PCR Targeting of Antibiotic Resistant Bacteria in Public Drinking Water of Lahore Metropolitan, Pakistan

2009 ◽  
Vol 22 (6) ◽  
pp. 458-463 ◽  
Author(s):  
Zahoor Qadir SAMRA ◽  
Mariam NASEEM ◽  
Sumaria Javed KHAN ◽  
Nadia DAR ◽  
Muhammad Amin ATHAR
1981 ◽  
Vol 42 (2) ◽  
pp. 277-283 ◽  
Author(s):  
J L Armstrong ◽  
D S Shigeno ◽  
J J Calomiris ◽  
R J Seidler

1987 ◽  
Vol 32 (1-2) ◽  
Author(s):  
HelmyTawfik El-Zanfaly ◽  
El-SaiedAbd-Aziz Kassim ◽  
SamirM. Badr-Eldin

Author(s):  
Lina Wallmann ◽  
Jörg Krampe ◽  
Josef Lahnsteiner ◽  
Elena Radu ◽  
Pierre van Rensburg ◽  
...  

Abstract Given the availability of technological solutions and guidelines for safe drinking water, direct potable reuse of reclaimed water has become a promising option to overcome severe lack of potable water in arid regions. However, the growing awareness of the presence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG) in corresponding raw wastes has led to new safety concerns. This study investigated the fate of ARB and intracellular and extracellular ARG after each treatment step of an advanced water treatment facility in Windhoek, Namibia. The New Goreangab Water Reclamation Plant (NGWRP) produces drinking water from domestic secondary wastewater treatment plant effluent and directly provides for roughly a quarter of Windhoek's potable water demand. Procedures to study resistance determinants were based on both, molecular biology and culture-based microbiological methods. TaqMan real-time PCR was employed to detect and quantify intracellular resistance genes sul1, ermB, vanA, nptII and nptIII as well as extracellular resistance gene sul1. The NGWRP reduced the amount of both culturable bacterial indicators as well as the resistance genes to levels below the limit of detection in the final product. The main ozonation and the ultrafiltration had the highest removal efficiencies on both resistance determinants.


2016 ◽  
Vol 3 (3) ◽  
pp. 541-552 ◽  
Author(s):  
Sadia Khan ◽  
Charles W. Knapp ◽  
Tara K. Beattie

1991 ◽  
Vol 24 (2) ◽  
pp. 43-48 ◽  
Author(s):  
Helmy T. El-Zanfaly

The microbiological criteria for water quality have been directed towards protection of consumers from possible microbial pollution which may cause public health hazards. Therefore, the bacteriological standards for drinking water are based mainly on bacterial indicators. Another problem of prime concern to public health is the presence of antibiotic-resistant bacteria in drinking water Antibiotic–resistant bacteria were found among standard plate count populations of Chlorinated drinking water from two districts in Cairo. Most strains appeared to be ampicillin resistant (89%). Those were followed by sulfaguanidine (78%) and streptomycin (57%). The majority of the tested strains (62.4 to 98%) were multiple antibiotic resistant (MAR). Identification of 363 MAR strains revealed that Gram-positive rods were dominant, while Gram-negative fermentative rods, Gram-positive cocci and Gram-negative nonfermentative rods represent the second, third and fourth group. A total of 101 strains isolated from underground water pumped from three water works in Cairo were classified and tested for their resistance towards four commonly used antibiotics; chloramphenicol, tetracycline, neomycin, penicillin and one chemotherapeutic agent namely sulfanilamide pyrimidine. Results showed that 77, 64 and 32 isolates have resistance towards penicillin, sulfanilamide pyrimidine and tetracycline, respectively. Only 18 and 8 isolates were resistant to chloramphenicol and neomycin, respectively. It was also found that 19 isolates belonging to 6 genera or groups were sensitive towards all of the tested compounds. Therefore, any source of antibiotic~resistant bacteria must be viewed with concern and use of data on MAR bacteria should be made in future water quality deliberations and in regulating effluent quality discharges.


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