Chlorsulfüron effects on protein synthesis and accumulation in cultured root tips of Pisum sativum L.

1991 ◽  
Vol 137 (3) ◽  
pp. 337-341 ◽  
Author(s):  
David S. Clayton ◽  
Thomas L. Reynolds
Keyword(s):  
Protein Synthesis ◽  
Pisum Sativum ◽  
Root Tips ◽  
Pisum Sativum L

Preparation of pea (Pisum sativum L.) chromosome and nucleus suspensions from single root tips

1996 ◽  
Vol 92 (6) ◽  
pp. 744-751 ◽  
Author(s):  
G. Gualberti ◽  
J. Doležel ◽  
J. Macas ◽  
S. Lucretti
Keyword(s):  
Pisum Sativum ◽  
Root Tips ◽  
Single Root ◽  
Pisum Sativum L

Preparation of pea (Pisum sativum? L.) chromosome and nucleus suspensions from single root tips

1996 ◽  
Vol 92 (6) ◽  
pp. 744-751 ◽  
Author(s):  
G. Gualberti ◽  
J. Dolezel ◽  
J. Macas ◽  
J. Macas ◽  
S. Lucretti ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Root Tips ◽  
Single Root ◽  
Pisum Sativum L

scholarly journals Protein synthesis in the cotyledons of Pisum sativum L. Protein factors involved in the binding of phenylalanyl-transfer ribonucleic acid to ribosomes*

1974 ◽  
Vol 139 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Gary N. Wells ◽  
Leonard Beevers
Keyword(s):  
Protein Synthesis ◽  
Complex Formation ◽  
Pisum Sativum ◽  
Ribonucleic Acid ◽  
Ternary Complex ◽  
Supernatant Fraction ◽  
Soluble Factors ◽  
Pisum Sativum L ◽  
Sensitive Site ◽  
Millipore Filters

1. Proteinaceous factors contained in a 0.5m-KCl extract of ribosomes from pea cotyledons form a ternary complex at 0°C with [14C]phenylalanyl-tRNA and poly(U). The complex is measured by its quantitative retention on Millipore filters. 2. Complex-assembly is optimal at 5mm-Mg2+ and is independent of GTP and ribosomes. 3. The addition of ribosomes is required to stabilize the complex at 34°C. The complex binds to a puromycin-sensitive site on the ribosome. 4. Soluble factors from the 250000g supernatant of pea cotyledon form a Millipore-retainable complex dependent on GTP and ribosomes. 5. Complex-formation by soluble factors has a Mg2+ optimum of 10–12mm and forms a puromycin-insensitive complex with ribosomes. 6. The function of the ribosomal protein factors and the supernatant fraction in initiation of protein synthesis is discussed.

Long-lived Messenger RNA and its Relationship to Protein Synthesis During Germination of Pea (Pisum sativum L.) Seeds

1986 ◽  
Vol 57 (6) ◽  
pp. 771-781 ◽  
Author(s):  
S. C. Greenway ◽  
G. M. Strangeway ◽  
D. Grierson ◽  
J. A. Bryant
Keyword(s):  
Protein Synthesis ◽  
Pisum Sativum ◽  
Messenger Rna ◽  
Pisum Sativum L

scholarly journals Chromosomal Aberrations Induced by Carbaryl in Root Meristem Cells of Pisum Sativum L.

2017 ◽  
Vol 14 (3) ◽  
pp. 985-987
Author(s):  
Khushboo Kushwah ◽  
Ravendra Singh Chauhan ◽  
R.K. Sarbhoy ◽  
Harshal Kumar
Keyword(s):  
Cell Division ◽  
Pisum Sativum ◽  
Somatic Cell ◽  
Chromosomal Aberrations ◽  
Filter Paper ◽  
Root Meristem ◽  
Distilled Water ◽  
Root Tips ◽  
Uniform Size ◽  
Pisum Sativum L

ABSTRACT: Carbaryl, which is also known as sevin, induced mitostatic and turbagenic leading to clastogenic effects in the root meristem cells of Pisum sativum. The study was conducted at Department of Botany, Agra College, Agra. Seeds of uniform size of Pisum sativum were germinated on moist filter paper in petriplates. 1to 2 mm root tips were cut and treated with different concentrations (0.1, 0.2, 0.3,0.5%) of carbaryl prepared in distilled water for varying duration (3 to 9 hrs.) of time. It has mitodepressive and mitostatic effects on somatic cell division. These effects are directly proportional to concentration and duration. Common clastogenic effects are stickiness, condensation, breakage and bridges etc. Present investigation clearly revealed that carbaryl showed clastogenic and mitostatic effects. So, it should be used with precautions as it can be hazardous to both targeted and non-targeted biota.

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