Mutual separation of hinge-glycopeptide isomers bearing five N-acetylgalactosamine residues from normal human serum immunoglobulin A1 by capillary electrophoresis

Author(s):  
Hitoo Iwase ◽  
Atsushi Tanaka ◽  
Yoshiyuki Hiki ◽  
Tohru Kokubo ◽  
Takashi Sano ◽  
...  
1992 ◽  
Vol 22 (7) ◽  
pp. 1701-1706 ◽  
Author(s):  
Gilles Dietrich ◽  
Srinivas-Venkatesh Kaveri ◽  
Michel D. Kazatchkine

1982 ◽  
Vol 28 (1) ◽  
pp. 119-121 ◽  
Author(s):  
E Piall ◽  
G W Aherne ◽  
V Marks

Abstract We evaluated a commercially available (Diagnostic Biochemistry Inc.) doxorubicin 125I radioimmunoassay kit. This kit gave a high apparent doxorubicin concentration (greater than 12 micrograms/L), which was not linearly related to dilution, for two pools of normal human serum and plasma and also for samples collected from patients before they received the drug. In contrast, a doxorubicin 3H radioimmunoassay developed by us gave a low blank (2 micrograms/L), which was linearly related to dilution, for the same pools and patients' samples. Doxorubicin concentrations in the plasma of patients receiving the drug were compared by the two methods; the kit gave results five- to 10-fold those obtained with our assay. High nonspecific interference by serum and plasma as measured by the 125I radioimmunoassay must therefore be borne in mind by users of the kit, and we suggest that results should be corrected for these nonspecific effects.


Biochemistry ◽  
1963 ◽  
Vol 2 (2) ◽  
pp. 286-289 ◽  
Author(s):  
Walter N. Shaw ◽  
Eldon W. Shuey

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