Osteoinductive potential of freeze-dried, biodegradable, poly(glycolic acid-co-lactic acid) disks incorporated with bone morphogenetic protein in skull defects of rats

1996 ◽  
Vol 25 (5) ◽  
pp. 402-406 ◽  
Author(s):  
Takashi Miki ◽  
Yohji Imai
Keyword(s):  
Lactic Acid ◽  
Bone Morphogenetic Protein ◽  
Glycolic Acid ◽  
Osteoinductive Potential ◽  
Skull Defects ◽  
Morphogenetic Protein ◽  
Freeze Dried ◽  
Biodegradable Poly

scholarly journals Transplanted Bone Morphogenetic Protein/Poly(lactic-co-glycolic Acid) Delayed-release Microcysts Combined with Rat Micromorselized Bone and Collagen for Bone Tissue Engineering

2009 ◽  
Vol 37 (4) ◽  
pp. 1075-1087 ◽  
Author(s):  
Y Ji ◽  
GP Xu ◽  
JL Yan ◽  
SH Pan
Keyword(s):  
Bone Morphogenetic Protein ◽  
Glycolic Acid ◽  
Gluteus Maximus ◽  
The Other ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Collagen ◽  
Delayed Release ◽  
Morphogenetic Protein ◽  
Gluteus Maximus Muscle ◽  
Autologous Bone

This study was designed to optimize the preparation of delayed-release microcysts containing bone morphogenetic protein 2 (BMP-2) combined with poly(lactic- co-glycolic acid) (PLGA) and to investigate their osteogenic properties when combined with rat autologous micromorselized bone and collagen. Rat autologous micromorselized bone, collagen and BMP-2/PLGA delayed-release microcysts were implanted in various combinations into the rat gluteus maximus muscle sack model. The following post-operative measurements were made: general observations of the implant site, histological observations, osteogenesis measurements and alkaline phosphatase activity. Autologous micromorselized bone combined with collagen and BMP-2/PLGA delayed-release microcysts demonstrated significantly superior osteogenic properties than any of the other combinations of these three components. These findings suggest that micromorselized bone combined with collagen and BMP-2/PLGA delayed-release microcysts could reduce the quantity of BMP-2 and autologous bone required for these procedures, making their use feasible in human bone restoration.

Experimental Myringoplasty

1980 ◽  
Vol 3 (6) ◽  
pp. 354-357 ◽  
Author(s):  
L. Feenstra ◽  
B.W.C. Van Der Ven ◽  
F.E. Kohn ◽  
J. Feijen
Keyword(s):  
Lactic Acid ◽  
Bisphenol A ◽  
Tympanic Membrane ◽  
Biodegradable Polymers ◽  
Glycolic Acid ◽  
Tissue Reaction ◽  
Biodegradable Poly ◽  
One Year ◽  
Poly Carbonate ◽  
Tympanic Membranes

Artificial eardrums made from biodegradable poly(D, L-lactic acid), poly(glycolic acid) and poly(ß-benzyl-L-aspartate-co-L-leucine) 50/50, and made from the microporous poly(tetrafluoroethylene) and bisphenol-A poly(carbonate) membranes were implanted into the ear and as a reference subcutaneously in rats. The implants were histologically examined for periods up to one year. From the biodegradable polymers studied the poly(ß-benzyl-L-aspartate-co-L-leucine) 50/50 evoked the least tissue reaction and the newly formed tympanic membranes are the best in terms of thickness and overall integrity. The microporous poly(tetrafluoroethylene) membrane can be considered as a valuable support for the formation of a reinforced tympanic membrane.

scholarly journals Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 inBacillus subtilis

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Muhammad Umair Hanif ◽  
Roquyya Gul ◽  
Muhammad Imran Hanif ◽  
Abid Ali Hashmi
Keyword(s):  
Bacillus Subtilis ◽  
Bone Morphogenetic Protein ◽  
C2c12 Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Dependent Manner ◽  
Secretory Expression ◽  
Native Page ◽  
Alkaline Phosphatase Assay ◽  
Morphogenetic Protein ◽  
Freeze Dried

Recombinant human Bone Morphogenetic Protein 2 (rhBMP2) has important applications in the spine fusion and ortho/maxillofacial surgeries. Here we first report the secretory expression of biological active dimerized rhBMP2 fromBacillus subtilissystem. The mature domain of BMP2 gene was amplified from pTz57R/BMP2 plasmid. By using pHT43 expression vector two constructs, pHT43-BMP2-M (single BMP2 gene) and pHT43-BMP2-D (two BMP2 genes coupled with a linker to produce a dimer), were designed. After primary cloning (DH5αstrain) and sequence analysis, constructs were transformed intoBacillus subtilisfor secretory expression. Expression conditions like media (2xYT) and temperature (30°C) were optimized. Maximum 35% and 25% secretory expression of monomer (~13 kDa) and dimer (~25 kDa), respectively, were observed on SDS-PAGE in SCK6 strain. The expression and dimeric nature of rhBMP2 were confirmed by western blot and native PAGE analysis. For rhBMP2 purification, 200 ml culture supernatant was freeze dried to 10 ml and dialyzed (Tris-Cl, pH 8.5) and Fast Protein Liquid Chromatography (6 ml, Resource Q column) was performed. The rhBMP2 monomer and dimer were eluted at 0.9 M and 0.6 M NaCl, respectively. The alkaline phosphatase assay of rhBMP2 (0, 50, 100, 200, and 400 ng/ml) was analyzed on C2C12 cells and maximum 200 ng/ml activity was observed in dose dependent manner.

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