Development and validation of an automated method for the liquid chromatographic determination of sotalol in plasma using dialysis and trace enrichment on a cation-exchange pre-column as on-line sample preparation

2001 ◽  
Vol 24 (5-6) ◽  
pp. 801-814 ◽  
Author(s):  
P Chiap ◽  
A Ceccato ◽  
B Miralles Buraglia ◽  
B Boulanger ◽  
P Hubert ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Cation Exchange ◽  
Chromatographic Determination ◽  
Automated Method ◽  
On Line ◽  
Liquid Chromatographic Determination ◽  
Development And Validation ◽  
Liquid Chromatographic ◽  
Line Sample

A Simple Automated Injection Technique for the High-Pressure Liquid Chromatographic Determination of Plasma Retinol

1983 ◽  
Vol 20 (5) ◽  
pp. 308-311 ◽  
Author(s):  
D W Howells ◽  
I R F Brown ◽  
O G Brooke ◽  
V Newey
Keyword(s):  
High Pressure ◽  
Chromatographic Determination ◽  
Injection Technique ◽  
High Pressure Liquid Chromatographic ◽  
Automated Method ◽  
Time Standards ◽  
Liquid Chromatographic Determination ◽  
Plasma Retinol ◽  
Liquid Chromatographic

We describe a rapid automated method for determination of retinol in 100 μl plasma. A lipid extract of plasma is analysed by reverse phase high pressure liquid chromatography using methanol-water as eluent. Automation of injection is achieved by adaptation of a Technicon AutoAnalyzer Sampler 2 coupled with a Micromedic dilution system and a pneumatically actuated loop injector. Twenty patients' specimens can be analysed in 4 hours, including extraction time, standards, and quality controls, with one extract injected every 4 minutes.

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