Efficient plant regeneration from seedling explants of two commercially important temperate eucalypt species–Eucalyptus nitens and E. globulus

Plant Science ◽  
1999 ◽  
Vol 140 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Saumitra Bandyopadhyay ◽  
Karen Cane ◽  
Gillian Rasmussen ◽  
John D Hamill
Keyword(s):  
Plant Regeneration ◽  
Eucalyptus Nitens ◽  
Eucalypt Species ◽  
Seedling Explants ◽  
Commercially Important

Eucalyptus nitens plant regeneration from seedling explants through direct adventitious shoot bud formation

Trees ◽  
2019 ◽  
Vol 33 (6) ◽  
pp. 1667-1678 ◽  
Author(s):  
Paula G. Ayala ◽  
Elsa A. Brugnoli ◽  
Claudia V. Luna ◽  
Ana M. González ◽  
Raúl Pezzutti ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Adventitious Shoot ◽  
Eucalyptus Nitens ◽  
Bud Formation ◽  
Shoot Bud ◽  
Seedling Explants ◽  
Adventitious Shoot Bud

scholarly journals Cytomorphological studies on somatic embryogenesis of Gentiana tibetica (King) and G. cruciata (L.)

2014 ◽  
Vol 65 (1-2) ◽  
pp. 47-51 ◽  
Author(s):  
Anna Mikuła ◽  
Maria Wesołowska ◽  
Józef Kapusta ◽  
Lutosława Skrzypczak ◽  
Jan J. Rybczyński
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Agar Medium ◽  
Induction Medium ◽  
Mineral Salts ◽  
Fast Green ◽  
Seedling Explants ◽  
Callus Initiation ◽  
Pas Reaction

The process of plant regeneration via somatic embryogenesis of two gentianas, <em>Gentiana tibetica</em> and <em>G. cruciata</em> was described. For this purpose seedling explants were cultured on agar medium and later maintained in cell suspension. For callus initiation seedling explants like: cotyledons, hypocotyl and root were plated on a callus induction medium (CIM) composed of MS (1962), supplemented with 0.5 mg/l 2,4-D and 1.0 mg/l Kin. For the formation of cell suspension culture, embryogenic callus was transferred into liquid maintained medium (MM) composed of MS (1962), supplemented with 1.0 mg/l Dic + 0.1 mg/l NAA + 2.0 mg/l BAP + 80.0 mg/l SA. The conversion of somatic embryo into plantlets required a new medium (ECM) based on MS (1962) mineral salts, supplemented with 0.5 mg/l GA<sub>3</sub> + 1.0 mg/l Kin + 0.5 mg/l NAA. For cytomorphological studies of particular stages of embryogenesis, specimens were stained with dyes and reagents: 1. PAS reaction with leukofucsin, 2. Safranin + fast green, 3. Erlich's hematoxylin.

Pollinators in seed orchards of Eucalyptus nitens (Myrtaceae)

2004 ◽  
Vol 52 (2) ◽  
pp. 209 ◽  
Author(s):  
A. B. Hingston ◽  
P. B. McQuillan ◽  
B. M. Potts
Keyword(s):  
Seed Production ◽  
Common Species ◽  
Eucalyptus Nitens ◽  
Floral Structure ◽  
Seed Orchards ◽  
The World ◽  
Commercially Important ◽  
Time Of Year ◽  
Insect Activity ◽  
Effective Pollinators

Flowers of the commercially important tree Eucalyptus nitens (Deane & Maiden) Maiden were visited by a diverse array of insects, but not by birds, in Tasmanian seed orchards. Most species of insects that visited the flowers of E. nitens are likely to be effective pollinators because all common species of visitors carried many grains of Eucalyptus pollen, and the open floral structure facilitates frequent insect contact with stigmas. Seed production also suggested that a wide variety of insects were effective pollinators because flowers were consistently well pollinated, despite differences in flower-visitor communities among orchards and particular branches of flowers. The generalised entomophilous pollination system of E. nitens suggests that effective pollinators should occur in seed orchards of this tree throughout the world, provided that flowering occurs at a time of year conducive to insect activity. Although a wide variety of insects appear to be effective pollinators of E. nitens, introduced honeybees, Apis mellifera L., that are often deployed as pollinators in seed orchards were consistently not attracted to the flowers. The reliance on wild insects as pollinators suggests that seed production in E. nitens may benefit from reduced use of broad-spectrum insecticides in, and near, seed orchards.

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