scholarly journals Intranasal Immunization of Mice with Group B Streptococcal Protein Rib and Cholera Toxin B Subunit Confers Protection against Lethal Infection

2004 ◽  
Vol 72 (2) ◽  
pp. 1184-1187 ◽  
Author(s):  
Charlotte Larsson ◽  
Jan Holmgren ◽  
Gunnar Lindahl ◽  
Charlotta Bergquist
Keyword(s):  
Cholera Toxin ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit ◽  
A Cell ◽  
Group B

ABSTRACT Intranasal immunization of mice with Rib, a cell surface protein of group B streptococcus (GBS), conjugated to or simply coadministered with the recombinant cholera toxin B subunit, induces systemic immunoglobulin G (IgG) and local IgA antibody responses and confers protection against lethal GBS infection. These findings have implications for the development of a human GBS vaccine.

Preparation and preclinical evaluation of experimental group B streptococcus type III polysaccharide–cholera toxin B subunit conjugate vaccine for intranasal immunization

Vaccine ◽  
2000 ◽  
Vol 19 (7-8) ◽  
pp. 850-861 ◽  
Author(s):  
Xuzhuang Shen ◽  
Teresa Lagergård ◽  
Yonghong Yang ◽  
Marianne Lindblad ◽  
Margareta Fredriksson ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Preclinical Evaluation ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Group B ◽  
Experimental Group

scholarly journals Group B Streptococcus Capsular Polysaccharide-Cholera Toxin B Subunit Conjugate Vaccines Prepared by Different Methods for Intranasal Immunization

2001 ◽  
Vol 69 (1) ◽  
pp. 297-306 ◽  
Author(s):  
Xuzhuang Shen ◽  
Teresa Lagergård ◽  
Yonghong Yang ◽  
Marianne Lindblad ◽  
Margareta Fredriksson ◽  
...  
Keyword(s):  
Cholera Toxin ◽  
Capsular Polysaccharide ◽  
Cholera Toxin B Subunit ◽  
Small Molecular Weight ◽  
Toxin B ◽  
Conjugate Vaccines ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Group B

ABSTRACT Group B Streptococcus (GBS) type III capsular polysaccharide (CPS III) was conjugated to recombinant cholera toxin B subunit (rCTB) using three different methods which employed (i) cystamine and N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), (ii) carbodiimide with adipic acid dihydrazide (ADH) as a spacer, or (iii) reductive amination (RA). The CPS III-rCTB conjugates were divided into large- and small-molecular-weight (M r) fractions, and the immunogenicities of the different preparations after intranasal (i.n.) immunization were studied in mice. Both large- and small-M rconjugates of CPS III-rCTBRA or CPS III-rCTBADHinduced high, almost comparable levels of CPS-specific immunoglobulin G (IgG) in serum, lungs, and vagina that were generally superior to those obtained with CPS III-rCTBSPDP conjugates or a CPS III and rCTB mixture. However, the smaller-M rconjugates of CPS III-rCTBRA or CPS III-rCTBADHin most cases elicited a lower anti-CPS IgA immune response than the large-M r conjugates, and the highest anti-CPS IgA titers in both tissues and serum were obtained with the large-M r CPS III-rCTBRA conjugate. Serum IgG anti-CPS titers induced by the CPS III-rCTBRAconjugate had high levels of specific IgG1, IgG2a, IgG2b, and IgG3 antibodies. Based on the effectiveness of RA for coupling CPS III to rCTB, RA was also tested for conjugating GBS CPS Ia with rCTB. As for the CPS III-rCTB conjugates, the immunogenicity of CPS Ia was greatly increased by conjugation to rCTB. Intranasal immunization with a combination of CPS Ia-rCTB and CPS III-rCTB conjugates was shown to induce anti-CPS Ia and III immune responses in serum and lungs that were fully comparable with the responses to immunization with the monovalent CPS Ia-rCTB or CPS III-rCTB conjugates. These results suggest that the GBS CPS III-rCTB and CPS Ia-rCTB conjugates prepared by the RA method may be used in bivalent and possibly also in multivalent mucosal GBS conjugate vaccines.

scholarly journals Intranasal Immunization with the Cholera Toxin B Subunit-Pneumococcal Surface Antigen A Fusion Protein Induces Protection against Colonization with Streptococcus pneumoniae and Has Negligible Impact on the Nasopharyngeal and Oral Microbiota of Mice

2006 ◽  
Vol 74 (8) ◽  
pp. 4939-4944 ◽  
Author(s):  
F. C. Pimenta ◽  
E. N. Miyaji ◽  
A. P. M. Arêas ◽  
M. L. S. Oliveira ◽  
A. L. S. S. de Andrade ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Fusion Protein ◽  
Cholera Toxin ◽  
Surface Antigen ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Antigen A

ABSTRACT One of the candidate proteins for a mucosal vaccine antigen against Streptococcus pneumoniae is PsaA (pneumococcal surface antigen A). Vaccines targeting mucosal immunity may raise concerns as to possible alterations in the normal microbiota, especially in the case of PsaA, which was shown to have homologs with elevated sequence identity in other viridans group streptococci. In this work, we demonstrate that intranasal immunization with a cholera toxin B subunit-PsaA fusion protein is able to protect mice against colonization with S. pneumoniae but does not significantly alter the natural oral or nasopharyngeal microbiota of mice.

A comparison of natural and recombinant cholera toxin B subunit as stimulatory factors in intranasal immunization

Vaccine ◽  
1997 ◽  
Vol 15 (10) ◽  
pp. 1110-1113 ◽  
Author(s):  
Bernard de Geus ◽  
Maike Dol-Bosman ◽  
Jan Willem Scholten ◽  
Wil Stok ◽  
Andre Bianchi
Keyword(s):  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit

scholarly journals Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization

2010 ◽  
Vol 78 (9) ◽  
pp. 3773-3782 ◽  
Author(s):  
Takeshi Miyata ◽  
Tetsuya Harakuni ◽  
Takafumi Tsuboi ◽  
Jetsumon Sattabongkot ◽  
Hideyasu Kohama ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Cholera Toxin ◽  
Surface Protein ◽  
Vaccine Antigen ◽  
Primary Amines ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Transmission Blocking ◽  
Cholera Toxin B ◽  
B Subunit

ABSTRACT The nontoxic cholera toxin B subunit (CTB) was evaluated as a potential delivery molecule for the Plasmodium vivax ookinete surface protein, Pvs25. Recombinant Pvs25 was expressed as a secreted protein in the yeast Pichia pastoris, as a mixture of isoforms including multimers and the A and B monomers. The A isoform with the presumed native protein fold was the most abundant, accounting for more than 40% of all expressed protein. The molecularly uniform A isoform was chemically conjugated to CTB via its primary amines, and the fusion protein, retaining GM1-ganglioside affinity, was administered to BALB/c mice by the subcutaneous (s.c.) or intranasal (i.n.) route. Immunization of mice with conjugated Pvs25 without supplemental adjuvant induced antisera that specifically recognized P. vivax ookinetes in vitro. Furthermore, the antisera, when mixed with parasitized blood isolated from P. vivax patients from Thailand, was found to reduce parasite transmission to mosquitoes, conferring a 93 to 98% (s.c.) or a 73 to 88% (i.n.) decrease in oocyst number. Unconjugated Pvs25 alone conferred only a 23 to 60% (s.c.) or a 0 to 6% (i.n.) decrease in oocyst number. Coadministration of extraneous adjuvants, however, further enhanced the vaccine efficacy up to complete blockade. Taken together, we conclude that a weakly immunogenic Pvs25 by itself, when linked to CTB, transforms into a potent transmission-blocking antigen in both i.n. and s.c. routes. In addition, the present study is, to the best of our knowledge, the first demonstration of the immune potentiating function of CTB for a vaccine antigen delivered by the s.c. route.

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