Plant Regeneration from Long-Term Cell Suspension Cultures of Indica Rice (Oryza sativa L. cv. IR43)

1992 ◽  
Vol 139 (5) ◽  
pp. 523-527 ◽  
Author(s):  
G.C. Ghosh Biswas ◽  
F.J. Zapata
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals Genetic Analysis of Plant Regeneration Ability in Cell Suspension Cultures of Rice (Oryza sativa L.).

1995 ◽  
Vol 45 (4) ◽  
pp. 425-428
Author(s):  
Masayoshi Tsukahara ◽  
Takayasu Hirosawa ◽  
Eiko Nagai ◽  
Hiroshi Kato ◽  
Ryorchi Ikeda ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Genetic Analysis ◽  
Cell Suspension ◽  
Oryza Sativa L ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regeneration Ability

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

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