Genotype-dependent in vitro response of date palm (Phoenix dactylifera L.) cultivars to silver nitrate

One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, explants did not have swelling, callus or root formation. The treatment containing 150 mg/L 2,4-D in the presence of 1.5 mg/L 2iP initiated explant swelling, and treatments with either 100 mg/L or 150 mg/L 2,4-D, combined with 3.0 mg/L 2iP, were also efficient in stimulating in vitro swelling of inflorescence buds. Rhizogenesis was induced at the highest concentrations of 2,4-D (100 and 150 mg/L), combined with 4.5 mg/L 2iP, and was visually more evident in the treatment containing 150 mg/L 2,4-D + 4.5 mg/L 2iP. These results suggest that even higher concentrations of these two reagents might be efficient in the micropropagation of new existing date palm genotypes in the Submedium São Francisco River Valley.

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In vitro propagation of the endangered date palm (Phoenix dactylifera L.) cv. Ghazal: 2- Improvement of germination and development of embryos by silver nitrate and silver thiosulfate

IOSR Journal of Biotechnology and Biochemistry ◽

10.9790/264x-03046674 ◽

2017 ◽

Vol 03 (04) ◽

pp. 66-74

Author(s):

Mohamed Ismail Diab

Keyword(s):

Silver Nitrate ◽

In Vitro Propagation ◽

Date Palm ◽

Phoenix Dactylifera ◽

Silver Thiosulfate ◽

Phoenix Dactylifera L

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2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

10.21203/rs.3.rs-777517/v1 ◽

2021 ◽

Author(s):

Emna Baklouti ◽

Thierry Beulé ◽

Amèni Nasri ◽

Amal Ben Romdhane ◽

Riadh Drira ◽

...

Keyword(s):

Date Palm ◽

Specific Activity ◽

Phoenix Dactylifera ◽

Proline Content ◽

Epigenetic Changes ◽

Negative Effects ◽

Somaclonal Variations ◽

Phoenix Dactylifera L ◽

Free Media

Abstract The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L− 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g− 1 to 2973 nmol g− 1 FW when 40 mg L− 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA); likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8–18.93%.

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In vitro and in situ inhibition of some food-borne pathogens by essential oils from date palm (Phoenix dactylifera L.) spathe

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2019.03.018 ◽

2019 ◽

Vol 299 ◽

pp. 64-70 ◽

Cited By ~ 6

Author(s):

Najeeb S. Al-zoreky ◽

Abdulla Y. Al-Taher

Keyword(s):

Essential Oils ◽

Date Palm ◽

Phoenix Dactylifera ◽

Food Borne Pathogens ◽

Food Borne ◽

Phoenix Dactylifera L

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In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v23i2.17522 ◽

2014 ◽

Vol 23 (2) ◽

pp. 211-219

Author(s):

Md. Abul Kalam Azad ◽

Hasnatul Arefin ◽

Md. Amzad Hossain

Keyword(s):

Date Palm ◽

Callus Formation ◽

Shoot Proliferation ◽

Phoenix Dactylifera ◽

Shoot Induction ◽

In Vitro Morphogenesis ◽

Shoot Initiation ◽

Phoenix Dactylifera L ◽

Young Leaves

After inoculation of young leaves of date palm offshoot required about six months to complete the morphogenesis process. Fourteen weeks were required for embryogenic callus formation under continuous dark condition and nine weeks for shoot initiation (under 16/8 h light/dark). The highest number of explants (80%) produced callus in modified MS containing 5 mg/l 2,4-D + 2 mg/l 2ip. Sixty per cent of explants produced callus in the modified medium containing 5 mg/l 2,4-D + 5 mg/l NAA. while only 50 per cent of the explants formed callus in the same medium when supplemented with only 5 mg/l 2,4-D. The induced calli were transferred to modified MS for shoot proliferation. A combination of two cytokines showed better performance than single ones in shoot induction. The highest percentage (70) of shoot developed in modified MS containing 2 mg/l BAP + 1 mg/l Kn. Forty per cent shoot induction was found in the same medium supplemented with 2 mg/l of BAP. Thirty per cent shoot formed in MS containing 1 mg/l of Kn. The shoots were subcultured at three- four week intervals throughout culture duration. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17522 Plant Tissue Cult. & Biotech. 23(2): 211-219, 2013 (December)

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