenerated plants was observed at the in vitro rooting stage.

scholarly journals Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro

2022 ◽  
Vol 51 (4) ◽  
pp. 712-722
Author(s):  
Elena Kulikova ◽  
Sergey Makarov ◽  
Irina Kuznetsova ◽  
Anton Chudetsky
Keyword(s):  
Culture Medium ◽  
Root Formation ◽  
Plant Root ◽  
Total Length ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Number Of Leaves ◽  
Effective Growth

Introduction. The demand for honeysuckle berries and planting material is growing. Clonal micropropagation is the most effective method for industrial plantations. The research objective was to study the effect of cytokinins and auxins on Russian and Canadian honeysuckle microshoots and roots. Study objects and methods. The study featured regenerated honeysuckle (Lonicera edulis Turcz.) of three Russian cultivars (Bakcharsky Velikan, Doch Velikana, Yugana) and two Canadian cultivars (Boreal Beauty, Boreal Beast). The experiment focused on the effect of sterilizing agents and sterilization time on the viability of honeysuckle explants at the stage of culture introduction in vitro. The effect of the growth regulator Cytodef in the QL nutrient medium on organogenesis was studied at the stage of micropropagation proper, the effect of auxin IBA on plant root formation – at the stage of rooting in vitro. Results and discussion. The greatest viability of honeysuckle explants (80–94%) was registered in the samples affected by Lizoformin 3000 (5%) and silver nitrate (0.2%) as sterilizing agents with a sterilization time of 10 min at the stage of in vitro culture introduction. The biggest quantity (8.8 pcs.) and total length (40.1 cm) of microshoots were observed when the content of cytokinin Cytodef in the culture medium QL was 0.3 mg/L at the stage micropropagation proper. The Boreal Beast cultivar had the largest total length of shoots (29.0 cm). The biggest quantity (5.5 pcs.) and total length (30.8 cm) of roots resulted from 0.5 mg/L of auxin IBA at the stage of rooting in vitro. Coconut substrate produced the highest survival rate (92–99%) at the stage of adaptation to non-sterile conditions in vivo, with the greatest number of leaves (8.1–10.2 pcs.) observed in Canadian cultivars. Conclusion. Cytodef and IBA proved to be effective growth-regulating substances for microplants of Russian and Canadian honeysuckle cultivars in vitro, which makes them promising for berry plantations.

scholarly journals Morphogenesis of Miscanthus × Giganteus in vitro

2018 ◽  
Vol 5 (2) ◽  
pp. 13-17
Author(s):  
O. Klyachenko ◽  
O. Nekrut
Keyword(s):  
Statistical Processing ◽  
Meristem Culture ◽  
Culture Methods ◽  
Skoog Medium ◽  
Plant Cultivation ◽  
Planting Material ◽  
Miscanthus Giganteus ◽  
Murashige And Skoog Medium

Aim. To obtain planting material of Miscanthus × giganteus in a large amount in order to study the peculiari- ties of morphogenesis of isolated meristem culture. Methods. The explants were sterilized using 70 % etha- nol and 0.1 % mercury bichloride, after that, the methods of plant cultivation and reproduction in vitro and statistical processing of the obtained data was performed. Results. The study presents the results of obtaining the regenerate plants from dormant buds of 3-year-old rhizomes of Miscanthus × giganteus. The Murashige and Skoog medium supplemented with 6-benzylaminopurine (BAP) (0.75 mg/L) and kinetin (1.2 mg/L) was found to be the best for regeneration of microsprouts, providing the maximum value of the regeneration index (95 %) and contributing to the development of the main sprouts and tillering. The obtained sprouts were rooted in Murashige and Skoog medium supplemented with half-dose of macro- and micronutrients without growth regulators. In doing so, rooting index made up 95.0–97.0 %. The best substrate for the adaptation of miscan- thus plants in vivo was found to be a mixture of peat, sand, and perlite in a ratio of 2 : 1 : 1, respectively. In this substrate, plant establishment reached 91 %. Conclusions. The peculiarities of morphogenesis in Miscan- thus × giganteus isolated meristem were studied. At the same time, the index of sprouts regeneration varied from 90.0 to 100.0 %. In addition, the development of the main sprout and multiple tillering occurred in 85.0– 100.0 % explant. The highest survivability of micro-plants (91–95 %) was provided in the peat-sand-perlite substrate in a ratio of 2 : 1 : 1.

scholarly journals Application efficiency of Superstim modifications in low doses on Lonicera caerulea adaptation stage and it’s after-effect during subsequent growing

2019 ◽  
pp. 53-61
Author(s):  
S. V. Akimova ◽  
N. A. Semenova ◽  
N. N. Malevannaya ◽  
A. N. Vikulina ◽  
V. V. Kirkach ◽  
...  
Keyword(s):  
Survival Rates ◽  
Wide Distribution ◽  
Low Doses ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Regenerated Plants ◽  
After Effect ◽  
Short Time ◽  
New Generation

Relevance. In recent years, interest in the edible honeysuckle culture has increased in Russia, the wide distribution of which is hampered by the lack of quality planting material. The technology of clonal micropropagation allows for a short time to obtain a large amount of honeysuckle planting material, more than a thousand regenerated plants per year from one meristematic apex introduced into an in vitro culture. It is hundreds of times more than in traditional methods of vegetative propagation. Adaptation to non-sterile conditions is the final and most crucial stage of clonal micropropagation, the loss of which can be from 50 to 90%. It should be noted that there is practically no research on how the further development of adapted honeysuckle plants takes place during subsequent growing.Methods. Researching of growth regulators of the new generation Superstim 1 and Superstim 2 effect in low and ultra-low doses on the survival rates and development of honeysuckle plants at the stages of adaptation subsequent growing.Results. Superstim 1 is more effective at physiological concentrations – 1 x 10-7 and in the field of ultra-low doses – 1 x 10-14, 1 x 10-15%. At the stage of subsequent growing, a positive after-effect of physiological concentrations – 1x10-3 and 1x10-7 was observed, and an ultra-low dose – 1x10-17%. The growth regulator Superstim 2 at the stages of adaptation and subsequent growing is effectively used only in one concentration – 1x10-16%. The additional foliar treatments at the stage of subsequent growing are not necessary. 

scholarly journals Optimization of nutrient media for clonal micropropagation of the rootstock grape variety ‘Kober 5BB’

2020 ◽  
pp. 298-303
Author(s):  
Игорь Владимирович Гавриленко ◽  
Юлия Сергеевна Матяш ◽  
Анжела Владимировна Гавриленко ◽  
Дмитрий Александрович Шанин ◽  
Ирина Александровна Павлова ◽  
...  
Keyword(s):  
Culture Medium ◽  
Test Results ◽  
Grape Variety ◽  
Cultivation Conditions ◽  
Nutrient Media ◽  
Biometric Parameters ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Pathogenic Infection

Сорт винограда Кобер 5 ББ (Берландиери x Рипариа Кобер 5ББ) - один из основных подвоев, используемых в питомниководстве для получения привитых саженцев, поэтому в настоящее время крайне актуально создание маточников данного подвоя посадочным материалом категории «оригинальный». Это объясняет необходимость проведения исследований, связанных с оптимизацией условий культивирования сорта Кобер 5ББ, для повышения эффективности массового клонального микроразмножения с сохранением его генетической однородности и стабильности. Целью исследования являлась оптимизация и подбор питательных сред для клонального микроразмножения сорта-подвоя Кобер 5 ББ на этапе тиражирования (микрочеренкования). Материалом для исследований служили растения in vitro сорта подвоя Кобер 5ББ, свободные от основной патогенной инфекции (по результатам тестирования). Исследования проводили на средах: МS; WPM; DKW; PG (контроль). В качестве регуляторов роста использовали GA (гиббереллиновая кислота) в концентрациях: 0,2; 0,6; 1; 1,4 мг/л в сочетании с NAA (α-нафтилуксусная кислота) 0,05 мг/л. Показано, что растения на среде WPM, содержащей NAA-0,05 мг/л, по биометрическим показателям превосходили развившиеся на среде PG с аналогичным гормональным составом. Проведенные исследования по оптимизации среды культивирования для ускорения ростовых процессов позволили по результатам биометрических показателей выделить блок сред с основой WPM для размножения сорта-подвоя Кобер 5 ББ на этапе микрочеренкования. После проведения дополнительных исследований с расширенной выборкой среду WPM можно будет рекомендовать для клонального микроразмножения винограда на этапе микрочеренкования. The grape variety ‘Kober 5 BB’ (‘Berlandieri x Riparia Kober 5BB’) is one of the main rootstocks used in rootstock-growing farming to obtain grafted seedlings. Currently it is a hot issue to create nurseries for the rootstock grapevine with planting material of the "original" category. This explains the need for research related to optimization of cultivation conditions of the variety ‘Kober 5BB’ to increase the efficiency of mass clonal micropropagation while retaining its genetic homogeny and stability. The aim of the study was to optimize and select nutrient media for clonal micropropagation of the rootstock variety ‘Kober 5 BB’ at the stage of tiraging (micropropagation by cutting). The material of research was the in vitro plants of ‘Kober 5BB’ rootstock variety, free from basic pathogenic infection (according to the test results). The studies were carried out on media: MS; WPM; DKW; PG (control). Gibberellic acid (GA) was used as a growth regulator at concentrations: 0.2; 0.6; 1; 1.4 mg/l in combination with NAA (α-naphthyl acetic acid) 0.05 mg/l. Plants on WPM medium containing NAA-0.05 mg/l in biometric parameters were superior to those grown on PG medium with a similar hormonal composition. According to the results of biometric parameters the studies on optimization the culture medium for accelerating growth processes made it possible to isolate a group of media with a WPM base for propagation of the rootstock variety ‘Kober 5 BB’ at the stage of microcutting. After additional studies with expanded selection, the WPM medium can be recommended for clonal micropropagation of grapes at the stage of microcutting.

scholarly journals Cytokinin Mediated Increased In Vitro Production of Secondary Metabolites with Special Reference to Solasodine in Solanum erianthum

2021 ◽  
Vol 8 (02) ◽  
pp. e62-e68
Author(s):  
Jeeta Sarkar ◽  
Nirmalya Banerjee
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Culture Medium ◽  
In Vitro Production ◽  
Leaf Extracts ◽  
Plant Parts ◽  
Regenerated Plants ◽  
Vitro Production ◽  
Regenerated Plantlets

AbstractSteroid alkaloid solasodine is a nitrogen analogue of diosgenin and has great importance in the production of steroidal medicines. Solanum erianthum D. Don (Solanaceae) is a good source of solasodine. The aim of this study was to evaluate the effect of different cytokinins on the production of secondary metabolites, especially solasodine in the in vitro culture of S. erianthum. For solasodine estimation, field-grown plant parts and in vitro tissues were extracted thrice and subjected to high-performance liquid Chromatography. Quantitative analysis of different secondary metabolites showed that the amount was higher in the in vitro regenerated plantlets compared to callus and field-grown plants. The present study critically evaluates the effect of the type of cytokinin used in the culture medium on solasodine accumulation in regenerated plants. The highest solasodine content (46.78±3.23 mg g-1) was recorded in leaf extracts of the in vitro grown plantlets in the presence of 6-γ,γ-dimethylallylamino purine in the culture medium and the content was 3.8-fold higher compared to the mother plant.

scholarly journals Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

2021 ◽  
pp. 520-528
Author(s):  
Sergey Makarov ◽  
Sergey Rodin ◽  
Irina Kuznetsova ◽  
Anton Chudetsky ◽  
Svetlana Tsaregradskaya
Keyword(s):  
In Vitro Rooting ◽  
Light Emitting ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Medicinal Value ◽  
American Cranberry ◽  
Food Market ◽  
Spectral Ranges

Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

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