Application of immobilized enzymes to clinical analyses : use of co-immobilized glucose oxidase and peroxidase in column form

Biochimie ◽  
1980 ◽  
Vol 62 (8-9) ◽  
pp. 581-585 ◽  
Author(s):  
Takashi Murachi ◽  
Yoshiaki Sakaguchi ◽  
Masayoshi Tabata ◽  
Megumi Sugahara ◽  
Jiro Endo
Keyword(s):  
Glucose Oxidase ◽  
Immobilized Enzymes ◽  
Immobilized Glucose Oxidase

scholarly journals Development of antimicrobial packaging materials with immobilized glucose oxidase and lysozyme

2013 ◽  
Vol 11 (7) ◽  
pp. 1066-1078 ◽  
Author(s):  
Kristýna Hanušová ◽  
Lukáš Vápenka ◽  
Jaroslav Dobiáš ◽  
Linda Mišková
Keyword(s):  
Glucose Oxidase ◽  
Specific Activity ◽  
Immobilized Enzymes ◽  
Lactobacillus Helveticus ◽  
Listeria Ivanovii ◽  
Agar Media ◽  
Ft Ir ◽  
Immobilized Glucose Oxidase ◽  
And Storage ◽  
Ft Ir Spectroscopy

AbstractPackaging based on immobilization of antimicrobial enzymes provides a promising form of active packaging systems applicable in food processing. Glucose oxidase and lysozyme were immobilized by the Ugi reaction with cyclohexyl isocyanide and glutaraldehyde on polyamide and ionomer films partially hydrolysed by hydrochloric acid. The immobilization of the enzymes on the surface of films was confirmed by FT-IR spectroscopy and the films were characterized by the specific activity of the immobilized enzymes. The enzyme migration into model solutions and the effect of pH, temperature and storage time on the activity of immobilized enzyme were also evaluated. Immobilization of lysozyme onto polyamide and ionomer films resulted in the loss of enzyme activity. The polyamide and ionomer films with immobilized glucose oxidase inhibited the growth of bacteria Escherichia coli CNCTC 6859, Pseudomonas fluorescens CNCTC 5793, Lactobacillus helveticus CH-1, Listeria ivanovii CCM 5884 and Listeria innocua CCM 4030 on agar media.

Measurement of glucose in plasma, with use of immobilized glucose oxidase and peroxidase.

1976 ◽  
Vol 22 (8) ◽  
pp. 1378-1382 ◽  
Author(s):  
S W Kiang ◽  
J W Kuan ◽  
S S Kuan ◽  
G G Guilbault
Keyword(s):  
Uric Acid ◽  
Blood Plasma ◽  
Glucose Oxidase ◽  
Immobilized Enzymes ◽  
Magnetic Stirrer ◽  
Immobilized Glucose Oxidase

Abstract We describe a novel fluorometric technique for simple, rapid, and economical assay of glucose by use of immobilized glucose oxidase and peroxidase. A cylindrical magnetic stirrer was specially designed to hold the immobilized enzymes firmly and to allow the reaction minzymic transformation quickly. Blood plasma can be assayed directly with no pretreatment. Ascrobic acid, uric acid, and creatinine in concenctrations of 0.25, 0.25, and 0.2 g/liter, respectively, did not interfere. The linearity of the assay was extended to 4.0 g of glucose concentrations. Results by our technique and by the otoluidine or hexokinase methods agreed well. The immobilized enzymes are stable for several months and can be used for several hundred highly accurate and reproducible assays.

Gold nanoparticles-coated eggshell membrane with immobilized glucose oxidase for fabrication of glucose biosensor

2011 ◽  
Vol 152 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Baozhan Zheng ◽  
Shunping Xie ◽  
Lei Qian ◽  
Hongyan Yuan ◽  
Dan Xiao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Glucose Oxidase ◽  
Glucose Biosensor ◽  
Eggshell Membrane ◽  
Immobilized Glucose Oxidase

Colorimetric Glucose Assay Based on Magnetic Particles Having Pseudo-peroxidase Activity and Immobilized Glucose Oxidase

2016 ◽  
Vol 58 (5) ◽  
pp. 373-380 ◽  
Author(s):  
Pavla Martinkova ◽  
Radka Opatrilova ◽  
Peter Kruzliak ◽  
Igor Styriak ◽  
Miroslav Pohanka
Keyword(s):  
Glucose Oxidase ◽  
Peroxidase Activity ◽  
Magnetic Particles ◽  
Glucose Assay ◽  
Immobilized Glucose Oxidase

Improved enzyme sensor for glucose with an ultrafiltration membrane and immobilized glucose oxidase

1980 ◽  
Vol 116 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Masao Koyama ◽  
Yuichi Sato ◽  
Masuo Aizawa ◽  
Shuichi Suzuki
Keyword(s):  
Glucose Oxidase ◽  
Ultrafiltration Membrane ◽  
Enzyme Sensor ◽  
Immobilized Glucose Oxidase

scholarly journals Development of amperometric enzyme biosensor based on carbon fibre electrode and immobilized glucose oxidase

2003 ◽  
Vol 19 (1) ◽  
pp. 76-80 ◽  
Author(s):  
L. V. Danyleyko ◽  
O. N. Schuvailo ◽  
V. M. Arkhypova ◽  
A. P. Soldatkin ◽  
S. V. Dzyadevych
Keyword(s):  
Carbon Fibre ◽  
Glucose Oxidase ◽  
Enzyme Biosensor ◽  
Immobilized Glucose Oxidase
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