Detection of different types of receptors for deoxyribonucleic acid in competent Bacillus subtilis

1981 ◽  
Vol 136 (6) ◽  
pp. 524-532
Author(s):  
Paloma López ◽  
M. Tekesa Pérez Ureña ◽  
Ernesto García ◽  
M. Teresa Alda ◽  
Manolo Espinosa
Keyword(s):  
Bacillus Subtilis ◽  
Deoxyribonucleic Acid ◽  
Different Types

The initial synthesis of haemoglobin in de-embryonated chick blastoderms.

Development ◽  
1964 ◽  
Vol 12 (4) ◽  
pp. 609-619
Author(s):  
Anna Hell
Keyword(s):  
Protein Synthesis ◽  
Deoxyribonucleic Acid ◽  
Primitive Streak ◽  
Specific Protein ◽  
In Vitro System ◽  
Embryonic Tissues ◽  
Different Types ◽  
Excellent Tool ◽  
Made In

Enormous progress has been made in the last few years towards the elucidation of the mechanism of protein synthesis, and great interest is centred on the steps leading to cellular differentiation and specific protein synthesis. We know that genetic information is passed on from one generation of cells to the next by deoxyribonucleic acid (DNA), and that this material directs all protein synthesis by the intermediary of the different types of ribonucleic acid (RNA). A simple in vitro system described by O'Brien (1959) seemed to offer an excellent tool for the study of the differentiation of the blood islands, and the initial formation of a well-known protein, haemoglobin (Hb), in chick embryonic tissues. After de-embryonation, chick blastoderms, from the stage of primitive streak onwards, can be cultured in vitro on a saline agar medium supplemented with glucose.

scholarly journals Thymineless Death in Bacillus subtilis: Correlation Between Cell Lysis and Deoxyribonucleic Acid Breakdown

1974 ◽  
Vol 119 (1) ◽  
pp. 36-43 ◽  
Author(s):  
E. Ephrati-Elizur ◽  
D. Yosuv ◽  
E. Shmueli ◽  
A. Horowitz
Keyword(s):  
Bacillus Subtilis ◽  
Deoxyribonucleic Acid ◽  
Cell Lysis ◽  
Thymineless Death

scholarly journals Estimation of permeability to bacteriophages of intestinal mucosa of chickens with eimeriosis

2019 ◽  
Vol 49 (2) ◽  
pp. 57-63
Author(s):  
N. V. Davydova ◽  
V. Yu. Koptev ◽  
Yu. N. Kozlova ◽  
L. I. Sulimova ◽  
V. N. Afonyushkin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Protective Effect ◽  
Intestinal Mucosa ◽  
Clinical Picture ◽  
Biological Material ◽  
Probiotic Strain ◽  
Field Isolates ◽  
Different Types ◽  
Inflammatory Processes

In the course of the study permeability of intestinal mucosa of chickens suffering from eimeriosis while treating them with various veterinary drugs, including probiotics, was evaluated. The simulation of a typical clinical picture of eimeriosis was carried out by oral administration of suspension with coccidial oocysts (1.6 × 105/head) using a probe. To create different forms and different intensity of inflammatory processes, chickens that received various anticoccidial preparations and probiotic strain Bacillus subtilis were infected with eimeria. According to the data from an autopsy, it was found that the use of a spore probiotic based on Bacillus subtilis and anticoccidial drugs containing robenidine hydrochloride and salinomycin had a positive protective effect when treating chickens from eimeriosis. A similar picture was observed when assessing permeability of intestinal mucosa as affected by bacteriophage, whereby permeability decreased with the use of probiotics and the above-mentioned active agents. In general, the decrease in productivity was significant in all groups. However, the effect of spore-based probiotics was quite pronounced against the background of eimeria polyresistance. In the situation where anticoccidial drugs are less effective, the use of a spore-based probiotic can have a noticeable protective effect. The effect of all anticoccidial drugs under study on the concentration of oocysts and the state of the mucosa was insignificant, which indicated polyresistance of different types of eimeria isolated from biological material to these drugs. The analysis of the intestinal mucosa integrity, based on the study of mucosa permeability to bacteriophages and a Johnson and Reid scoring procedure showed that a spore probiotic based on B. subtilis and anticoccidial drugs containing robenidine and salinomycin had the best protective effect against eimeriosis caused by field isolates of eimeria. When treating chickens suffering from eimeriosis caused by polyresistant forms of E. acervulina and E. tenella, it is advisable to use probiotics alongside with drugs based on robenidine and salinomycin.

scholarly journals Fractionation of native and denatured transforming deoxyribonucleic acid from Bacillus subtilis

1968 ◽  
Vol 107 (3) ◽  
pp. 387-393 ◽  
Author(s):  
S R Ayad ◽  
G R Barker ◽  
Josephine Weigold
Keyword(s):  
Bacillus Subtilis ◽  
Deoxyribonucleic Acid ◽  
Residual Activity ◽  
Specific Component ◽  
Partial Separation ◽  
Partial Denaturation ◽  
Transforming Activity

1. Native DNA from Bacillus subtilis was fractionated by stepwise elution from methylated albumin, the transforming activity being confined to two out of four fractions. Partial separation of DNA active in transformation for the arginine marker from that showing activity for the histidine and tryptophan markers was achieved. 2. Partial denaturation of DNA at 90° and 93·5° resulted in the preferential destruction of transforming activity for the histidine and tryptophan markers. 3. Denaturation of DNA at 100° followed by chromatography on methylated albumin yielded five fractions, two of which exhibited residual activity. Redenaturation at 100° resulted in the interconversion of four out of the five fractions. Redenaturation of fractions labelled with 15N and 2H suggested the presence of a specific component that did not readily take part in the interconversions.

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