The relation between low K+/Na+ ratio and salt-tolerance in the wild tomato species Lycopersicon pennellii

Abstract The tomato-potato psyllid (TPP), Bactericera cockerelli, is a vector for the phloem-limited bacterium Candidatus Liberibacter solanacearum (Lso), the causative agent of economically important diseases including tomato vein-greening and potato zebra chip. Here, we screened 11 wild tomato relatives for TPP resistance as potential resources for tomato (Solanum lycopersicum) cultivar development. Six accessions with strong TPP resistance (survival <10%) were identified within S. habrochaites, S. pennelli, S. huaylasense, S. chmielewskii, S. corneliomulleri, and S. galapagense. Two S. pennelli and S. corneliomulleri accessions also showed resistance to Lso. We evaluated recombinant inbred lines (RILs) carrying resistance from S. habrochaites accession LA1777 in the S. lycopersicum background and identified major quantitative trait loci (QTLs) responsible for adult TPP mortality and fecundity in several RILs carrying insertions in different chromosomes, indicating the polygenic nature of these traits. Analysis of a major resistance QTL in RIL LA3952 on chromosome 8 revealed that the presence of Lso is required to increase adult TPP mortality. By contrast, the reduced TPP oviposition trait in LA3952 is independent of Lso. Therefore, resistance traits are available in wild-tomato species, although their complex inheritance and modes of action require further characterisation to optimise their utilisation for tomato improvement.

Download Full-text

Salinity Tolerance in Four Wild Tomato Species using Vegetative Yield-Salinity Response Curves

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.116.2.286 ◽

1991 ◽

Vol 116 (2) ◽

pp. 286-290 ◽

Cited By ~ 62

Author(s):

M.C. Bolarín ◽

F.G. Fernández ◽

V. Cruz ◽

J. Cuartero

Keyword(s):

Salt Tolerance ◽

Piecewise Linear ◽

Response Threshold ◽

Yield Reduction ◽

Inversion Method ◽

Response Curves ◽

Wild Tomato ◽

Wild Tomato Species ◽

Tomato Species ◽

Salinity Response

The salinity tolerances of 21 accessions belonging to four wild tomato species [Lycopersicon pimpinellifolium (Jusl.) Mill., L. peruvianum (Corr.) D'Arcy, L. hirsutum (L.) Mill., and L. pennellii Humb. Bonpl.) were evaluated using their vegetative yield-salinity response curves at the adult stage, determined by a piecewise-linear response model. The slope (yield decrease per unit salinity increase), salinity response threshold, maximum electrical conductivity without yield reduction (ECo), and salinity level for which yield would be zero (ECo) were determined by a nonlinear least-squares inversion method from curves based on the response of leaf and stem dry weights to substrate EC. The genotype PE-2 (L. pimpinellifolium) had the highest salt tolerance, followed by PE-45 (L. pennellii), PE-34, PE-43 (L. hirsutum), and PE-16 (L. peruvianum). The model also was tested replacing substrate salinity levels with leaf Cl- or Na+ concentrations. Concentrations of both ions for which vegetative yields were zero (Clo and Nao) were determined from the response curves. In general, the most tolerant genotypes were those with the highest Clo and Nao values, suggesting that the dominant salt-tolerance mechanism is ion accumulation, but there were cases in which salt tolerance was not related to Clo and Nao.

Download Full-text

SALT TOLERANCE OF A SELECTED LINE FROM THE CROSS BETWEEN A WILD, TOLERANT TOMATO SPECIES (LYCOPERSICON CHEESMANII, ECOTYPE LA 1401) AND A CULTIVATED SPECIES (L. ESCULENTUM MILL.)

HortScience ◽

10.21273/hortsci.27.6.597a ◽

1992 ◽

Vol 27 (6) ◽

pp. 597a-597 ◽

Cited By ~ 2

Author(s):

Michael C. Shannon ◽

Anne F. Wrona

Keyword(s):

Salt Tolerance ◽

Solution Culture ◽

Parental Line ◽

Wild Tomato Species ◽

Wild Parent ◽

Tomato Species ◽

In The Wild ◽

Cultivated Species ◽

Lycopersicon Cheesmanii ◽

Na Uptake

A salt-tolerance selected F5 generation from a cross between the wild tomato species, Lycopersicon cheesmanii, ecotype LA 1401, and the cultivated species, L. esculentum Mill. (cv Heinz 1350) was compared to the wild parental line in a solution culture experiment to determine the effects of selection on salt tolerance, and ion discrimination and accumulation characteristics in the selected line. Seedlings were transplanted to nutrient solutions at the 3 to 4-leaf stage of growth and after a 1-week period of adjustment, were salinized at 25 mM NaCl day-1 (approximately -1 bar osmotic potential) to final salt concentrations of 0, 50, and 100 mM. Plasmalemma and tonoplast vesicles were isolated from fresh root samples, and ATPase and Na+/H+ antiport activity was determined using fluorescence assays. The selected line restricted Na uptake into the shoot and maintained higher shoot K+ than did the wild parent. Growth rate under salinity was greater in the selected line than in the wild species, but relative salt tolerance was higher in the wild parent. Interspecific hybridization appears to be a useful process for the transfer of salt tolerance characters from wild to cultivated tomato.

Download Full-text