Evaluation of acoustic properties of the live human smooth-muscle cell using scanning acoustic microscopy

1998 ◽  
Vol 24 (9) ◽  
pp. 1397-1405 ◽  
Author(s):  
Aki Kinoshita ◽  
Shoichi Senda ◽  
Katsufumi Mizushige ◽  
Hisashi Masugata ◽  
Seiji Sakamoto ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Acoustic Microscopy ◽  
Acoustic Properties ◽  
Scanning Acoustic Microscopy ◽  
Human Smooth Muscle Cell

Inhibition of Human Smooth Muscle Cell Proliferation by Gamigeonsim-Tang through the transcriptional Regulation of Cell Cycle-Controlling Genes

2000 ◽  
Vol 28 (01) ◽  
pp. 57-67 ◽  
Author(s):  
Seong-Gyu Ko ◽  
Kyung-Sup Lee ◽  
Ki-Ho Cho ◽  
Young-Suk Kim ◽  
Hyung-Sup Bae ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Cycle Progression ◽  
Cellular Proliferation ◽  
Flow Cytometric Analysis ◽  
Pcr Analysis ◽  
Cell Cycle Regulators ◽  
Human Smooth Muscle Cell

The effects of Gamigeonsim-tang (GGT) on cellular proliferation and expression of cell cycle-related genes were investigated in human smooth muscle cell HISM. HISM cells were treated with an aqueous extract of GGT. Cellular proliferation was investigated by an immunocytometric analysis of PCNA expression and a flow cytometric analysis of the cell cycle progression. Reduced expression of PCNA and a significant accumulation of G1phase cells were observed following treatment, indicating that GGT inhibits cellular proliferation of human smooth muscle cells. To explore whether GGT affects the transcription of cell cycle-regulating genes, we evaluated mRNA expression of p53, p21Waf1, PCNA, Cyclin D1, Cdc2, Histone H3, c-Myc, and c-Fos using a quantitative RT-PCR analysis. While increased expressions of two negative cell cycle regulators, p53 and p21Waf1were found, reduced expressions of cell cycle stimulators, PCNA, c-Fos, and c-Myc, were identified following treatment. Taken together, our study demonstrates that GGT inhibits cellular proliferation of human smooth muscle cell through the up- and down-regulation of growth-inhibiting and growth-promoting genes, respectively.

Characterization of the Human Smooth Muscle Cell Secretome for Regenerative Medicine

2012 ◽  
Vol 18 (10) ◽  
pp. 797-816 ◽  
Author(s):  
Dominic M. Justewicz ◽  
Jacob E. Shokes ◽  
Bethany Reavis ◽  
Sarah A. Boyd ◽  
Teresa B. Burnette ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Regenerative Medicine ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Human Smooth Muscle Cell

MMP-3 (5A/6A) Polymorphism Does Not Influence Human Smooth Muscle Cell Invasion

2012 ◽  
Vol 175 (2) ◽  
pp. 343-349 ◽  
Author(s):  
Azhar Maqbool ◽  
Anita Keswani ◽  
Stacey Galloway ◽  
David J. O’Regan ◽  
Stephen G. Ball ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Invasion ◽  
Human Smooth Muscle Cell

scholarly journals Therapeutic Angiogenesis Using Tissue Engineered Human Smooth Muscle Cell Sheets

2008 ◽  
Vol 28 (4) ◽  
pp. 637-643 ◽  
Author(s):  
Kyoko Hobo ◽  
Tatsuya Shimizu ◽  
Hidekazu Sekine ◽  
Toshiharu Shin’oka ◽  
Teruo Okano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Therapeutic Angiogenesis ◽  
Cell Sheets ◽  
Human Smooth Muscle Cell
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