Affinity selection of cDNA libraries by λ phage surface display

Gene ◽  
2000 ◽  
Vol 256 (1-2) ◽  
pp. 229-236 ◽  
Author(s):  
Mikio Niwa ◽  
Hiroko Maruyama ◽  
Takashi Fujimoto ◽  
Kazuhiro Dohi ◽  
Ichi N Maruyama
Keyword(s):  
Surface Display ◽  
Cdna Libraries ◽  
Affinity Selection ◽  
Selection Of

scholarly journals Mammalian cell surface display for monoclonal antibody-based FACS selection of viral envelope proteins

mAbs ◽  
2017 ◽  
Vol 9 (7) ◽  
pp. 1052-1064 ◽  
Author(s):  
Tim-Henrik Bruun ◽  
Veronika Grassmann ◽  
Benjamin Zimmer ◽  
Benedikt Asbach ◽  
David Peterhoff ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Surface ◽  
Mammalian Cell ◽  
Surface Display ◽  
Cell Surface Display ◽  
Envelope Proteins ◽  
Viral Envelope ◽  
Selection Of ◽  
Viral Envelope Proteins

scholarly journals Size-selection of cDNA libraries for the cloning of cDNAs after suppression subtractive hybridization

BioTechniques ◽  
2003 ◽  
Vol 35 (1) ◽  
pp. 72-78 ◽  
Author(s):  
V. Lévesque ◽  
T. Fayad ◽  
K. Ndiaye ◽  
M. Nahé Diouf ◽  
J.G. Lussier
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Subtractive Hybridization ◽  
Cdna Libraries ◽  
Size Selection ◽  
Selection Of

scholarly journals MATURATION OF THE IMMUNE RESPONSE IN VITRO

1972 ◽  
Vol 136 (2) ◽  
pp. 353-368 ◽  
Author(s):  
Alberto J. L. Macario ◽  
Everly Conway de Macario ◽  
Claudio Franceschi ◽  
Franco Celada
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Lymph Node ◽  
Association Constant ◽  
Secondary Response ◽  
Antibody Specificity ◽  
Affinity Selection ◽  
Immune Response In Vitro ◽  
Selection Of

We have cultivated lymph node microfragments from ß-D-galactosidase (Escherichia coli) primed rabbits and have measured their secondary response directed towards the whole molecule (precipitating antibodies) and to a single determinant (activating antibodies) of the antigen. By decreasing the size of the fragments to 105 cells, we began to observe heterogeneity among identical cultures in terms of positivity of response, antibody specificity, and titers. The affinity of "early" activating antibodies was inversely proportional to the dose of challenge. While no maturation was seen in low and excessive challenge, in all cultures receiving intermediate doses the association constant was raised several orders of magnitude within periods of 20 days. The relevance of these data to the mechanism of affinity selection of antigen-sensitive cells is discussed.

scholarly journals Affinity selection of epitope-based vaccines using a bacteriophage virus-like particle platform

2015 ◽  
Vol 11 ◽  
pp. 76-82 ◽  
Author(s):  
John P O’Rourke ◽  
David S Peabody ◽  
Bryce Chackerian
Keyword(s):  
Affinity Selection ◽  
Virus Like Particle ◽  
Selection Of

Phage presentation and affinity selection of a deletion mutant of human lnterleukin-3

1997 ◽  
Vol 67 (3) ◽  
pp. 199-214 ◽  
Author(s):  
Sean Merlin ◽  
Edwin Rowold ◽  
Ann Abegg ◽  
Cathleen Berglund ◽  
Jon Klover ◽  
...  
Keyword(s):  
Deletion Mutant ◽  
Affinity Selection ◽  
Selection Of

11 Serological antigen selection of phage displayed colorectal tumour cDNA libraries

1998 ◽  
Vol 26 (1) ◽  
pp. S5-S5 ◽  
Author(s):  
Simon. E. Hufton ◽  
Peter Moerkerk ◽  
Adriaan de Bruine ◽  
Jan-Willem Arends ◽  
Hennie. R. Hoogenboom
Keyword(s):  
Cdna Libraries ◽  
Colorectal Tumour ◽  
Selection Of ◽  
Antigen Selection

scholarly journals Affinity selection of a camelized V(H) domain antibody inhibitor of hepatitis C virus NS3 protease

1997 ◽  
Vol 10 (5) ◽  
pp. 607-614 ◽  
Author(s):  
F. Martin ◽  
C. Volpari ◽  
C. Steinkuhler ◽  
N. Dimasi ◽  
M. Brunetti ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Affinity Selection ◽  
Domain Antibody ◽  
Ns3 Protease ◽  
Selection Of

Efficient one-cycle affinity selection of binding proteins or peptides specific for a small-molecule using a T7 phage display pool

2008 ◽  
Vol 16 (22) ◽  
pp. 9837-9846 ◽  
Author(s):  
Yoichi Takakusagi ◽  
Kouji Kuramochi ◽  
Manami Takagi ◽  
Tomoe Kusayanagi ◽  
Daisuke Manita ◽  
...  
Keyword(s):  
Phage Display ◽  
Small Molecule ◽  
Binding Proteins ◽  
Affinity Selection ◽  
T7 Phage Display ◽  
T7 Phage ◽  
Selection Of
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