Partitioning behavior of amino acids in aqueous two-phase systems with recyclable volatile salts

2000 ◽  
Vol 743 (1-2) ◽  
pp. 317-325 ◽  
Author(s):  
Mos van Berlo ◽  
Marcel Ottens ◽  
Karel Ch.A.M Luyben ◽  
Luuk A.M van der Wielen
Keyword(s):  
Amino Acids ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Partitioning behavior of recombinant lipase in Escherichia coli by ionic liquid-based aqueous two-phase systems

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82571-82580 ◽  
Author(s):  
Muhammad Hakimi Hadzir ◽  
Sahar Abbasiliasi ◽  
Arbakariya B. Ariff ◽  
Siti Baidurah Yusoff ◽  
Hui Suan Ng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Fermentation Broth ◽  
Two Phase ◽  
E Coli ◽  
Recombinant Lipase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.

scholarly journals Thermodynamic studies of partitioning behavior of lysozyme and conalbumin in aqueous two-phase systems

2009 ◽  
Vol 877 (24) ◽  
pp. 2579-2584 ◽  
Author(s):  
Rita de Cássia Superbi de Sousa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luis Henrique Mendes da Silva ◽  
Maria do Carmo Hespanhol da Silva ◽  
Edwin Elard Garcia Rojas ◽  
...  
Keyword(s):  
Two Phase ◽  
Thermodynamic Studies ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Modeling the partitioning of amino acids in aqueous two phase systems

2014 ◽  
Vol 1329 ◽  
pp. 52-60 ◽  
Author(s):  
Dragana P.C. de Barros ◽  
Sara R.R. Campos ◽  
Pedro P. Madeira ◽  
Ana M. Azevedo ◽  
António M. Baptista ◽  
...  
Keyword(s):  
Amino Acids ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

2011 ◽  
Vol 138-139 ◽  
pp. 995-1001 ◽  
Author(s):  
Wei Jie Wang ◽  
Xiao Le Zhang ◽  
Chang Bo Xu ◽  
Hong Yan Cheng
Keyword(s):  
High Purity ◽  
Separation Process ◽  
Phase System ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Optimized Conditions ◽  
Potassium Sodium Tartrate ◽  
Partitioning Behavior

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

Separation of Casein Phosphopeptides (a Functional Food Material) by Aqueous Two-Phase Systems

2012 ◽  
Vol 554-556 ◽  
pp. 1511-1514
Author(s):  
Jiang Wu ◽  
Ying Kai Tong ◽  
Li Tong Ban
Keyword(s):  
Functional Food ◽  
Two Phase ◽  
Food Material ◽  
Casein Phosphopeptides ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

PEG 10000 / (NH4)2SO4 ATPS was applied to extract casein phosphopeptides. By studying on the partitioning behavior of CPPs in this system, it is concluded that the purification fold of CPPs could reach 7.09 when the system consisted of 20% PEG 10000 and 10% (NH4)2SO4.

scholarly journals Solubility of amino acids, sugars, and proteins

2005 ◽  
Vol 77 (3) ◽  
pp. 559-568 ◽  
Author(s):  
Eugénia Almeida Macedo
Keyword(s):  
Amino Acids ◽  
Mixed Solvents ◽  
Industrial Processes ◽  
Molecular Models ◽  
Two Phase ◽  
Range Interaction ◽  
Liquid Solutions ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Very High

The importance of biomolecules is well recognized nowadays, owing to their application in many industrial processes, particularly in the food, pharmaceutical, and cosmetic industries.Amino acids, carbohydrates, and proteins are the three types of biomolecules considered in this review. Solubilities of several amino acids and sugars have been measured in water and, more recently, in mixed solvents, both with or without salts. Experimental data on partition of proteins in aqueous two-phase systems (ATPSs), with polymers or salts, have also been reported in the literature. The experimental techniques used are briefly discussed. Regarding modeling, the complexity of these solutions is very high. Sugars form strong hydrogen bonds with one another and with water or other solvents like alcohols. Liquid solutions with amino acids or proteins present not only short-range interaction forces, but also long-range forces, owing to the appearance of charged species. The capabilities of different molecular models and group-contribution-based methods are shown.

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