The relation of potato leaf roll virus net necrosis in potato tubers to the interval between planting and inoculation

1997 ◽  
Vol 16 (6) ◽  
pp. 533-539 ◽  
Author(s):  
Jutta Roosen ◽  
Ray G. Huffaker ◽  
Raymond J. Folwell ◽  
Thomas L. Marsh ◽  
Ronald C. Mittelhammer
2016 ◽  
Vol 145 (4) ◽  
pp. 829-845 ◽  
Author(s):  
Anja Hühnlein ◽  
Jörg Schubert ◽  
Volker Zahn ◽  
Thomas Thieme

2014 ◽  
Vol 94 (1) ◽  
pp. 1-7
Author(s):  
Almouner A.A. Yattara ◽  
Amadou K. Coulibaly ◽  
Frédéric Francis

Des études sur l’abondance et la diversité des pucerons ont été menées pendant trois campagnes agricoles au Mali. Sur la base de relevés de bacs jaunes installés dans des cultures de pomme de terre à Kati et à Sikasso, 2 525 pucerons ont été capturés et identifiés. Dix-neuf espèces de pucerons ont été recensées, dont deux qui ont été observéesin situsur la culture :Aphis gossypii(Glover) etMyzus persicae(Sulzer). La plupart de ces espèces sont des ravageurs de cultures et elles contribuent également à la transmission virale. Des échantillons foliaires prélevés dans des parcelles de pomme de terre dans les deux régions ont été testés par la technique ELISA pour la détection des deux principaux virus dommageables, soit lePotato VirusY (PVY) et lePotato Leaf Roll Virus(PLRV). Le taux de plantes virosées dans les deux localités pendant les trois années variait de 19,3 % à 21,8 % pour le PVY, alors qu’il était de 8,5 % à 9,3 % pour le PLRV. L’occurrence de ces maladies virales s’est révélée être très homogène d’une année à l’autre, avec des taux relativement importants. Cette étude est une première quantification dans cette région du Mali de l’importance des relations pucerons vecteurs–virus en culture de pomme de terre.


2018 ◽  
Vol 73 (11-12) ◽  
pp. 423-438 ◽  
Author(s):  
Engy E. Abdel Aleem ◽  
Radwa M. Taha ◽  
Faiza A. Fattouh

Abstract Solanum tuberosum (potato) is the second most important vegetable crop in Egypt. It is locally consumed, manufactured or supplied for export to Europe and other Arab countries. Potato is subject to infection by a number of plant viruses, which affect its yield and quality. Potato virus Y (PVY), potato leaf roll virus (PLRV), and Alfalfa mosaic virus (AMV) were detected in major potato-growing areas surveyed. Multiplex-RT-PCR assay was used for the detection of these three viruses in one reaction using three specific primer pairs designed to amplify genomic parts of each virus (1594 bp for PLRV, 795 bp for AMV, 801 bp for PVY). All three viruses were detected in a single reaction mixture in naturally infected field-grown potatoes. Multiplex RT-PCR improved sensitivity necessary for the early detection of infection. Incidence of single, double, or triple infection has been recorded in some locations. Full-length sequencing has been performed for an Egyptian FER isolate of PLRV. Through phylogenetic analysis, it was shown to occupy the same clade with isolate JokerMV10 from Germany. Complete nucleotide sequence of an Egyptian FER isolate of AMV and phylogenetic analysis was also performed; we propose that it is a new distinct strain of AMV belonging to a new subgroup IIC. This is the first complete nucleotide sequence of an Egyptian isolate of AMV. Genetic biodiversity of devastating potato viruses necessitates continuous monitoring of new genetic variants of such viruses.


1944 ◽  
Vol 22c (4) ◽  
pp. 162-170 ◽  
Author(s):  
G. B. Sanford ◽  
J. G. Grimble

The results of field tests with random lots of potato tubers showing phloem necrosis of similar type are summarized. The vines from tubers of five of these developed typical leaf-roll symptoms, but those of three others showed no sign of leaf roll, or other symptoms that suggested a virus as the causal agent. The net necrosis in one of the latter samples was known to result from vine infestation of Paratrioza cockerelli Sulc, and in this case the stock returned to full vigour during the second season. It was further confirmed that once the vines show definite symptoms of leaf roll, the phloem elements of their tubers henceforth do not exhibit necrosis.The tissue contiguous to the necrotic phloem elements in the affected tubers was uniformly fluorescent, but the unaffected portion of such tubers, as well as non-necrotic tubers from positive leaf-roll plants, reacted negatively to ultraviolet light.


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