Suppression of the morphine-induced rewarding effect and G-protein activation in the lower midbrain following nerve injury in the mouse: involvement of G-protein-coupled receptor kinase 2

Neuroscience ◽  
2003 ◽  
Vol 116 (1) ◽  
pp. 89-97 ◽  
Author(s):  
S. Ozaki ◽  
M. Narita ◽  
M. Narita ◽  
M. Iino ◽  
K. Miyoshi ◽  
...  
Keyword(s):  
G Protein ◽  
Nerve Injury ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Rewarding Effect ◽  
Protein Activation ◽  
G Protein Activation ◽  
G Protein Coupled

scholarly journals C-terminal threonines and serines play distinct roles in the desensitization of rhodopsin, a G protein-coupled receptor

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Anthony W Azevedo ◽  
Thuy Doan ◽  
Hormoz Moaven ◽  
Iza Sokal ◽  
Faiza Baameur ◽  
...  
Keyword(s):  
G Protein ◽  
Single Photon ◽  
Low Noise ◽  
Tight Control ◽  
G Protein Coupled Receptor ◽  
Rod Photoreceptors ◽  
Protein Activation ◽  
G Protein Activation ◽  
G Protein Coupled

Rod photoreceptors generate measurable responses to single-photon activation of individual molecules of the G protein-coupled receptor (GPCR), rhodopsin. Timely rhodopsin desensitization depends on phosphorylation and arrestin binding, which quenches G protein activation. Rhodopsin phosphorylation has been measured biochemically at C-terminal serine residues, suggesting that these residues are critical for producing fast, low-noise responses. The role of native threonine residues is unclear. We compared single-photon responses from rhodopsin lacking native serine or threonine phosphorylation sites. Contrary to expectation, serine-only rhodopsin generated prolonged step-like single-photon responses that terminated abruptly and randomly, whereas threonine-only rhodopsin generated responses that were only modestly slower than normal. We show that the step-like responses of serine-only rhodopsin reflect slow and stochastic arrestin binding. Thus, threonine sites play a privileged role in promoting timely arrestin binding and rhodopsin desensitization. Similar coordination of phosphorylation and arrestin binding may more generally permit tight control of the duration of GPCR activity.

A concept for G protein activation by G protein-coupled receptor dimers: the transducin/rhodopsin interface

2004 ◽  
Vol 3 (6) ◽  
pp. 628 ◽  
Author(s):  
Slawomir Filipek ◽  
Krystiana A. Krzysko ◽  
Dimitrios Fotiadis ◽  
Yan Liang ◽  
David A. Saperstein ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptor ◽  
Protein Activation ◽  
G Protein Activation ◽  
Receptor Dimers ◽  
G Protein Coupled

Micropatterned immobilization of a G protein–coupled receptor and direct detection of G protein activation

10.1038/15090 ◽  
1999 ◽  
Vol 17 (11) ◽  
pp. 1105-1108 ◽  
Author(s):  
Christoph Bieri ◽  
Oliver P. Ernst ◽  
Stephan Heyse ◽  
Klaus Peter Hofmann ◽  
Horst Vogel
Keyword(s):  
G Protein ◽  
Direct Detection ◽  
G Protein Coupled Receptor ◽  
Protein Activation ◽  
G Protein Activation ◽  
G Protein Coupled
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