Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens

Noroviruses (NoVs) and rotaviruses (RVs), the two most important causes of viral acute gastroenteritis, are found to recognise histo-blood group antigens (HBGAs) as receptors or ligands for attachment. Human HBGAs are highly polymorphic containing ABO, secretor and Lewis antigens. In addition, both NoVs and RVs are highly diverse in how they recognise these HBGAs. Structural analysis of the HBGA-binding interfaces of NoVs revealed a conserved central binding pocket (CBP) interacting with a common major binding saccharide (MaBS) of HBGAs and a variable surrounding region interacting with additional minor binding saccharides. The conserved CBP indicates a strong selection of NoVs by the host HBGAs, whereas the variable surrounding region explains the diverse recognition patterns of different HBGAs by NoVs and RVs as functional adaptations of the viruses to human HBGAs. Diverse recognition of HBGAs has also been found in bacterial pathogenHelicobacter pylori. Thus, exploratory research into whether such diverse recognitions also occur for other viral and bacterial pathogens that recognise HBGAs is warranted.

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Characterization of murine monoclonal antibodies againstHelicobacter pylorilipopolysaccharide specific for Lexand Leyblood group determinants

Biochemistry and Cell Biology ◽

10.1139/o05-052 ◽

2005 ◽

Vol 83 (5) ◽

pp. 589-596 ◽

Cited By ~ 11

Author(s):

Eleonora Altman ◽

Blair A Harrison ◽

Tomoko Hirama ◽

Vandana Chandan ◽

Rebecca To ◽

...

Keyword(s):

Helicobacter Pylori ◽

Monoclonal Antibodies ◽

Blood Group ◽

Cell Envelope ◽

Mucosal Cell ◽

Blood Group Antigens ◽

Binding Characteristics ◽

Igg3 Subclass ◽

H Pylori

The cell envelope of Helicobacter pylori contains lipopolysaccharide (LPS), the O-chain of which expresses type 2 Lexand Leyblood group antigens, which mimic human gastric mucosal cell-surface glycoconjugates and may contribute to the survival of H. pylori in gastric mucosa. Here we describe the generation of monoclonal antibodies specific for Lexand Leyblood group determinants and the characterization of their binding properties using purified, structurally defined H. pylori LPS, synthetic glycoconjugates, and H. pylori cells. Analysis of oligosaccharide binding by SPR provided a rapid and reliable means for characterization of antibody affinities. One of the antibodies, anti-Lex, was of IgG3 subclass and had superior binding characteristics as compared with the commercially available anti-LexIgM. These antibodies could have potential in the immunodiagnosis of certain types of cancer, in serotyping of H. pylori isolates, and in structure–function studies.Key words: Helicobacter pylori, lipopolysaccharide, monoclonal antibodies, Lewis determinants, immunodiagnosis.

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Helicobacter pylori Adhesin Binding Fucosylated Histo-Blood Group Antigens Revealed by Retagging

Science ◽

10.1126/science.279.5349.373 ◽

1998 ◽

Vol 279 (5349) ◽

pp. 373-377 ◽

Cited By ~ 769

Author(s):

D. Ilver

Keyword(s):

Helicobacter Pylori ◽

Blood Group ◽

Blood Group Antigens

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Expression of histo-blood group antigens by lipopolysaccharides of Helicobacter pylori strains from Asian hosts: the propensity to express type 1 blood-group antigens

Glycobiology ◽

10.1093/glycob/10.7.701 ◽

2000 ◽

Vol 10 (7) ◽

pp. 701-713 ◽

Cited By ~ 39

Author(s):

M. A. Monteiro ◽

P.-y. Zheng ◽

B. Ho ◽

S.-i. Yokota ◽

K.-i. Amano ◽

...

Keyword(s):

Helicobacter Pylori ◽

Blood Group ◽

Blood Group Antigens

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Relationship of Blood Group Determinants on Helicobacter pylori Lipopolysaccharide with Host Lewis Phenotype and Inflammatory Response

Infection and Immunity ◽

10.1128/iai.68.2.937-941.2000 ◽

2000 ◽

Vol 68 (2) ◽

pp. 937-941 ◽

Cited By ~ 70

Author(s):

Michael A. Heneghan ◽

Ciaran F. McCarthy ◽

Anthony P. Moran

Keyword(s):

Helicobacter Pylori ◽

Inflammatory Response ◽

Blood Group ◽

Chronic Gastritis ◽

Bacterial Colonization ◽

Neutrophil Infiltration ◽

Blood Group Antigens ◽

Significant Difference ◽

H Pylori ◽

Relationship Of

ABSTRACT As Lewis a (Lea) and Lewis b (Leb) blood group antigens are isoforms of Lewis x (Lex) and Lewis y (Ley) and are expressed in the gastric mucosa, we evaluated whether the patterns of expression of Lex and Ley on Helicobacter pylorilipopolysaccharides reflected those of host expression of Lea and Leb. When 79 patients (secretors and nonsecretors) were examined for concordance between bacterial and host Le expression, no association was found (χ2 = 5.734, 3 df, P = 0.125), nor was there a significant difference between the amount of Lex or Ley expressed on isolates from ulcer and chronic gastritis patients (P > 0.05). Also, the effect of host and bacterial expression of Le antigens on bacterial colonization and the observed inflammatory response was assessed. In ulcer patients, Lex expression was significantly related to neutrophil infiltration (r s = 0.481,P = 0.024), whereas in chronic gastritis patients significant relationships were found between Lexexpression and H. pylori colonization density (r s = 0.296, P = 0.03), neutrophil infiltrate (r s = 0.409,P = 0.001), and lymphocyte infiltrate (r s = 0.389, P = 0.002). Furthermore, bacterial Ley expression was related to neutrophil (r s = 0.271, P= 0.033) and lymphocyte (r s = 0.277,P = 0.029) infiltrates. Thus, although no evidence of concordance was found between bacterial and host expression of Le determinants, these antigens may be crucial for bacterial colonization, and the ensuing inflammatory response appears, at least in part, to be influenced by Le antigens.

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Use of Real-Time, Label-Free Analysis in Revealing Low-Affinity Binding to Blood Group Antigens by Helicobacter pylori

Analytical Chemistry ◽

10.1021/ac201260c ◽

2011 ◽

Vol 83 (16) ◽

pp. 6336-6341 ◽

Cited By ~ 31

Author(s):

Y. Y. Fei ◽

A. Schmidt ◽

G. Bylund ◽

D. X. Johansson ◽

S. Henriksson ◽

...

Keyword(s):

Helicobacter Pylori ◽

Real Time ◽

Blood Group ◽

Affinity Binding ◽

Blood Group Antigens ◽

Label Free

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Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran

Journal of International Medical Research ◽

10.1177/03000605211058870 ◽

2021 ◽

Vol 49 (12) ◽

pp. 030006052110588

Author(s):

Azar Dokht Khosravi ◽

Mehrandokht Sirous ◽

Morteza Saki ◽

Sakineh Seyed-Mohammadi ◽

Seyed Reza Modares Mousavi ◽

...

Keyword(s):

Helicobacter Pylori ◽

Blood Group ◽

Blood Donors ◽

Blood Groups ◽

Blood Type ◽

Enzyme Linked Immunosorbent Assay ◽

Blood Group Antigens ◽

Chi Square ◽

Cytotoxin Associated Gene A ◽

H Pylori

Objective To investigate correlations between ABO/rhesus (Rh) blood group antigens and anti- Helicobacter pylori and anti-cytotoxin-associated gene A (CagA) seropositivity in blood donors. Methods A total of 311 blood donors were enrolled. ABO and Rh blood groups were determined using hemagglutination tests. Specific anti- H. pylori IgG and anti-CagA IgG antibodies in sera were quantitated by enzyme-linked immunosorbent assay. Correlations between blood groups and anti- H. pylori and anti-CagA seropositivity were evaluated using the Chi-square test. Results O+ was the most frequent blood type (38%, n = 118). Anti- H. pylori IgG seropositivity was observed in 240 (77.2%) blood donors, while anti-CagA IgG seropositivity was observed in 132 (42.5%) blood donors. Although seropositivity rates for both anti- H. pylori and anti-CagA IgG were higher in individuals with blood type O, no statistically significant associations were observed between seropositivity and any ABO/Rh blood groups. Conclusion Individuals with blood type O may have higher rates of H. pylori seropositivity.

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