P1858 Molecular typing and pathogenic potential of Listeria monocytogenes isolates from food and clinical origin

2007 ◽  
Vol 29 ◽  
pp. S532
Author(s):  
N. Papageorgiou ◽  
E. Scoulica ◽  
C. Panoulis ◽  
S. Maraki ◽  
A. Christidou ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Pathogenic Potential

Analysis of virulence genes and molecular typing of Listeria monocytogenes isolates from human, food, and livestock from 2008 to 2016 in Iran

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Masoud Naghizadeh Heidarlo ◽  
Lida Lotfollahi ◽  
Saber Yousefi ◽  
Vahid Lohrasbi ◽  
Gholamreza Irajian ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Virulence Genes ◽  
Human Food

scholarly journals Listeria monocytogenes Subgroups IIIA, IIIB, and IIIC Delineate Genetically Distinct Populations with Varied Pathogenic Potential

2006 ◽  
Vol 44 (11) ◽  
pp. 4229-4233 ◽  
Author(s):  
D. Liu ◽  
M. L. Lawrence ◽  
M. Wiedmann ◽  
L. Gorski ◽  
R. E. Mandrell ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Pathogenic Potential

Low Potential Virulence Associated with Mutations in the inlA and prfA Genes in Listeria monocytogenes Isolated from Raw Retail Poultry Meat

2013 ◽  
Vol 76 (1) ◽  
pp. 129-132 ◽  
Author(s):  
VICTORIA LÓPEZ ◽  
JAIME NAVAS ◽  
JOAQUÍN V. MARTÍNEZ-SUÁREZ
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Virulence Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Poultry Meat ◽  
Pathogenic Potential ◽  
Molecular Serotyping ◽  
Potential Source ◽  
Raw Foods ◽  
Over Time

Packaged raw foods can represent a potential source of Listeria monocytogenes contamination when opened at home, and listeriosis is associated with the consumption of undercooked raw foods. The aim of this study was to characterize a group of L. monocytogenes strains isolated from 56 packages of raw chicken meat from a single brand in order to determine the diversity of the strains that dominate in a particular food over time, as well as their pathogenic potential. Forty (71%) samples were found to be positive for L. monocytogenes, and three isolates per sample were subjected to PCR molecular serotyping. Subtyping of 45 isolates from different manufacturing dates (n = 40) or different molecular serotype within the same sample (n = 5) identified 11 different L. monocytogenes subtypes as defined by pulsed-field gel electrophoresis and sequencing of virulence genes actA and inlA. Two of the subtypes accounted for 51% of the isolates. About 40% of isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes.

scholarly journals Assessment of the pathogenic potential of two Listeria monocytogenes human faecal carriage isolates The GenBank accession number for the sequence reported in this paper is AF468816.

Microbiology ◽  
2002 ◽  
Vol 148 (6) ◽  
pp. 1855-1862 ◽  
Author(s):  
Maı̈wenn Olier ◽  
Fabrice Pierre ◽  
Jean-Paul Lemaı̂tre ◽  
Charles Divies ◽  
André Rousset ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Accession Number ◽  
Pathogenic Potential ◽  
Faecal Carriage

scholarly journals Recurrent and Sporadic Listeria monocytogenes Contamination in Alheiras Represents Considerable Diversity, Including Virulence-Attenuated Isolates

2007 ◽  
Vol 73 (12) ◽  
pp. 3887-3895 ◽  
Author(s):  
M. T. S. Fel�cio ◽  
T. Hogg ◽  
P. Gibbs ◽  
P. Teixeira ◽  
M. Wiedmann
Keyword(s):  
Listeria Monocytogenes ◽  
Control Strategies ◽  
Tetracycline Resistance ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Pathogenic Potential ◽  
Molecular Serotyping ◽  
Production And Distribution ◽  
Invasion Assays

ABSTRACT Microbiological characterization of alheiras, traditional smoked meat sausages produced in northern Portugal, had previously shown that more than 60% of the lots analyzed were contaminated with Listeria monocytogenes at levels higher than 100 CFU/g. In order to better understand L. monocytogenes contamination patterns in alheiras, we characterized 128 L. monocytogenes isolates from alheiras using a variety of subtyping techniques (i.e., molecular serotyping; arsenic, cadmium, and tetracycline resistance typing; and pulsed-field gel electrophoresis [PFGE]). Subtyping of isolates from products collected on two separate dates provided evidence for the persistence of specific L. monocytogenes PFGE types in the production and distribution chains of alheiras from four different processors. A subset of 21 isolates was further characterized using ribotyping and Caco-2 cell invasion assays to evaluate the pathogenic potential of L. monocytogenes present in alheiras. Caco-2 invasion assays revealed seven isolates with invasion efficiencies that were less than 20% of that of the control strain 10403S. All seven isolates had premature stop codons in inlA that represented three distinct mutations, which had previously been observed in isolates from the United States or France. Our findings indicate the need for a comprehensive approach to control L. monocytogenes in alheiras, including strategies to reduce persistence. The presence of considerable diversity in invasion phenotypes among L. monocytogenes strains present in alheiras, including the presence of subtypes likely to be virulence attenuated, may provide an opportunity to initially focus control strategies on the subtypes most likely to cause human disease.

scholarly journals A Novel Methicillin-ResistantStaphylococcus aureust11469 and a Poultry Endemic Strain t002 (ST5) Are Present in Chicken in Ebonyi State, Nigeria

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Amos Nworie ◽  
Azi S. Onyema ◽  
Simon I. Okekpa ◽  
Michael O. Elom ◽  
Nse O. Umoh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Virulence Factor ◽  
Molecular Typing ◽  
Staphylococcal Infection ◽  
Sequence Type ◽  
Antimicrobial Use ◽  
Putative Virulence Factor ◽  
The Novel ◽  
Pathogenic Potential ◽  
Endemic Strain

Background. The changing epidemiology of methicillin-resistantStaphylococcus aureus(MRSA) from a hospital-associated pathogen to an organism commonly found in the community and in livestock reflects an organism well-equipped to survive in diverse environments and adjust to different environmental conditions including antimicrobial use.Methods. We investigated the molecular epidemiology ofS. aureusand MRSA in poultry in Ebonyi State, Nigeria. Samples were collected from 1800 birds on 9 different farms within the state. Positive isolates were tested for antibiotic susceptibility and molecular typing.Results. Prevalence in birds was 13.7% (247/1800). MRSA prevalence in poultry was 0.8%. The prevalence of MRSA in broilers and layers was 1.2% and 0.4%, respectively. All tested isolates were susceptible to vancomycin. Molecular analysis of the isolates revealed 3spatypes: t002, t084, and a novelspatype, t11469. The novelspatype t11469 belonged to sequence type ST5.Conclusion. The detection of t002 in chicken suggests the presence of livestock-associated MRSA in poultry in Ebonyi State. The detection of the newspatype t11469 in poultry that has not been characterised to ascertain its pathogenic potential remains a cause for concern, especially as some were found to carry PVL genes, a putative virulence factor in staphylococcal infection.

scholarly journals More than one variant ofListeria monocytogenesisolated from each of two human cases of invasive listeriosis

2006 ◽  
Vol 135 (5) ◽  
pp. 854-856 ◽  
Author(s):  
W. THAM ◽  
G. LOPEZ VALLADARES ◽  
S. HELMERSSON ◽  
A. ÖSTERLUND ◽  
M.-L. DANIELSSON-THAM
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Blood Cultures ◽  
Pulsed Field Gel Electrophoresis ◽  
The Other ◽  
Dna Profile ◽  
Pulsed Field ◽  
Different Strains

SUMMARYTwo variants ofListeria monocytogeneswere isolated from blood cultures from each of two patients with listeriosis. Each variant displayed a two-band difference in DNA profile from the other by pulsed-field gel electrophoresis. Although this difference in profile is insufficient to distinguish clearly between the variants, the possibility of co-infection with different strains ofL. monocytogenesneeds to be considered. We suggest that more than one colony should be selected for molecular typing to aid interpretation during investigation of the sources and routes ofListeriainfection.

scholarly journals Comparación teórica entre técnicas fenotípicas y genotípicas utilizadas en la identificación de Listeria monocytogenes

2021 ◽  
Vol 16 (2) ◽  
pp. 7-19
Author(s):  
Adriana Giraldo Aristizábal ◽  
Astrid Maribel Aguilera Becerra ◽  
Eliana Ximena Urbano Cáceres ◽  
Adriana María Pedraza Bernal ◽  
Claudia Patricia Jaimes Bernal
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Bacterial Typing

Listeria monocytogenes es un patógeno ubicuo intracelular, causante de la Listeriosis, la cual se considera una enfermedad transmitida por alimentos (ETA). En la actualidad existe una creciente demanda de consumidores de productos alimenticios tratados mínimamente que pueden favorecer la proliferación de este microorganismo. Es necesario contar con programas de vigilancia que incluyan métodos fiables para la detección de este patógeno en casos de brotes epidémicos. Esta revisión bibliográfica compara las ventajas y desventajas de las técnicas fenotípicas y genotípicas utilizadas en la determinación de L. monocytogenes con el fin de definir la más adecuada que permita obtener resultados confiables y en el menor tiempo posible. Se realizó una búsqueda bibliográfica en bases de datos como Pubmed, Science Direct, Proquest y Ovid, en inglés y español, utilizando los siguientes descriptores: L. monocytogenes, molecular typing, diagnosis, PCR y bacterial typing techniques. Estos se combinaron de diferentes maneras para, finalmente, recopilar setenta artículos que cumplieron con los criterios de selección propuestos. Como resultado se presentan las técnicas de diagnóstico fenotípico y genotípico que representan una opción útil para el aislamiento e identificación de este patógeno a partir de diferentes orígenes. Las técnicas revisadas permiten la diferenciación entre especies patógenas y no patógenas, así como de serotipos y genotipos con base en la implementación de procedimientos cuya fundamentación puede diferir, pero que igualmente pueden ser complementarias.

Distribution of Epidemic Clonal Genetic Markers among Listeria monocytogenes 4b Isolates

2007 ◽  
Vol 70 (3) ◽  
pp. 574-581 ◽  
Author(s):  
GIOVANNA FRANCIOSA ◽  
CONCETTA SCALFARO ◽  
ANTONELLA MAUGLIANI ◽  
FRANCESCA FLORIDI ◽  
ANTONIETTA GATTUSO ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Markers ◽  
Pulsed Field Gel Electrophoresis ◽  
Genomic Diversity ◽  
Clinical Isolates ◽  
Specific Marker ◽  
Environmental Isolates ◽  
Environmental Persistence ◽  
Pathogenic Potential ◽  
Serotype 4B

Recent genome sequencing of isolates of Listeria monocytogenes serotype 4b implicated in some major outbreaks of foodborne listeriosis has revealed unique genetic markers in these isolates. The isolates were grouped into two distinct epidemic clones, ECI and ECII. In the present study, selected ECI- and ECII-specific genetic markers were detected in 16 and 15 of 89 L. monocytogenes 4b isolates, respectively. The ECI markers were found in 6 of 34 clinical isolates, 9 of 50 food isolates, and 1 of 5 environmental isolates, and the ECII markers were detected in 7 of 34 clinical isolates, 7 of 50 food isolates, and 1 of 5 environmental isolates. Hence, of the isolates with the epidemic clonal genetic markers, 38% (13 of 34) were of clinical origin, 32% (16 of 50) were of food origin, and 40% (2 of 5) were of environmental origin. The predominance of the epidemic clonal markers among the clinical and environmental isolates supports the hypothesis that these markers are correlated with the pathogenic potential of strains and with their environmental persistence. Several isolates had only one epidemic clonal marker, either the ECI-specific marker 133 or the ECII-specific marker 4bSF18. Pulsed-field gel electrophoresis analysis revealed higher genomic diversity among the strains with ECII-like characteristics than among those strains carrying the ECI-specific genetic markers.

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