In vitro assay of hydrolysis and chlorohydroxy derivatives of bisphenol A diglycidyl ether for estrogenic activity

Novel phosphate triester derivatives of the antiviral drug araA and the anti-leukaemic agent araC have been prepared as membrane-soluble pro-drugs of the bio-active free nucleotides. In particular, novel trichloro- and trifluoroethyl phosphates have been prepared using phosphorochloridate chemistry, and are fully characterized. An in vitro assay indicates inhibition, by each of the compounds, of thymidine incor-portion by mammalian epithelial cells. It is notable that the trichloroethyl derivative is most active in each case, and in the case of araC its activity appears to exceed that of the parent nucleoside.

Download Full-text

Evaluation of Estrogenic Activity in Diets for Experimental Animals Using in Vitro Assay

Journal of Agricultural and Food Chemistry ◽

10.1021/jf034896d ◽

2004 ◽

Vol 52 (5) ◽

pp. 1410-1414 ◽

Cited By ~ 13

Author(s):

Hideo Kato ◽

Toshio Iwata ◽

Yoshinao Katsu ◽

Hajime Watanabe ◽

Yasuhiko Ohta ◽

...

Keyword(s):

Estrogenic Activity ◽

In Vitro Assay ◽

Vitro Assay ◽

Experimental Animals

Download Full-text

Cytotoxic effects of BADGE (bisphenol A diglycidyl ether) and BFDGE (bisphenol F diglycidyl ether) on Caco-2 cells in vitro

Archives of Toxicology ◽

10.1007/s00204-006-0121-1 ◽

2006 ◽

Vol 80 (11) ◽

pp. 748-755 ◽

Cited By ~ 32

Author(s):

Graciela Ramilo ◽

Iago Valverde ◽

Jorge Lago ◽

Juan M. Vieites ◽

Ana G. Cabado

Keyword(s):

Bisphenol A ◽

Diglycidyl Ether ◽

Cytotoxic Effects ◽

Bisphenol F ◽

Bisphenol A Diglycidyl Ether

Download Full-text

A direct in vitro assay for evaluation of diphtheria vaccine efficacy. Measurement of the competition of toxin and toxoids for neutralizing antibodies

Immunology Letters ◽

10.1016/s0165-2478(97)88020-0 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 293-294

Author(s):

A Wahby

Keyword(s):

Vaccine Efficacy ◽

Neutralizing Antibodies ◽

In Vitro Assay ◽

Vitro Assay

Download Full-text

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651281 ◽

1968 ◽

Vol 20 (03/04) ◽

pp. 384-396 ◽

Cited By ~ 1

Author(s):

G Zbinden ◽

S Tomlin

Keyword(s):

Platelet Adhesion ◽

Sodium Citrate ◽

Blood Platelets ◽

In Vitro Assay ◽

Red Cells ◽

Platelet Adhesiveness ◽

Vitro Assay ◽

In Vitro System ◽

Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Download Full-text