Determination of leucine flux in vivo by gas chromatography—mass spectrometry utilizing stable isotopes for trace and internal standard

1980 ◽  
Vol 183 (4) ◽  
pp. 403-409 ◽  
Author(s):  
Morey W. Haymond ◽  
Campbell P. Howard ◽  
John M. Miles ◽  
John E. Gerich
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotopes ◽  
Internal Standard ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Determination of Pyrantel in Swine Liver by Flame Ionization Gas Chromatography and Confirmation by Gas Chromatography /Mass Spectrometry

1990 ◽  
Vol 73 (6) ◽  
pp. 883-886
Author(s):  
Susan S.C Tai ◽  
Nancy Cargile ◽  
Charlie J Barnes ◽  
Philip Kijak
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Internal Standard ◽  
Gas Chromatography Mass Spectrometry ◽  
Tissue Samples ◽  
Flame Ionization ◽  
Chromatography Mass Spectrometry ◽  
Ionization Method ◽  
Swine Liver

Abstract During an evaluation of the gas chromatography/mass spectrometry (GC/MS) confirmatory procedure of Lynch and Bartoluccl for pyrantel residues in swine tissues, we developed a GC flame Ionization method for quantltatlng pyrantel residues In extracts of swine liver. The method was subjected to trial principally In the laboratories of Biospherics, Inc., using control liver, fortified control liver, and Incurred liver tissue samples. Although the method does not meet all of the current Food and Drug Administration criteria, it compares favorably to the official determinative method. Portions of the same extract can be used for quantitation and for GC/MS confirmation, true recoveries appear to be slightly higher, and an internal standard Is not required. The precision of this method equals or exceeds that of the official determinative method.

Determination of Clenbuterol in Cattle, Sheep, and Swine Tissues by Electron Ionization Gas Chromatography/Mass Spectrometry

1994 ◽  
Vol 77 (4) ◽  
pp. 917-924 ◽  
Author(s):  
Roger T Wilson ◽  
Joseph M Groneck ◽  
Kathleen P Holland ◽  
A Carolyn Henry
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Internal Standard ◽  
Electron Ionization ◽  
Gas Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Method Performance ◽  
Chromatography Mass Spectrometry ◽  
Coefficients Of Variation

Abstract A gas chromatographic/mass spectrometric procedure is described for the quantitation and confirmation of clenbuterol residues from cattle, sheep, and swine tissues. After liquid–liquid extraction and derivatization with phosgene in an aqueous pH 10.1 buffer, the cyclic oxazolidone derivative is quantitated with a clenbuterol analogue as internal standard (NAB-760 CI). Confirmation is accomplished by comparison of ion ratios with those of a pure synthesized standard of clenbuterol oxazolidin-3-one obtained by selected ion monitoring, electron ionization gas chromatography/mass spectrometry on a benchtop instrument. Statistical information based on a series of standard curves for fortified tissues is included to describe method performance. Ion ratio variations were under 15%, and coefficients of variation for spiked tissue standard curves were above 0.997. Recoveries averaged 87.1 ± 6.6% for liver tissues across all 3 species and 67.1 ± 3.8% for muscle tissue across all 3 species.

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