scholarly journals Intranasal Immunization with the Cholera Toxin B Subunit-Pneumococcal Surface Antigen A Fusion Protein Induces Protection against Colonization with Streptococcus pneumoniae and Has Negligible Impact on the Nasopharyngeal and Oral Microbiota of Mice

2006 ◽  
Vol 74 (8) ◽  
pp. 4939-4944 ◽  
Author(s):  
F. C. Pimenta ◽  
E. N. Miyaji ◽  
A. P. M. Arêas ◽  
M. L. S. Oliveira ◽  
A. L. S. S. de Andrade ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Fusion Protein ◽  
Cholera Toxin ◽  
Surface Antigen ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Antigen A

ABSTRACT One of the candidate proteins for a mucosal vaccine antigen against Streptococcus pneumoniae is PsaA (pneumococcal surface antigen A). Vaccines targeting mucosal immunity may raise concerns as to possible alterations in the normal microbiota, especially in the case of PsaA, which was shown to have homologs with elevated sequence identity in other viridans group streptococci. In this work, we demonstrate that intranasal immunization with a cholera toxin B subunit-PsaA fusion protein is able to protect mice against colonization with S. pneumoniae but does not significantly alter the natural oral or nasopharyngeal microbiota of mice.

Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants

2005 ◽  
Vol 25 (5) ◽  
pp. 417-424 ◽  
Author(s):  
Dora Li ◽  
Jennifer O’Leary ◽  
Yan Huang ◽  
Norman P. A. Huner ◽  
Anthony M. Jevnikar ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cholera Toxin ◽  
Transgenic Tobacco ◽  
Human Insulin ◽  
Cholera Toxin B Subunit ◽  
Transgenic Tobacco Plants ◽  
Toxin B ◽  
Tobacco Plants ◽  
Cholera Toxin B ◽  
B Subunit

scholarly journals Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects againstBordetella pertussisInfection

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Noelia Olivera ◽  
Celina E. Castuma ◽  
Daniela Hozbor ◽  
María E. Gaillard ◽  
Martín Rumbo ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cholera Toxin ◽  
Bordetella Pertussis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cholera Toxin B Subunit ◽  
Mrna Levels ◽  
Toxin B ◽  
Coding Sequence ◽  
Cholera Toxin B ◽  
B Subunit

This study examined the immunogenic properties of the fusion protein fimbria 2 ofBordetella pertussis(Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this endB. pertussisFim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence. The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P<0.01orP<0.001,resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen againstBordetella pertussisinfection.

scholarly journals Multiserotype Protection of Mice against Pneumococcal Colonization of the Nasopharynx and Middle Ear by Killed Nonencapsulated Cells Given Intranasally with a Nontoxic Adjuvant

2004 ◽  
Vol 72 (7) ◽  
pp. 4290-4292 ◽  
Author(s):  
Richard Malley ◽  
Sarah C. Morse ◽  
Luciana C. C. Leite ◽  
Ana Paula Mattos Areas ◽  
Paulo Lee Ho ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Cholera Toxin ◽  
Middle Ear ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Pneumococcal Colonization

ABSTRACT Intranasal challenge of C57BL/6 mice with Streptococcus pneumoniae serotypes 6B, 14, and 23F produced colonization of the middle ear and NP. Intranasal vaccination with ethanol-killed nonencapsulated cells with adjuvant protected both sites. Of four nontoxic adjuvants tested, the cholera toxin B subunit was most effective and least nonspecifically protective.

scholarly journals Intranasal Immunization of Mice with Group B Streptococcal Protein Rib and Cholera Toxin B Subunit Confers Protection against Lethal Infection

2004 ◽  
Vol 72 (2) ◽  
pp. 1184-1187 ◽  
Author(s):  
Charlotte Larsson ◽  
Jan Holmgren ◽  
Gunnar Lindahl ◽  
Charlotta Bergquist
Keyword(s):  
Cholera Toxin ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit ◽  
A Cell ◽  
Group B

ABSTRACT Intranasal immunization of mice with Rib, a cell surface protein of group B streptococcus (GBS), conjugated to or simply coadministered with the recombinant cholera toxin B subunit, induces systemic immunoglobulin G (IgG) and local IgA antibody responses and confers protection against lethal GBS infection. These findings have implications for the development of a human GBS vaccine.

Gene fusion of cholera toxin B subunit and HBV PreS2 epitope and the antigenicity of fusion protein

Vaccine ◽  
1995 ◽  
Vol 13 (10) ◽  
pp. 933-937 ◽  
Author(s):  
Shi Cheng-hua ◽  
Cao Cheng ◽  
Zhig Jing-sheng ◽  
Li Jiezhi ◽  
Ma Qing-jun
Keyword(s):  
Fusion Protein ◽  
Cholera Toxin ◽  
Gene Fusion ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit
Sign in / Sign up

Export Citation Format

Share Document