Truncated chlorophyll antenna size of the photosystems?a practical method to improve microalgal productivity and hydrogen production in mass culture

2002 ◽  
Vol 27 (11-12) ◽  
pp. 1257-1264 ◽  
Author(s):  
J POLLE
Keyword(s):  
Hydrogen Production ◽  
Mass Culture ◽  
Practical Method ◽  
Antenna Size ◽  
Chlorophyll Antenna Size

scholarly journals Modeling the competition between antenna size mutant and wild type microalgae in outdoor mass culture

2016 ◽  
Vol 240 ◽  
pp. 1-13 ◽  
Author(s):  
Tim de Mooij ◽  
Kira Schediwy ◽  
René H. Wijffels ◽  
Marcel Janssen
Keyword(s):  
Mass Culture ◽  
Wild Type ◽  
Antenna Size ◽  
Outdoor Mass Culture

scholarly journals Modulation of the light-harvesting chlorophyll antenna size in Chlamydomonas reinhardtii by TLA1 gene over-expression and RNA interference

2012 ◽  
Vol 367 (1608) ◽  
pp. 3430-3443 ◽  
Author(s):  
Mautusi Mitra ◽  
Henning Kirst ◽  
David Dewez ◽  
Anastasios Melis
Keyword(s):  
Gene Expression ◽  
Chlamydomonas Reinhardtii ◽  
Light Harvesting ◽  
Thylakoid Membranes ◽  
Chloroplast Envelope ◽  
Cell Fractionation ◽  
Reaction Centre ◽  
Antenna Size ◽  
Over Expression ◽  
Chlorophyll Antenna Size

Truncated light-harvesting antenna 1 ( TLA1 ) is a nuclear gene proposed to regulate the chlorophyll (Chl) antenna size in Chlamydomonas reinhardtii . The Chl antenna size of the photosystems and the chloroplast ultrastructure were manipulated upon TLA1 gene over-expression and RNAi downregulation. The TLA1 over-expressing lines possessed a larger chlorophyll antenna size for both photosystems and contained greater levels of Chl b per cell relative to the wild type. Conversely, TLA1 RNAi transformants had a smaller Chl antenna size for both photosystems and lower levels of Chl b per cell. Western blot analyses of the TLA1 over-expressing and RNAi transformants showed that modulation of TLA1 gene expression was paralleled by modulation in the expression of light-harvesting protein, reaction centre D1 and D2, and VIPP1 genes. Transmission electron microscopy showed that modulation of TLA1 gene expression impacts the organization of thylakoid membranes in the chloroplast. Over-expressing lines showed well-defined grana, whereas RNAi transformants possessed loosely held together and more stroma-exposed thylakoids. Cell fractionation suggested localization of the TLA1 protein in the inner chloroplast envelope and potentially in association with nascent thylakoid membranes, indicating a role in Chl antenna assembly and thylakoid membrane biogenesis. The results provide a mechanistic understanding of the Chl antenna size regulation by the TLA1 gene.

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