A reverse nuclear factor-κB element in the rat type II nitric oxide synthase promoter mediates the induction by interleukin-1β and interferon-γ in rat aortic smooth muscle cells

2000 ◽  
Vol 34 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Xingwu Teng ◽  
Hanfang Zhang ◽  
Connie Snead ◽  
John D Catravas
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Synthase ◽  
Smooth Muscle Cells ◽  
Nuclear Factor Κb ◽  
Interferon Γ ◽  
Nuclear Factor ◽  
Type Ii ◽  
Aortic Smooth Muscle ◽  
Oxide Synthase

Induction and cDNA sequence of inducible nitric oxide synthase from canine aortic smooth muscle cells

1998 ◽  
Vol 275 (4) ◽  
pp. H1122-H1129 ◽  
Author(s):  
Xiaofang Wang ◽  
Christopher G. A. McGregor ◽  
Virginia M. Miller
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Synthase ◽  
Smooth Muscle Cells ◽  
Allograft Rejection ◽  
Muscle Cells ◽  
Type Ii ◽  
Rt Pcr ◽  
Aortic Smooth Muscle ◽  
Oxide Synthase

An inducible isoform of nitric oxide synthase (type II, iNOS) is expressed in cardiac and vascular smooth muscle in response to inflammatory cytokines. The dog is an important large animal used for cardiovascular research including effects of exercise, heart failure, and allograft rejection. However, molecular probes for iNOS developed in other mammals have not been reliable for the study of iNOS induction in canine vascular smooth muscle. Experiments were designed to develop a molecular probe for canine iNOS. Smooth muscle cells were isolated from canine aortas. The cells ( passages 3–10) were incubated for 1, 3, 6, 12, 24, 48, or 72 h in the absence and presence of Escherichia coli lipopolysaccharide (LPS) to induce iNOS. Total RNA was isolated from the cells using standard techniques. RT-PCR with primers against conserved regions of all known iNOS enzyme was used to clone the iNOS cDNA. RT-PCR showed a single band only from cells treated with LPS. Cloned cDNA from cultured canine aortic smooth muscle cells has 84% homology to human, 81% to rat, and 81% to mouse iNOS gene. Identification of the cDNA for canine iNOS will be useful in the study of differential, transcriptional regulation of inducible (type II) compared with constitutive endothelial (type III) NOS in canine studies of allograft rejection and cardiovascular disease.

scholarly journals Antioxidants differentially affect nuclear factor κB-mediated nitric oxide synthase expression in vascular smooth muscle cells

FEBS Letters ◽  
1996 ◽  
Vol 380 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Markus Hecker ◽  
Christiane Preiβ ◽  
Valérie B. Schini-Kerth ◽  
Rudi Busse
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Synthase ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Oxide Synthase

Hypertonicity Inhibits Lipopolysaccharide-Induced Nitric Oxide Synthase Expression in Smooth Muscle Cells by Inhibiting Nuclear Factor κB

2003 ◽  
Vol 63 (6) ◽  
pp. 1238-1247 ◽  
Author(s):  
Sandeep C. Pingle ◽  
Joseph F. Sanchez ◽  
Daniel M. Hallam ◽  
Andrea L. Williamson ◽  
Sanjay B. Maggirwar ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Synthase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Oxide Synthase
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