Protonation-Dependent Sequencing of 5-Formylcytidine in RNA

Chemical modification of cytidine in non-coding RNAs plays a key role in regulating translation and disease. However, the distribution and dynamics of many of these modifications remains unknown due to a lack of sensitive site-specific sequencing technologies. Here we report a protonation-dependent sequencing reaction for detection of 5-formylcytidine (5fC) and 5-carboxycytidine (5caC) in RNA. First, we evaluate how protonation combined with electron-withdrawing substituents alters the molecular orbital energies and reduction of modified cytidine nucleosides, highlighting 5fC and 5caC as reactive species. Next, we apply this reaction to detect these modifications in synthetic oligonucleotides as well as endogenous human tRNA. Finally, we demonstrate the utility of our method to characterize a patient-derived model of 5fC-deficiency, where it enables facile monitoring of both pathogenic loss and exogenous rescue of NSUN3-dependent 5fC within the wobble base of human mitochondrial tRNAMet. These studies showcase the ability of protonation to enhance the reactivity and sensitive detection of 5fC in RNA, and provide a molecular foundation for applying optimized sequencing reactions to better understand the role of oxidized RNA cytidine nucleobases in disease.

DEVELOPMENT OF RIG HANGING HYDRO TURBINE BASED ON WASTE WATER DISCHARGE AT CHALLAWA INDUSTRIAL AREA OF KANO STATE

Design of hydroelectricity system is employed here in an effort to produce electric energy using an exhaust waste water of steam power plant. The major concern in this work is how the system is designed, selection of site and resources. The system after designed and fabricated was mounted at waste water outlet for proof run for about three months period. The result obtained exhibit the overall design is feasible for the sensitive site application

Evolution of Contingency Planning in California – a Federal and State Coordinated Effort

ABSTRACT U.S. Coast Guard (USCG), U.S. Environmental Protection Agency (USEPA), and California Department of Fish and Wildlife (CDFW), Office of Spill Prevention and Response (OSPR) agreed to the joint preparation of oil spill contingency plans following the enactment of California and federal laws in 1990. With little guidance initially, six Area Committees embarked on a new process to create the first coastal Area Contingency Plans (ACPs) in 1992. In 2015, when emergency regulations to expand OSPR's jurisdiction statewide became effective, developing inland Geographic Response Plans (GRPs) became a top priority. Over the last 30 years, the ACPs have evolved into robust, nationally recognized planning documents, and response plans have expanded into inland environments. This paper will describe in detail the contributions and advancements in California oil spill contingency planning over 30 years. It will highlight OSPR's approaches for statewide consistency and coverage, interoperability between various state and federal plans, tools (including Geographic Information System (GIS) and various databases), improved sensitive site contacts and management, and lessons learned. It will also cover goals for future improvements in both marine and inland contingency plans.

California's Response to USCG Nationwide Standardization of Area Contingency Plans

ABSTRACT California Department of Fish and Wildlife (CDFW) - Office of Spill Prevention and Response (OSPR), working with the United States Coast Guard (USCG), and Area Committee members, made significant strides to streamline the Area Contingency Plans (ACPs) for improved efficiency and statewide consistency to adhere to new USCG guidance. Beginning with Sector San Diego's ACP, which underwent major revision in 2018, and Sector Los Angeles/Long Beach in 2019, OSPR worked closely with USCG to ensure that there is comparable information statewide, improved maps and GIS compatibility, and updated environmentally and economically sensitive site information. OSPR created a new environmental sensitive site database, including more user-friendly Geographic Response Strategy pages for those identified sites. OSPR also revised the content of Section 9800, which describes the environmental, cultural, historic, and economic sensitivities of a given ACP area, and includes the Geographic Response Strategies. This paper describes in detail the contributions and changes that OSPR has made to California ACPs since 2018. It highlights its approaches to streamlining for efficiency and statewide consistency and lessons learned from the new revision and approval processes.

Influence of Boron Priming on the Antioxidant Ability of Alfalfa Seeds

This experiment was designed to determine the relationship between the antioxidant capacity and the vigour of alfalfa seeds during boron priming. Alfalfa seeds were primed with 1.8 % (W/V) concentration of borax solution for 0, 3, 6, 12 and 24 h at 20°C. The results showed that the vigour of alfalfa seeds declined with the extension of priming time, which was closely related to the accumulation of hydrogen peroxide and malonaldehyde contents and the decrease of superoxide dismutase, catalase and peroxidase. The radicle might be the most sensitive site to boron priming in alfalfa seeds, but the boron-induced damage might be alleviated during alfalfa seeds’ germination.

A molecular switch for Cdc48 activity and localization during oxidative stress and aging

SummaryControl over a healthy proteome begins with the birth of the polypeptide chain and ends with coordinated protein degradation. One of the major players in eukaryotic protein degradation is the essential and highly conserved ATPase, Cdc48 (p97/VCP in mammals). Cdc48 mediates clearance of misfolded proteins from the nucleus, cytosol, ER, mitochondria, and more. Here we dissect the crosstalk between cellular oxidation and Cdc48 activity by identification of a redox-sensitive site, Cys115. By integrating proteomics, biochemistry, microscopy, and bioinformatics, we show that removal of Cys115’s redox-sensitive thiol group leads to accumulation of Cdc48 in the nucleus and consequently, results in severe defects in the oxidative stress response, mitochondrial fragmentation, and a decrease in ERAD and sterol biogenesis. We have thus identified a unique redox switch in Cdc48, which may provide a clearer picture of the importance of Cdc48’s localization in maintaining a “healthy” proteome during oxidative stress and chronological aging in yeast.