Three immobilized enzymes acting in series in layer by layer assemblies: Exploiting the trehalase-glucose oxidase-horseradish peroxidase cascade reactions for the optical determination of trehalose

2014 ◽  
Vol 202 ◽  
pp. 217-223 ◽  
Author(s):  
Gerardo Palazzo ◽  
Giuseppe Colafemmina ◽  
Claudia Guzzoni Iudice ◽  
Antonia Mallardi
Keyword(s):  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Immobilized Enzymes ◽  
Cascade Reactions ◽  
Layer By Layer ◽  
In Series ◽  
Optical Determination

scholarly journals Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides

1974 ◽  
Vol 137 (1) ◽  
pp. 25-32 ◽  
Author(s):  
D. J. Inman ◽  
W. E. Hornby
Keyword(s):  
Glucose Oxidase ◽  
Enzyme System ◽  
Inside Surface ◽  
Enzyme Systems ◽  
Nylon Tube ◽  
In Series ◽  
Automated Determination

1. Glucose oxidase (EC 1.1.3.4), amyloglucosidase (EC 3.2.1.3), invertase (EC 3.2.1.26) and β-galactosidase (EC 3.2.1.23) were covalently attached via glutaraldehyde to the inside surface of nylon tube. 2. The linked enzyme system, comprising invertase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of sucrose. 3. The linked enzyme system, comprising β-galactosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of lactose. 4. The linked enzyme system, comprising amyloglucosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of maltose. 5. Mixtures of glucose oxidase and amyloglucosidase were immobilized within the same piece of nylon tube and used for the automated determination of maltose. 6. Mixtures of glucose oxidase and invertase were immobilized within the same piece of nylon tube and used for the automated determination of sucrose.

Single- and coupled-enzyme nylon-tube reactors for plasma glucose determination with glucose oxidase and aldehyde dehydrogenase.

1980 ◽  
Vol 26 (12) ◽  
pp. 1652-1655 ◽  
Author(s):  
W Hinsch ◽  
A Antonijewić ◽  
P V Sundaram
Keyword(s):  
Glucose Oxidase ◽  
Aldehyde Dehydrogenase ◽  
Plasma Glucose ◽  
Continuous Flow ◽  
Flow System ◽  
Low Cost ◽  
Immobilized Enzymes ◽  
Glucose Determination ◽  
Nylon Tube

Abstract We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine determination of glucose at very low cost. Precision is good, and results correlate well with those by the method involving glucose oxidase in solution. More than 3000 tests may be carried out with one reactor. The immobilized enzymes are stable for several months at 4 degrees C when not in use.

Horseradish peroxidase biosensor based on layer-by-layer technique for the determination of phenolic compounds

2006 ◽  
Vol 114 (2) ◽  
pp. 774-780 ◽  
Author(s):  
Shaoming Yang ◽  
Yangmei Li ◽  
Xiuming Jiang ◽  
Zhichun Chen ◽  
Xianfu Lin
Keyword(s):  
Phenolic Compounds ◽  
Horseradish Peroxidase ◽  
Layer By Layer ◽  
Layer Technique ◽  
Layer By Layer Technique
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