Structure analysis of a class II transposon encoding the mercury resistance of the Gram-positive bacterium Bacillus megaterium MB1, a strain isolated from Minamata Bay, Japan

Gene ◽  
1999 ◽  
Vol 234 (2) ◽  
pp. 361-369 ◽  
Author(s):  
Chieh-Chen Huang ◽  
Masaru Narita ◽  
Takeshi Yamagata ◽  
Yukihiro Itoh ◽  
Ginro Endo
Keyword(s):  
Structure Analysis ◽  
Bacillus Megaterium ◽  
Class Ii ◽  
Mercury Resistance ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Minamata Bay ◽  
Bacterium Bacillus

scholarly journals Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Naomi Shimokawa-Chiba ◽  
Claudia Müller ◽  
Keigo Fujiwara ◽  
Bertrand Beckert ◽  
Koreaki Ito ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stop Codon ◽  
Release Factor ◽  
Positive Bacterium ◽  
Gram Positive ◽  
E Coli ◽  
Cryo Electron Microscopy ◽  
Dead End ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

AbstractRescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop-codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named BrfA. Genetic analysis shows that B. subtilis requires the function of either trans-translation or BrfA for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-electron microscopy (cryo-EM) characterization demonstrates that BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although BrfA is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.

scholarly journals ResQ, a release factor-dependent ribosome rescue factor in the Gram-positive bacterium Bacillus subtilis

2019 ◽  
Author(s):  
Naomi Shimokawa-Chiba ◽  
Claudia Müller ◽  
Keigo Fujiwara ◽  
Bertrand Beckert ◽  
Koreaki Ito ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stop Codon ◽  
Release Factor ◽  
Active Conformation ◽  
Positive Bacterium ◽  
Gram Positive ◽  
E Coli ◽  
Dead End ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

SummaryRescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named ResQ. Genetic analysis shows that B. subtilis requires the function of either trans-translation or ResQ for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-EM characterization demonstrates that ResQ binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although ResQ is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.

scholarly journals Identification of A Novel Arsenic Resistance Transposon Nested in A Mercury Resistance Transposon of Bacillus sp. MB24

2019 ◽  
Vol 7 (11) ◽  
pp. 566 ◽  
Author(s):  
Mei-Fang Chien ◽  
Ying-Ning Ho ◽  
Hui-Erh Yang ◽  
Masaru Narita ◽  
Keisuke Miyauchi ◽  
...  
Keyword(s):  
Gene Clusters ◽  
Whole Genome Sequence ◽  
Mercury Resistance ◽  
Bacillus Sp ◽  
Arsenic Resistance ◽  
First Case ◽  
Arsenite Resistance ◽  
Ars Operon ◽  
Minamata Bay ◽  
Bacterium Bacillus

A novel TnMERI1-like transposon designated as TnMARS1 was identified from mercury resistant Bacilli isolated from Minamata Bay sediment. Two adjacent ars operon-like gene clusters, ars1 and ars2, flanked by a pair of 78-bp inverted repeat sequences, which resulted in a 13.8-kbp transposon-like fragment, were found to be sandwiched between two transposable genes of the TnMERI1-like transposon of a mercury resistant bacterium, Bacillus sp. MB24. The presence of a single transcription start site in each cluster determined by 5′-RACE suggested that both are operons. Quantitative real time RT-PCR showed that the transcription of the arsR genes contained in each operon was induced by arsenite, while arsR2 responded to arsenite more sensitively and strikingly than arsR1 did. Further, arsenic resistance complementary experiments showed that the ars2 operon conferred arsenate and arsenite resistance to an arsB-knocked out Bacillus host, while the ars1 operon only raised arsenite resistance slightly. This transposon nested in TnMARS1 was designated as TnARS1. Multi-gene cluster blast against bacteria and Bacilli whole genome sequence databases suggested that TnMARS1 is the first case of a TnMERI1-like transposon combined with an arsenic resistance transposon. The findings of this study suggested that TnMERI1-like transposons could recruit other mobile elements into its genetic structure, and subsequently cause horizontal dissemination of both mercury and arsenic resistances among Bacilli in Minamata Bay.

scholarly journals Aerobic degradation of fenvalerate by a Gram-positive bacterium, Bacillus flexus strain XJU-4

3 Biotech ◽  
2017 ◽  
Vol 7 (5) ◽  
Author(s):  
Sikandar I. Mulla ◽  
Fuad Ameen ◽  
Preeti N. Tallur ◽  
Ram Naresh Bharagava ◽  
Manjunatha Bangeppagari ◽  
...  
Keyword(s):  
Aerobic Degradation ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Bacillus Flexus ◽  
Bacterium Bacillus

scholarly journals The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis

2000 ◽  
Vol 351 (3) ◽  
pp. 839-839
Author(s):  
K. STEPHENSON ◽  
C. L. JENSEN ◽  
S. T. JØRGENSEN ◽  
J. H. LAKEY ◽  
C. R. HARWOOD
Keyword(s):  
Bacillus Subtilis ◽  
Secretory Protein ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Protein Charge ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Late Stages

Class II broad-spectrum mercury resistance transposons in Gram-positive bacteria from natural environments

2001 ◽  
Vol 152 (5) ◽  
pp. 503-514 ◽  
Author(s):  
Elena Bogdanova ◽  
Leonid Minakhin ◽  
Irina Bass ◽  
Alexander Volodin ◽  
Jon L. Hobman ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Class Ii ◽  
Mercury Resistance ◽  
Natural Environments ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Phylogenetic analysis of microorganisms from permafrost

2016 ◽  
pp. 92-95
Author(s):  
G. M. Edidin ◽  
A. V. Brouchkov ◽  
S. G. Ignatov
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Isolation ◽  
Microbiological Analysis ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Mammoth Mountain ◽  
Bacillus Genus ◽  
The Republic ◽  
Bacterium Bacillus

From the permafrost of Mammoth Mountain in the Republic of Saha (Yakutia) samples were obtained for subsequent microbiological analysis. We were isolated Gram-positive bacterium Bacillus genus. Conducted DNA isolation and sequencing of the isolated bacteria. Phylogenetic analysis of the microorganisms from the permafrost.

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