In vitro nephrotoxicity testing: Polarized approach of proximal tubular epithelium

1998 ◽  
Vol 95 ◽  
pp. 167
Author(s):  
I. Genestie ◽  
V. Keravec ◽  
J.P. Morin ◽  
J.P. Fillastre
Keyword(s):  
Tubular Epithelium ◽  
Proximal Tubular ◽  
Proximal Tubular Epithelium

scholarly journals Renal proximal tubular epithelium from patients with nephropathic cystinosis: Immortalized cell lines as in vitro model systems

1995 ◽  
Vol 48 (2) ◽  
pp. 536-543 ◽  
Author(s):  
Lorraine C. Racusen ◽  
◽  
Patricia D. Wilson ◽  
Patricia A. Hartz ◽  
Barbara A. Fivush ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Model Systems ◽  
Nephropathic Cystinosis ◽  
Tubular Epithelium ◽  
Proximal Tubular ◽  
Vitro Model ◽  
Renal Proximal Tubular ◽  
Immortalized Cell ◽  
Proximal Tubular Epithelium

scholarly journals Molecular Mechanisms of Receptor-Mediated Endocytosis in the Renal Proximal Tubular Epithelium

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Akihiko Saito ◽  
Hiroyoshi Sato ◽  
Noriaki Iino ◽  
Tetsuro Takeda
Keyword(s):  
Molecular Mechanisms ◽  
Adaptor Proteins ◽  
Risk Marker ◽  
Tubular Epithelium ◽  
Coated Pits ◽  
Receptor Mediated Endocytosis ◽  
Other Substances ◽  
Proximal Tubular ◽  
Renal Proximal Tubular ◽  
Proximal Tubular Epithelium

Receptor-mediated endocytosis is a pivotal function of renal proximal tubule epithelial cells (PTECs) to reabsorb and metabolize substantial amounts of proteins and other substances in glomerular filtrates. The function accounts for the conservation of nutrients, including carrier-bound vitamins and trace elements, filtered by glomeruli. Impairment of the process results in a loss of such substances and development of proteinuria, an important clinical sign of kidney disease and a risk marker for cardiovascular disease. Megalin is a multiligand endocytic receptor expressed at clathrin-coated pits of PTEC, playing a central role in the process. Megalin cooperates with various membrane molecules and interacts with many intracellular adaptor proteins for endocytic trafficking. Megalin is also involved in signaling pathways in the cells. Megalin-mediated endocytic overload leads to damage of PTEC. Further studies are needed to elucidate the mechanism of megalin-mediated endocytosis and develop strategies for preventing the damage of PTEC.

scholarly journals Transcellular Diffusion of Non-Electrolytes across the Renal Tubular Epithelium

1962 ◽  
Vol 45 (4) ◽  
pp. 643-649 ◽  
Author(s):  
José Carlos Peña ◽  
Richard L. Malvin
Keyword(s):  
Tubular Epithelium ◽  
Permeability Characteristics ◽  
Molecular Weights ◽  
Renal Tubular Epithelium ◽  
Significant Movement ◽  
Proximal Tubular ◽  
Renal Tubular ◽  
Proximal Tubular Epithelium ◽  
Stop Flow ◽  
Transcellular Diffusion

The stop flow technique was used to investigate the permeability characteristics of the dog nephron to various C14-labeled non-electrolytes. 12 minutes after clamping the ureter, creatinine, PAH, and C14 compound were injected intravenously. 2 minutes later, urine samples were collected. Urea and glycerol were able to enter the tubular urine along the entire nephron at rates which were commensurate with their molecular weights. No significant movement of larger molecules (D-arabinose, D-glucose, and mannitol) could be detected. However, after administration of twenty units of pitressin, D-arabinose was able to diffuse across the distal and proximal tubular epithelium.

scholarly journals Netrin-1 overexpression in kidney proximal tubular epithelium ameliorates cisplatin nephrotoxicity

2011 ◽  
Vol 91 (12) ◽  
pp. 1717-1726 ◽  
Author(s):  
Amala Rajasundari ◽  
Laurent Pays ◽  
Patrick Mehlen ◽  
Ganesan Ramesh
Keyword(s):  
Tubular Epithelium ◽  
Cisplatin Nephrotoxicity ◽  
Proximal Tubular ◽  
Proximal Tubular Epithelium

scholarly journals Influence of Native and Hypochlorite-Modified Low-Density Lipoprotein on Gene Expression in Human Proximal Tubular Epithelium

2004 ◽  
Vol 164 (6) ◽  
pp. 2175-2187 ◽  
Author(s):  
Stefan Porubsky ◽  
Holger Schmid ◽  
Mahnaz Bonrouhi ◽  
Matthias Kretzler ◽  
Ernst Malle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Tubular Epithelium ◽  
Proximal Tubular ◽  
Proximal Tubular Epithelium

Renal proximal tubular epithelium from patients with nephropathic cystinosis: Immortalized cell lines asin vitro model systems

1996 ◽  
Vol 10 (3) ◽  
pp. 394-394
Author(s):  
Lorraine C. Racusen ◽  
Patricia D. Wilson ◽  
Patricia A. Hartz ◽  
Barbara A. Fivush ◽  
Christopher R. Burrow ◽  
...  
Keyword(s):  
Cell Lines ◽  
Model Systems ◽  
Nephropathic Cystinosis ◽  
Tubular Epithelium ◽  
Immortalized Cell Lines ◽  
Proximal Tubular ◽  
Vitro Model ◽  
Renal Proximal Tubular ◽  
Immortalized Cell ◽  
Proximal Tubular Epithelium

Human Proximal Tubular Epithelium Actively Secretes but Does Not Retain Rosuvastatin

2008 ◽  
Vol 74 (4) ◽  
pp. 1084-1091 ◽  
Author(s):  
Anja Verhulst ◽  
Rachel Sayer ◽  
Marc E. De Broe ◽  
Patrick C. D'Haese ◽  
Colin D. A. Brown
Keyword(s):  
Tubular Epithelium ◽  
Proximal Tubular ◽  
Proximal Tubular Epithelium

Development of an Acute Model for the Study of Chloromethanediphosphonate Nephrotoxicity

1989 ◽  
Vol 17 (1_part_1) ◽  
pp. 27-32 ◽  
Author(s):  
Carl L. Alden ◽  
Ronald D. Parker ◽  
David F. Eastman
Keyword(s):  
Renal Toxicity ◽  
Intraperitoneal Administration ◽  
Tubular Epithelium ◽  
The Third ◽  
Pars Recta ◽  
Proximal Tubular ◽  
Histopathologic Changes ◽  
Time Required ◽  
Proximal Tubular Epithelium ◽  
Phosphorus Levels

Chloromethanediphosphonate (Cl2MDP), a cation chelator, is used as a therapeutic for hypercalcemia of malignancy. Cl2MDP exhibits nephrotoxic potential. Thus, a useful model has been developed to study the mechanism of injury. Intraperitoneal administration of highly exaggerated dosages, specifically 200 mg/kg b.i.d., resulted in a consistent mild to moderate extent of kidney damage after the third day of treatment in rats. Proteinuria and lowered serum phosphorus levels occur prior to onset of histopathologic changes. Injury was characterized as necrosis of proximal tubular epithelium with predilection for pars recta. Unlike many renal toxicity models, the necrosis occurs as cell lysis only after 24 to 48 hours of treatment. However, this model significantly reduces the time required to induce renal toxicity observed in routine toxicity studies from months of treatment to less than 1 week and will, thus, serve as a baseline for subsequent pathogenetic studies.

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